魏玉婷,王小洁,麦康森,艾庆辉

• 1:中国海洋大学海水养殖教育部重点实验室

摘要(Abstract)：

采用6种维生素E(VE)含量为26、52、66、132、191、339 mg/kg饲料干质量的等氮等能实验饲料,研究饲料中维生素E对大菱鲆(Scophthalmus maximus)幼鱼生长,肝脏和肌肉中的硫代巴必妥酸反应底物(TBARS)含量、超氧化物歧化酶(SOD)活性及总抗氧化力(T-AOC)的影响。结果表明大菱鲆的生长和体成分受饲料中VE含量的影响产生的差异不显著,然而肝脏和血清中的VE含量以及肝脏和肌肉中的硫代巴必妥酸底物(TBARS)含量、超氧化物歧化酶(SOD)活性和肝脏总抗氧化力(T-AOC)则受其影响显著(P<0.05)。结果显示大菱鲆幼鱼摄取不适宜量的VE时在表现出各种缺乏症之前会先发生脂肪过氧化现象,同时饲料中的VE能够有效提高大菱鲆幼鱼的抗氧化能力。

关键词(KeyWords)： 大菱鲆幼鱼;维生素E;脂肪过氧化;抗氧化能力

Abstract:

Keywords:

基金项目(Foundation): 公益性行业(农业)科研专项经费(Nyhy.207-046)资助

作者(Author): 魏玉婷,王小洁,麦康森,艾庆辉

DOI: 10.16441/j.cnki.hdxb.2011.06.008

参考文献(References)：

• [1]雷霁霖.大菱鲆养殖技术[M].上海:上海技术出版社,2003.
• [2]陈慕雁,张秀梅,连建华.大菱鲆仔稚鱼期消化酶及碱性磷酸酶活性的变化[J].中国海洋大学学报:自然科学版,2005,35(3):483-486.
• [3]李筠,颜显辉,陈吉祥,等.养殖大菱鲆腹水病病原的研究[J].中国海洋大学学报:自然科学版,2006,36(4):649-654.
• [4]Lee J K,Cho S H,Park S U,et al.Dietary protein requirementfor young turbot(Scophthalmus maximusL.)[J].AquacultureNutrition,2003,9:283-286.
• [5]Cho S H,Lee S M,Lee S M,et al.Effect of dietary protein andlipid levels on growth and body composition of juvenile turbot(Scophthalmus maximusL)reared under optimum salinity andtemperature conditions[J].Aquaculture Nutrition,2005,11(4):235-240.
• [6]Peres H,Oliva-Teles A.Lysine requirement and efficiency of ly-sine utilization in turbot(Scophthalmus maximus)juveniles[J].Aquaculture,2008,275:283-290.
• [7]陈京华.米曲霉AS 3.951发酵豆粕对大菱鲆摄食生长的影响[J].中国农学通报,2009,25(02):277-282.
• [8]Peres H,Oliva-Teles A.The effect of dietary protein replacementby crystalline amino acid on growth and nitrogen utilization of tur-botScophthalmus maximusjuveniles[J].Aquaculture,2005,250:755-764
• [9]郝林华,孙丕喜,石红旗,等.牛蒡寡糖对大菱鲆生长和免疫机能的影响[J].海洋科学进展,2007,25(2):208-214.
• [10]Halver J E.The vitamins.Fish nutrition[M].San Diego:Aca-demic Press,2002:61-141.
• [11]Huang C H,Huang S L.Effect of dietary vitamin E on growth,tissue lipid peroxidation,and liver glutathion level of juvenile hy-brid tilapia,Oreochromis niloticus×O.aureus,fed oxidized oil[J].Aquaculture,2004,237:381-389.
• [12]Sau S K,Paul B N,Mohanta K N,et al.Dietary vitamin E re-quirement,fish performance and carcass composition of rohu(Labeo rohita)fry[J].Aquaculture,2004,240:359-368.
• [13]Cowey C B,Adron J W,Walton M J,et al.Tissue distribution,uptake,and requirement for-αtocopherol of rainbow trout(Sal-mo gairdneri)fed diets with a minimal content of unsaturated fat-ty acids[J].Journal of Nutrition,1981,111:1556-1567.
• [14]Huang C H,Chang R J,Sue-Lan,et al.Dietary vitamin E sup-plementation affects tissue lipid peroxidation of hybrid tilapia,Oreochromis niloticus×O.aureus[J].Comparative Biochemistryand Physiology,Part B,2003,134:265-270.
• [15]Huang C H,Higgs D A,Balfry S K,et al.Effect of dietary vita-min E level on growth,tissue lipid peroxidation,and erythrocytefragility of transgenic coho salmon,Oncorhynchus kisutch[J].Comparative Biochemistry and Physiology,Part A,2004,139:199-204.
• [16]Baker R T M,Davies S J.Oxidative nutritional stree associatedwith feeding rancid oils to African catfish,Clarias gariepinus(Burchell)and the protective role of-αtocopherol[J].Aquacul-ture Research,1996,27(10):795-803.
• [17]Tocher D R,Mourente G,Eecken A V D,et al.Effects of dieta-ry vitamin E on antioxidant defence mechanisms of juvenile turbot(Scophthalmus maximusL.),halibut(Hippoglossus hippoglos-susL.)and sea bream(Sparus aurataL.)[J].Aquaculture Nu-trition,2002,8:195-207.
• [18]Lee M H,Shiau S Y.Vitamin E requirements of juvenile grassshrimp,Penaeus monodon,and effects on non-specific immuneresponses[J].Fish&Shellfish Immunology,2004,16:475-485.
• [19]邵裕坤,徐军,王珏.反相高效液相色谱法同时测定血清中维生素A,D3,E[J].中国现代应用药学杂志,2005,22(6):503-505.
• [20]Salo-Vaananen P,Ollilainen V,Mattila P,et al.SimultaneousHPLC analysis of fat-soluble vitamins in selected animal productsafter small-scale extraction[J].Food Chemistry,2000,71:535-543.
• [21]Wilson R P,Bowser P R,Poe W E.Dietary vitamin E require-ment of fingerling Channel Catfish[J].Journal of Nutrition,1984,114:2053-2058.
• [22]Musalmah M,Fairuz A H,Gapor M T,et al.Effect of vitaminE on plasma malondialdehyde,antioxidant enzyme levels and therates of wound closures during wound healing in normal and dia-betic rats[J].Asia Pacific J Clin Nutr,2002,11:448-451
• [23]Lin Y H,Shiau S Y.Dietary vitamin E requirement of grouper,Epinephelus malabaricus,at two lipid levels,and their effects onimmune responses[J].Aquaculture,2005,248:235-244.
• [24]Kocabas,Gatlin III.Dietary vitamin E requirement of hybridstriped bass(Morone chrysopsfemale×M.saxatilismale)[J].Aquaculture Nutrtition,1999,5(1):3-7.
• [25]朱勇飞,吴天星,张晓东.饲料维生素E对黑鲷抗氧化能力和鱼肉货架期的影响[J].水生态学杂志,2009:2(3):131-135
• [26]万敏,麦康森,马洪明,等.硒和维生素E对皱纹盘鲍血清抗氧化酶活力的影响[J].水生生物学报,2004,28(5):496-503.

文章评论(Comment)：