基于线粒体12S rRNA序列探讨4种青蟹系统发育关系及中国沿海青蟹的分类地位Study on Phylogenetic Relationships of Four Scylla Species and Taxonomic Status of Mud Crab in China Based on Mitochondrial 12S rRNA Sequences
高天翔,王玉江,刘进贤,马凌波,今井秀行
摘要(Abstract):
研究青蟹属4种青蟹的系统发育关系,阐明分布于中国沿海青蟹的分类地位,本研究采集了青蟹属4种青蟹以及中国沿海的青蟹样品,测定分析了其线粒体12S rRNA部分片段序列。结果表明4种青蟹的12S rRNA基因片段长度存在差异,每一种的个体都单独聚为1支,中国沿海的青蟹样品全部与Scylla paramamosain聚为1支,结果提示分布于中国沿海的青蟹主要是S.paramamosain。S.serrata,S.paramamosain和S.tranquebarica3个种间的亲缘关系较近,这3个种形成1个单系群并且具有很高的自展置信值。S.olivacea与其他3个种互为单系,与以上3种青蟹的亲缘关系较远。
关键词(KeyWords): 青蟹;12SrRNA;系统发育;分类
基金项目(Foundation): 农业部海洋与河口渔业重点开放实验室开放课题(开-05-01-2)资助
作者(Author): 高天翔,王玉江,刘进贤,马凌波,今井秀行
DOI: 10.16441/j.cnki.hdxb.2007.01.010
参考文献(References):
- [1]戴爱云,杨思谅,宋玉枝,等.中国海洋蟹类[M].北京:海洋出版社,1986:190-191.
- [2]Li S,Zeng C,Tang H,et al.Investigations into the reproductive andlarval culture biology of the mud crab,Scylla paramamosain:a re-search overview[J].ACIAR Proceedings,1999,78:121-124.
- [3]吴琴瑟.锯缘青蟹人工养殖技术[J].海洋渔业,1991,5:209-211.
- [4]吴琴瑟.锯缘青蟹繁殖生物学的研究[J].湛江海洋大学学报,2002,1:13-17.
- [5]Estampador E P.Studies onScylla(Crustacea:Portunidae),I.Re-vision of the genus[J].Philippine Journal of Science,1949,78:95-108.
- [6]Keenan C P.The fourth species ofScylla[J].ACIAR Proceedings,1999,78:48-58.
- [7]Overton J L.Morphometrics and ecology of the mud crab(Scyllaspp.)from Southeast Asia[J].ACIAR Proceedings,1999,78:35-42.
- [8]Sugama K,Hutapea J H.Genetic characterization in the mud crabScylla(Brachyura:Portunidae)[J].ACIAR Proceedings,1999,78:43-47.
- [9]王玉江,高天翔,韩志强,等.中国和越南青蟹线粒体16S rRNA基因序列分析[J].中国海洋大学学报:自然科学版,2005,35(4):554-558.
- [10]Fuseya R,Watanabe S.Genetic variability in the mud crab genusScylla(Decapoda:Portunidae)[J].Fisheries Science,1996,1:95-109.
- [11]Brown W M.Evolution of animal mitochondrial DNAs[M].//NeiM,Koehned R K eds.Evolution of Genes and Proteins.Sinauer:Sunderland MA,1983.
- [12]Lu G,Li S.Advances in the study and application of fish mitochon-drial DNA polymorphism[J].Journal of Fisheries of China,1998,3:94-103.
- [13]Keenan C P,Davies P J F,Mann D L.A revision of the genusScyllade Haan,1833(Crustacea:Decapoda:Brachyura:Portu-nidae)[J].Raffles Bulletin of Zoology,1998,46:217-245.
- [14]Imai H,Numachi K.Intra-and interspecific genetic variability andrelationships among mud crabs,Scyllaspp.(Decapoda:Portu-nidae),demonstrated by RFLP analysis of mitochondrial DNA[J].Journal of Animal Genetics,2002,29(2):3-11.
- [15]Imai H,Cheng J,Hamasaki K,et al.Identification of four mudcrab species(genusScylla)using ITS-1 and 16S rDNA markers[J].Aquatic Living Resources,2004,1:31-34.
- [16]高天翔,王玉江,刘进贤,等.三种青蟹线粒体12S rRNA基因序列分析[J].水产学报,2005,29(3):313-317.
- [17]黎中宝,李少菁,王桂忠.锯缘青蟹(Scylla serrata)不同种群的杂合性研究[J].海洋与湖沼,2004,35(4):358-363.
- [18]Li Z,Li S,Wang G.Genetic diversity and differentiation of mudcrabScylla serratapopulations from southeastern China[J].ActaOceanologica Sinica,2004,23(2):309-316.
- [19]Kumar S,Tamura K,Jakobsen I B,et al.MEGA2:MolecularEvolutionary Genetics Analysis software[J].Bioinformatics,2001,17:1244-1245.
- [20]施心展.4种青蟹(Scyllaspp.)在外部形态上及核糖体DNA上之差异[D].台湾:国立中山大学海洋生物研究所,2002.
- [21]左荣升.4种青蟹大眼幼体在外部形态及族群动态上之差异[D].台湾:国立中山大学海洋生物研究所硕士学位论文.2002.
- [22]杉本昌彦.アイソザイムを指标としたノコギリガザミ属の群解析[R].高知县日本:高知县水产试验场报告书,1990:18-22.
文章评论(Comment):
|
||||||||||||||||||
|
||||||||||||||||||