杨萌,陈琳,赵阳国,白洁,张彦超

• 1:中国海洋大学环境科学与工程学院

摘要(Abstract)：

以沸石为载体,吸附固定化耐盐氨氧化-反硝化菌株,探讨固定化菌株对氨氮的去除能力;创建海水养殖系统,通过投加固定化脱氮菌株,分析其对养殖水体中的氨氮控制效果及其影响因素。结果表明,沸石固定化菌剂的含菌量为1.55×1010 CFU/g,对氨氮的控制效果受溶解氧、环境温度和菌剂投加量的影响较大,用沸石固定后菌剂对温度变化有更好的耐受性。当溶解氧含量大于6.5mg/L,温度为30℃,在50mL体系中加2g沸石固定化的微生物菌剂,24h时,最高可去除90mg/L的氨氮。在海水养殖环境中,将沸石固定化菌剂平铺于土工布袋内,将布袋覆盖于沉积物-水界面,覆盖率2.5%,发现固定化菌剂在9d内能够显著抑制水中氨氮的产生,对氨氮的控制率达到(73±17)%。本研究表明该固定化脱氮菌株能够很好的降低养殖水体中氨氮浓度,为其在海水养殖系统中的应用提供了数据支撑。

关键词(KeyWords)： 脱氮菌;固定化;海水养殖;生态修复

Abstract:

Keywords:

基金项目(Foundation): 山东省科技攻关项目(2016GSF115004)资助~~

作者(Author): 杨萌,陈琳,赵阳国,白洁,张彦超

DOI: 10.16441/j.cnki.hdxb.20180075

参考文献(References)：

• [1]张卫强,朱英.养殖水体中氨氮的危害及其检测方法研究进展[J].环境卫生学杂志,2012,2(6):324-327.Zhang W Q,Zhu Y.Advances on the research of the hazard of ammonia nitrogen in aquaculture water and its determination method[J].Journal of Environmental Hygiene Dec,2012,2(6):324-327.
• [2]董乔仕.养殖水体氨氮的危害及改良[J].齐鲁渔业,2002,19(9):9-10.Dong Q S.Harm and improvement of ammonia nitrogen in breeding water[J].Shandong Fisheries,2002,19(9):9-10.
• [3]周平.氨氮对水产养殖的危害及防治措施[J].中国水产,2013(8):63-64.Zhou P.Harmfulness of ammonia nitrogen to aquaculture and its control measures[J].Chinese Journal of Fisheries,2013(8):63-64.
• [4]王彦波,许梓荣,邓岳松.水产养殖中氨氮和亚硝酸盐氮的危害及治理[J].饲料工业,2002,23(12):46-48.Wang Y B,Xu Z R,Deng Y S.Harm and control of ammonia nitrogen and nitrite nitrogen in aquaculture[J].Feed Industry,2002,23(12):46-48.
• [5]冯敏毅,马甡,文国樑,等.水产养殖环境生物修复技术的研究进展[J].海洋科学,2006,30(9):84-87.Feng Y M,Ma S,Wen G D,et al.Advances in bioremediation researches on aquacultural environment[J].Marine Sciences,2006,30(9):84-87.
• [6]李纯厚,王学锋,王晓伟,等.中国海水养殖环境质量及其生态修复技术研究进展[J].农业环境科学学报,2006,25(增刊):310-315.Li C H,Wang X F,Wang X W,et al.Water quality for mariculture and its ecological rehabilitation technology[J].Journal of Agro-Environment Science,2006,25(supp):310-315.
• [7]刘军,刘斌,谢骏.生物修复技术在水产养殖中的应用[J].水利渔业,2005,25(1):63-65.Liu J,Liu B,Xie J.Application of bioremediation technology in aquaculture[J].Water Conservancy and Fisheries,2005,25(1):63-65.
• [8] Bian G,Xu X X,Miao J Z,et al.Study on the process and characteristics of Co-immobilized ammonia-oxidizing bacteria and denitrifying bacteria[J].Hubei Agricultural Sciences,2016,55(1):170-175.
• [9] Yue J W,Wang P,Zhang Y P,et al.Study on entrapped immobilization and preservation of heterophic nitrification aerobic denitrify bacteria[J].Journal of Beijing Technology and Business University,2012,30(1):51-56.
• [10]商平,魏丽娜,刘涛利,等.改性沸石结合组合菌固定化小球去除河水中氨氮[J].水处理技术,2010,36(5):103-114.Shang P,Wei L N,Liu T L,et al.The immobilization pellet of modified zeolite binding combination bacteria removing ammonia nitrogen of river[J].Technology of Water Treatment,2010,36(5):51-56.
• [11]陈兵红.沸石固定化细胞处理农村生活污水中氨氮效果研究[J].环境科学与技术,2009,32(7):132-135.Chen B H.Biodegradation effect of ammonia nitrogen in rural domestic wastewater by immobilized cells with zeolite[J].Environmental Science and Technology,2009,32(7):132-135.
• [12]刘嘉夫,马越,谭盼.沸石联合固定化微生物技术抑制沉积物氨氮释放[J].科学技术与工程,2013,34(13):10232-10237.Liu J F,Ma Y,Tan P.Study on mechanism of removing ammonium from water by clinoptilolite-microbe combined immobilization technology[J].Science Technology and Engineering,2013,34(13):10232-10237.
• [13] Li W F,Fu L Q,Deng B,et al.Identification and denitrification characteristics of an aerobic denitrifier[J].Environmental Science,2011,32(8):2403-2408.
• [14]中国石油天然气总公司.SY/T 5162-1997岩石样品扫描电子显微镜分析方法[S].北京:中国标准出版社,1998.China National Petroleum Corporation.SY/T 5162-1997 Rock Sample Analysis by Scanning Electron Microscopy[S].Beijing:Standards Press of China,1998.
• [15]张闻,赵延君,王加宁,等.生物炭固定化石油降解菌剂的制备[J].江苏农业科学,2015,43(6):341-345.Zhang W,Zhao Y J,Wang J N,et al.Preparation of biochar immobilized petroleum degrading bacteria[J].Jiangsu Agricultural Sciences,2015,43(6):341-345.
• [16]周珊,周汇,单胜道.竹炭固定化微生物去除水样中氨氮的研究[J].林业科学,2009,45(6):133-138.Zhou S,Zhou H,Dan S D.Removal of ammonia nitrogen in aqueous samples by bamboo-carbon immobilized microorganism[J].Scientia Silvae Sinicae,2009,45(6):133-138.
• [17]张爽,姜蔚,徐桂芹,等.固定化硝化菌在不同温度下对氨氮的去除效能研究[J].环境科学与管理,2008,33(5):91-95.Zhang S,Jiang W,Xu G Q,et al.Study on the removal of nitrogen from wastewater using immobilized nitrobacteria at different temperature[J].Environmental Science and Management,2008,33(5):91-95.
• [18]李正魁,石鲁娜,杨竹攸,等.纯种氨氧化细菌Comamonas aquatic LNL3的固定化及短程硝化性能研究[J].环境科学,2009,30(10):2952-2957.Li Z K,Shi L N,Yang Z Y,et al.Immobilized ammonia-oxidizing bacteria Comamonas aquatic INl3and its partial nitrification characterization[J].Environmental Science,2009,30(10):2952-2957.
• [19] Dong Y,Zhang Y,Tu B.Immobilization of ammonia-oxidizing bacteria by polyvinyl alcohol and sodium alginate[J].Brazilian Journal of Micrbiology,2017,48:515-521.
• [20]袁林江.聚乙烯醇(PVA)固定化反硝化菌的脱氮特性[J].中国环境科学,1998,18(2):189-191.Yuan L J.Denitrification characters of denitrifying bacteria immobilized by polyvinyl alcohol(PVA)[J].China Environmental Science,1998,18(2):189-191.
• [21]辛福言,李秋芬,邹玉霞,等.虾池环境生物修复作用菌的模拟应用[J].应用与环境生物学报,2002,8(1):75-77.Xin F Y,Li Q F,Zou Y X,et al.Simulated application of functional bacteria in bioremediation of shrimp culture environment[J].Chinese Journal of Applied and Environmental Biology,2002,8(1):75-77.
• [22] N Maya E,Sanjoy B,Mohamed S,et al.Screening,identification and immobilization of ammonia oxidizing bacterial consortium collected from mangrove areas and shrimp farms[J].Asian Journal of Animal and Veterinary Advances,2013,8(1):73-81.
• [23] Chen J,Zhang A X,Zhong W H,et al.Ammonia removal of wastewater by immobilized ammonia-oxidizing bacteria with denitrying bacteria and biofilm observation[J].Biotechnology,2008,18(3):76-80.
• [24]罗华明,程岩雄,李利卫.养殖水质中亚硝酸盐、氨氮、硫化氢、pH值过高形成的原因、危害及处理办法[J].渔业致富指南,2005(14):34-34.Luo H M,Cheng Y X,Li L W.Cause,harm and treatment of nitrite,ammonia,hydrogen sulfide and high pH value in aquaculture water quality[J].A Guide to Enriching Fishery,2005(14):34-34.
• [25]黄正,范玮,李谷,等.固定化硝化细菌去除养殖废水中氨氮的研究[J].华中科技大学学报(医学版),2002,31(1):18-20.Huang Z,Fan W,Li G,et al.Removing ammonia nitrogen from fishery wastewater by immobilized nitrifying bacteria[J].Huazhong University of Science and Technology(Medical Edition),2002,31(1):18-20.
• [26]张晟瑀,林学钰,周兰影,等.沸石联合微生物固定化去除微污染水体中氨氮的研究[J].环境污染与防治,2009,4(31):14-17.Zhang S Y,Lin X Y,Zhou L Y,et al.Removing ammonia nitrogen fromslightly-polluted water by zeolite-microbe combined immobilization technology[J].Environmental Pollution and Prevention,2009,4(31):14-17.
• [27]田琳,孔强,仁宗明,等.活性炭和沸石对氨氮的吸附特性及生物再生[J].环境工程学报,2012,6(10):3424-3428.Tian L,Kong Q,Ren Z M,et al.Adsorption characteristics of activated carbon and zeolite and their biological regeneration[J].Chinese Journal of Environmental Engineering,2012,6(10):3424-3428.
• [28] Dong Y,Miao J Z,Chen A H.Research on immobilized ammonia-oxidizing bacteria by sodium alginate[J].Applied Mechanics and Materials,2014,618:283-287.
• [29] Liu S,Chu L,Zhu J.Removal of ammonia nitrogen from sewage by immobilization nitrification bacteria[J].Chinese Journal of Environmental Engineering,2014,8(10):4261-4266.

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