朱葆华,沈含,朱召霞,李赟,杨官品,潘克厚

• 1:海水养殖教育部重点实验室(中国海洋大学)
• 2:中国海洋大学海洋生命学院
• 3:青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室

摘要(Abstract)：

本实验采用添加硒的改良Zarrouk培养基培养钝顶螺旋藻(Arthrospira platensis)FACHB-834,测定了其叶绿素荧光参数、生物量及含碳率,探究硒对螺旋藻生长及固碳速率的影响。研究表明:较低的硒浓度(1和10 mg/L)促进螺旋藻生长,与对照组相比,生物量分别提高了21%和15%(P<0.05),终生物量最高达1.04 g/L;固碳速率最高达153.32 mg/(L·d);最大光能转换效率(F_v/F_m)、最大电子传递速率(rETR_(max))和光能的利用效率(α)与对照组相比均有所提高,但差异不显著。较高的硒浓度(50和100 mg/L)抑制螺旋藻的生长,与对照组相比生物量分别降低了43%和48%、含碳率分别降低了10%和17%、固碳速率分别降低了53%和61%(P<0.05),F_v/F_m分别最大降低了32.4%和31.2%、rETR_(max)分别最大降低了15.4%和11.2%、α值分别最大降低了48.2%和39.4%(P<0.05)。综合考虑钝顶螺旋藻的生长和固碳速率,本实验培养钝顶螺旋藻的最佳硒浓度为1 mg/L。研究结果可为大规模培养富硒钝顶螺旋藻提供参考。

关键词(KeyWords)： 钝顶螺旋藻;硒;生长;叶绿素荧光参数;固碳速率

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2016YFB0601001)资助~~

作者(Author): 朱葆华,沈含,朱召霞,李赟,杨官品,潘克厚

DOI: 10.16441/j.cnki.hdxb.20180223

参考文献(References)：

• [1] 师应来,胡晟明.技术进步、经济增长对二氧化碳排放的动态分析[J].统计与决策,2017(16):149-150.Ying S L,Hu C M.Dynamic analysis of carbon dioxide emissions by technological progress and economic growth[J].Statistics and Decision,2017(16):149-150.
• [2] 谢爽.利用污水污泥和高浓度二氧化碳培养海洋微藻技术研究[D].青岛:中国海洋大学,2010.Xie S.Study on the Cultivation Techniques of Marine Microalgae Using Sewage Sludge from Municipal Wastewater Treatment Plants Under High CO2 Concentrations[D].Qingdao:Ocean University of China,2010.
• [3] 杨玉娟.顿顶螺旋藻生长及固碳过程的影响因子研究[D].无锡:江南大学,2013.Yang Y J.The Study of Impact Factors Affecting the Growth and Carbon Fixation of Spirulina platensis[D].Wuxi:Jiang Nan University,2013.
• [4] Abanades J C,Alvarez D.Conversion limits in the reaction of CO2 with lime[J].Energy & Fuels,2003,17(2):308-315.
• [5] Kumar K,Dasgupta C N,Nayak B,et al.Development of suitable photobioreactors for CO2 sequestration addressing global warming using green algae and cyanobacteria[J].Bioresource Technology,2011,102(8):4945-4953.
• [6] Ho S H,Chen C Y,Lee D J,et al.Perspectives on microalgal CO2-emission mitigation systems-A review[J].Biotechnology Advances,2011,29(2):189-198.
• [7] Wang B,Li Y,Wu N,et al.CO2 bio-mitigation using microalgae[J].Applied Microbiology and Biotechnology,2008,79(5):707-718.
• [8] 福迪,罗迪安.藻类学[M].上海:上海科学技术出版社,1980.Fott B,Luo A D.Algal Science[M].Shanghai:Shanghai Science and Technology Press,1980.
• [9] Santillan C.Mass production of Spirulina[J].Cell Mol Life Sci,1982,38(1):40-43.
• [10] Becher E W.Production and utilization of the blue-green alga Spirulina in India[J].Biomas,1994,4:105-125.
• [11] Rotruck J T,Pope A L,Gantherhe,et al.Selenium:Biochemical role as a component of glutathione per-oxidase[J].Science,1973,179:588-590.
• [12] Rotruck J T,Banu L,Larsen P R.Type I iodothyronine deiodinase is a selenocysteine-containing enzyme [J].Nature,1991,349:438-440.
• [13] 徐晶,朱茂祥,陈婉华,等.螺旋藻富集同化无机硒研究[J].植物营养与肥料学报,1997,3(2):169-175.Xu J,Zhu M X,Chen W H,et al.Study on assimilation of Spirulina to inorganic Selenium[J].Plant Nutrition and Fertilizer Science,1997,3(2):169-175.
• [14] Julien C,Irena A W,Bertrand C,et al.Assessment of selenium bioavailability from high-selenium Spirulina subfraction in selenium-deficient rats[J].Journal of Agricultural and Food Chemistry,2002,50(13):3867-3873.
• [15] 杨芳,郑文杰,唐全民,等.Se(Ⅳ)对钝顶和极大螺旋藻生长的影响[J].暨南大学学报(自然科学版),2005,26(5):678-683.Yang F,Zheng W J,Tang Q M,et al.The effects of selenium(Ⅳ) on the growth of S.platensis and S.maxima[J].Journal of Jinan University(Natural Science),2005,26(5):678-683.
• [16] Chen T F,Zheng W J,Wong Y S,et al.Accumulation of selenium in mixotrophic culture of Spirulina platensis on glucose[J].Bioresource Technology,2007,97:2260-2265.
• [17] Huang Z,Zheng W J,Guo B J.Optimization of cultivation conditions in se-enriched Spirulina platensis[J].Chinese Journal of Biotechnology,2002,18(3):373-376.
• [18] 高海洋.LED光照对富锌和富硒螺旋藻生长的影响[D].湛江:广东海洋大学,2016.Gao H Y.Effect of LED on growth Zinc enrichment of Spirulina[J].Zhanjiang:Guangdong Ocean University,2016.
• [19] Wang D Z,Cheng Z D,Li S J,et al.Toxicity and accumulation of selenite in four microalgae[J].Chinese Journal of Oceanology and Limnology,2003,21(3):280-285.
• [20] Zhong Y,Chen T F,Zheng W J,et al.Selenium enhances antioxidant activity and photosynthesis in Ulva fasciata[J].Journal of Applied Phycology,2015,27(1):555-562.
• [21] 李志勇,郭祀远,李琳.富硒螺旋藻培养技术研究[J].水生生物学报,2001,25(4):386-391.Li Z Y,Guo S Y,Li L.A study of the se-rich cultivation technology of Spirulina platensis[J].Acta Hydrobiologica Sinica,2001,25(4):386-391.
• [22] 韩吉昌.产油微藻降耗、增效生产原理与技术研究[D].青岛:中国海洋大学,2017.Han J C.Biodiesel Production from Microalgae-Technical Research of Improving Efficiency and Reducing Cost[D].Qingdao:Ocean University of China,2017.
• [23] Zhu B,Sun F,Yang M,et al.Large-scale biodiesel production using flue gas from coal-fired power plants with Nannochloropsis microalgal biomass in open raceway ponds[J].Bioresource Technology,2014,174:53-59.
• [24] 朱葆华,常雅青,何文栋,等.RNA干扰UGP基因表达对三角褐指藻碳流分配的影响[J].中国海洋大学学报(自然科学版)2017,47(5):42-49.Zhu b H,Chang Y Q,He W D,et al.Effect of RNA interference of UGP-Glucose pyrophosphorylase gene expression on carbon allocation in Phaeodactylum tricornutum[J].Periodical of Ocean University of China:2017,47(5):42-49.
• [25] 陈新美,梅兴国,房伟,等.培养条件对螺旋藻生长和藻胆蛋白含量的影响[J].氨基酸和生物资源,2003(1):21-25.Chen X M,Mei X G,Fang W,et al.Effect of culture condition on growth of S.platensis and phycobiliprotein content[J].Amino Acids & Biotic Resources,2003,25(1):21-25.
• [26] 田辉,谯顺彬,彭晓东,等.无机与有机碳源混合营养培养对钝顶螺旋藻生长的影响[J].贵州农业科学,2011,39(10):122-124.Tian H,Qiao S B,Peng S D,et al.The influence of inorganic and organic carbon sources on mixtrophic growth of Spirulina platensis[J].Guizhou Agricultural Sciences,2011,39(10):122-124.
• [27] 段静波,刘文清,张玉钧,等.叶绿素荧光技术对受Cu2+胁迫藻类暗适应时间的研究[J].光子学报,2014,43(2):217002.Duan J B,Liu W Q,Zhang Y J,et al.Study on the dark adaptation time of algaes stressed by Cu2+ using chlorophyll fluorescence measurements[J].Acta Photonica Sinica,2014,43(2):217002.
• [28] Krall J P,Edwards G E.Environmental effects on the relationship between the quantum yields of carbon assimilation and in vivo PSII electron transport in maize[J].Australian Journal of Plant Physiology,1991,18(3):267-278.
• [29] 何晓艳,向文洲,何慧,等.海水钝顶螺旋藻富硒及其含硒藻蓝蛋白的研究[J].热带海洋学报,2005,24(4):30-34.He X Y,Xiang W Z,He H,et al.A study on Se accumulation and Se enriched phycocyanin in Spirulina platensis cultured in seawater-based medium[J].Journal of Tropical Oceanography,2005,24(4):30-34.
• [30] Li Z Y,Guo S Y,Li L.Bioeffects of selenite on the growth of Spirulina platensis and its biotransformation[J].Bioresour Technol,2003,89(2):171-176.
• [31] Cepoi L,Zinicovscaia I,Zosim L,et al.Metals Removal by Cyanobacteria and Accumulation in Biomass[M].Switzerland:Springer International Publishing,2016.
• [32] 周志刚,尹长松.富硒极大螺旋藻整细胞、藻胆体及藻蓝蛋白的光谱特性[J].上海水产大学学报,2002,11(3):205-214.Zhou Z G,Yin C S.Spectrum properties of intact cells,phycobilisomes and phycoyanin from se-rich Spirulina maxima[J].Journal of Shanghai Fisheries University,2002,11(3):205-214.
• [33] Feng T,Chen S S,Gao D Q,et al.Selenium improves photosynthesis and protects photosystem II in pear (Pyrus bretschneideri),grape (Vitis vinifera),and peach (Prunus persica)[J].Photosynthetica,2015,53(4):609-612.
• [34] Zhang M,Tang S,Huang X,et al.Selenium uptake,dynamic changes in selenium content and its influence on photosynthesis and chlorophyll fluorescence in rice (Oryza sativa L.)[J].Environ and Experimental Botany,2014,107:39-45.
• [35] Kreft I,Mechora ?,Germ M,et al.Impact of selenium on mitochondrial activity in young tartary buckwheat plants[J].Plant Physiology & Biochemistry Ppb,2013,63(4):196-199.
• [36] 单雪凤,张水妹,蔡智辉,等.富硒螺旋藻光谱特性及其含硒蛋白质组分析[J].分析化学,2016,44(12):1814-1819.Shan X F,Zhang S M,Cai Z H.Infrared spectrum,UV-visible absorption spectral characteristics and proteomic analysis of selenium enriched Spirulina platensis[J].Chinese Journal of Analytical Chemistry,2016,44(12):1814-1819.
• [37] Glazer A N,Yeh S N,Webb S P.Disk-to-disk transfer as the rate-limiting step for energy flow in phycobilisomes[J].Science,1985,227:419-423.
• [38] 马文彦.硒(IV)、碲(IV)双组分胁迫对螺旋藻的生理生化影响[D].广州:暨南大学,2006.Ma W Y.Effects of Se(IV)、Te(IV) Stress on the Growth of Spiurlina[D].Guangzhou:Jinan University,2006.
• [39] Othman A I,El Missiry M A.Role of selenium against lead toxicity in male rats[J].Journal of Biochemical & Molecular Toxicology,2015,12(6):345-349.
• [40] 尹希.硒对钝顶螺旋藻和人血红细胞氧化损伤的保护作用及其机制[D].广州:暨南大学,2010.Yin X.Protection and Mechanisms of Selenium on Spirulina platensis and Human Red Blood Cells Against Oxidative Stress[D].Guangzhou:Jinan University,2010.
• [41] 乔玉辉,商树田.施硒对钝顶螺旋藻(S.platensis)品质的影响[J].中国农业大学学报,2000,5(1):31-34.Qiao Y H,Shang S T.Effects of selenium (Se) on quality of Spirulina platensis(Sp.-D)[J].Journal of China Agricultural University,2000,5(1):31-34.
• [42] Bhat V B,Madyastha K M.C-Phycocyanin:A potent peroxyl radical scavenger in vivo,and in vitro[J].Biochemical & Biophysical Research Communications,2000,275(1):20-25.
• [43] 尹长松.硒对极大螺旋藻整细胞、藻胆体及藻蓝蛋白光谱特性的影响[D].上海:上海水产大学,2002.Yin C S.Effects of selenium on spectra of intact cells、phycobilisomes and phycocyanin from Spirulina maxima[D].Shanghai:Shanghai Ocean University,2002.
• [44] Imlay J A,Linn S.DNA damage and oxygen radical toxicity[J].Science,1998,240:1302-1309.
• [45] Ray N R,Ray A K.Liver succinoxidase and kidney succinic dehydrogenase activities on selenium toxicity[J].Indian Veterinary Journal,1975,52:267-270.
• [46] Padmaja K,Somasekharaiah B V,Prasad A R K.Inhibition of chlorophyll synthesis by selenium:Involvement of lipoxygenase mediated lipid peroxidation and antioxidant enzymes[J].Photosynthetica,1995,31:1-7.

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