王玉明,杜磊,周鑫,任兵兴,王静凤,薛长湖

• 1:中国海洋大学食品科学与工程学院

摘要(Abstract)：

本研究采用添加10%不同水产品蛋白的标准饲料或高胆固醇饲料喂食小鼠,研究高胆固醇水产品对小鼠脂质代谢的影响。通过测定小鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-c)的浓度以及肝脏TC、TG和磷脂(PL)的含量,同时称量小鼠体质量、肝脏、肾脏重量以及肾周脂肪和精巢上部脂肪重量,以评价不同蛋白质对小鼠成长的影响,并收集了高胆固醇血症模型小鼠的排泄物并测定其总脂质和中性固醇的排泄量。结果显示,水产品作为优质蛋白质能明显降低正常小鼠和高胆固醇血症模型小鼠的血清总脂水平(P<0.05)以及动脉粥样硬化指数(P<0.05),不同水产品对小鼠肝脂水平及高胆固醇血症小鼠排泄物中总脂质和中性固醇的排泄量的影响因水产品种类而不同。提示本研究中的高胆固醇水产品对由高胆固醇血症引发的动脉粥样硬化不具有危害性。

关键词(KeyWords)： 水产品;胆固醇;甘油三酯;动脉粥样硬化

Abstract:

Keywords:

基金项目(Foundation): 国际科技合作项目(2010DFA31330)资助

作者(Author): 王玉明,杜磊,周鑫,任兵兴,王静凤,薛长湖

DOI: 10.16441/j.cnki.hdxb.2011.05.005

参考文献(References)：

• [1]Ozawa A,Aoki S,Suzuki K,et al.Taurine content in fish andshells[J].Nippon Eiyo Shokuryo Gakkaishi,1984,37(6):561-567.
• [2]李丽莉.几种海产品中氨基酸及牛磺酸含量的比较[J].氨基酸和生物资源,1999,21(2):25-26.
• [3]Durrington P N,Bhatnagar D,Mackness MI,et al.An omega-3polyunsaturated fatty acid concentrate administered for one yeardecreased triglycerides in si mvastatin treated patients with coro-nary heart disease and persisting hypertriglyceridaemia[J].Heart,2001,85:544-548.
• [4]Ties F,Garry J M,Yaqoob P,et al.Association of n-3 polyunsat-urated fatty acids with stability of atherosclerotic plaques:a ran-domised controlled trial[J].Lancet,2003,361:477-485.
• [5]Marthal D,Jeremiahs T,Anthonyj O,et al.Fish consumptionand the 30-year risk of fatal myocardial infarction[J].The NewEngland Journal of Medicine,1997,336(15):1046-1053.
• [6]Marian T,Carol S,Irena B,et al.Effects of shellfish consump-tion on lipoproteins in normolipidemic men[J].AmJ Clin Nutr,l990,51:1020-1027.
• [7]Folch J,Lees M,Sloane-stanley G H.Americaninstitute of nutri-tion:Report of the Americaninstitute of nutrition ad hoc commit-tee on standards for nutritional studies[J].J Nutr,1977,107:1340-1348.
• [8]Hayakawa K,Mishi ma K,Nozako M,et al.High-cholesterolfeeding aggravates cerebral infarction via decreasingthe CB1recep-tor[J].Neuroscience Letters,2007,414(6):183-187.
• [9]Hai Lin Zhao,Kyung Hyun C,Young W,et al.Cholesterol-low-ering effect of platycodin Din hypercholesterolemic ICR mice[J].European Journal of Pharmacology,2006,537:166-173.
• [10]Folch J ML,Slane-stanley G H.Asi mple method for the isola-tion and purification of total lipids fromani mal tissues[J].J BiolChem,1957,226(1):497-506.
• [11]GB11893-89:水中总磷的测定,钼酸铵分光光度法[S].北京:中国标准出版社,1991:120-121.
• [12]Tanaka K,Sakai T,Ikeda I,et al.Effects of dietary shri mp,squid and octopus on serumandliver lipidlevelsin mice[J].Bio-sci Biotechnol Biochem,1998,62(7):1369-1375.
• [13]Bobek P,Ozdín L,Kuniak L,et al.Regulation of cholesterolmetabolism with dietary addition of oyster mushrooms(Pleurotusostreatus)in rats with hypercholesterolemia[J].Cas Lek Cesk,1997,136(6):186-190.
• [14]Hidehiko Y,Hideki M,Ken N,et al.Dietary taurine enhancescholesterol degradation and reduces serum and liver cholesterolconcentrations in rats fed a high-cholesterol diet[J].Journal ofNutrition,1999,129:1705-1712.
• [15]Taesun P,Kyungshin L,Youngsook Um.Dietary taurine sup-plementation reduces plasma andliver cholesterol and triglycerideconcentrations in rats fed a high-cholesterol diet[J].NutritionResearch,1998,18(9):1559-1571.
• [16]陈世伟,张丁,刘翠娥,等.L-肉碱对高脂膳食诱导肥胖大鼠体脂含量及血脂水平的影响[J].预防医学文献信息,2003,9(4):385-389.
• [17]Ioannis I,Irini M,Marc R,et al.L-Carnitine and acetyl-L-carni-tine in the treat ment of complications associated with HI Vinfec-tion and antiretroviral therapy[J].Mitochondrion,2004,4:163-168.
• [18]Sugiyama K,Muramatsu K.Effects of methionine and relatedcompounds on plasma cholesterol level in rats fed a high choles-terol diet[J].Nutr Sci Vitaminol,1986,32:537-547.
• [19]Virtanen E,Rumsey G.Betaine supplementation can opti mizeuse of methionine,choline in diets[J].Feedstuffs,1996,68(42):12-13.
• [20]Tanaka K,Ikuo I,Hiroko Y,et al.Effects of dietary defattedsquid on cholesterol metabolism and hepatic lipogenesis in rats[J].Lipids,2001,36(5):461-466.
• [21]Bergeron N,Jacques H.Influence of fish protein as compared tocasein and soy protein on serumand liver lipids,and serumlipo-protein cholesterol levels in the rabbit[J].Atherosclerosis,1989,78(2-3):113-121.

文章评论(Comment)：