何峰,马瑞芹,赵旭,张宇,于锦涛,温海深,李吉方

• 1:中国海洋大学海水养殖教育部重点实验室

摘要(Abstract)：

芳香化酶基因(CYP19)编码的芳香化酶是雄激素转化为雌激素的关键酶,在鱼类性腺发育及繁殖内分泌过程中起着重要的作用。本研究采用聚合酶链式反应-单链构象多态(PCR-SSCP)和放射性免疫方法(RAI)对61尾雌牙鲆进行了CYP19A基因启动子CpG岛区多态性检测,并将其与繁殖性能进行了关联分析。CpG岛区存在3种类型的基因型(pattern1、pattern2、pattern3),经测序发现CpG岛区有以下突变:T-1927插入或缺失、C-1899插入或缺失、T-1772C、C-1766T、TGTCAAC-1752~-1744插入或缺失(11个SNPs连锁突变)。方差分析结果表明:此位点对雌二醇(E2)水平的效应达到显著(P<0.05)水平,经多重比较分析,具有pattern1基因型的个体的雌激素(E2)水平显著低于pattern2和pattern3基因型个体的雌激素水平(P<0.05),同时pattern1基因型的个体的mRNA表达量显著低于pattern2和pattern3基因型个体的mRNA表达量(P<0.05)。以上结果表明,CYP19A基因调控区甲基化位点突变可能导致芳香化酶的表达量,进而影响牙鲆繁殖内分泌过程;同时也阐明了这些与牙鲆繁殖性状相关的SNPs可以用于生产实践中进行标记辅助选择具有优良繁殖性能的牙鲆。

关键词(KeyWords)： 牙鲆;CYP19A基因;SNPs;繁殖性能

Abstract:

Keywords:

基金项目(Foundation): 教育部博士点新教师基金项目(20090132120006);; 国家大学生创新性实验项目(091042331);; 山东省自然科学基金项目(ZR2009DQ011);; 山东省博士后创新基金项目(200702039)资助

作者(Author): 何峰,马瑞芹,赵旭,张宇,于锦涛,温海深,李吉方

DOI: 10.16441/j.cnki.hdxb.2011.09.003

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