梁英,孟祥荣,范丽敏,田传远,黄徐林

• 1:海水养殖教育部重点实验室(中国海洋大学)

摘要(Abstract)：

本文研究了0.1~5mg/L的3-吲哚乙酸(IAA)对球等鞭金藻(Isochrysis galbana)和0.1~3mg/L的IAA对棕鞭藻(Ochromonas neopolitana)的叶绿素荧光参数(PSII最大光能转化效率Fv/Fm、相对电子传递速率rETR、光化学淬灭qP)、细胞密度、叶绿素含量、总脂含量及脂肪酸组成的影响。研究表明,球等鞭金藻和棕鞭藻进行光合作用和生长的最适IAA浓度分别为0.1和0.25mg/L。此条件下,2株藻的Fv/Fm、rETR、qP、细胞密度和叶绿素含量均显著高于对照组和其它处理组。其中,球等鞭金藻的细胞密度和叶绿素含量分别比对照组增加了32.6%和37.1%,总脂含量显著高于其它处理组,但与对照组差异不显著(P>0.05);棕鞭藻的细胞密度和叶绿素含量在IAA浓度为0.25mg/L时分别比对照组增加了48.7%和34.4%,总脂含量在IAA浓度为0.5mg/L时显著高于对照组和其它处理组,但与0.25mg/L处理组差异不显著(P>0.05)。0.1mg/L的IAA浓度对球等鞭金藻DHA(二十二碳六烯酸)的合成有促进作用,但对其它各脂肪酸(14:0、16:0、16:1n-7和18:1n-9)含量均无显著影响;0.25mg/L的IAA浓度促进了棕鞭藻DHA和多不饱和脂肪酸的合成。研究结果表明,球等鞭金藻和棕鞭藻进行生长和油脂积累的最适IAA浓度分别为0.1和0.25mg/L,此结果为2株藻的大规模培养提供了理论依据。

关键词(KeyWords)： 球等鞭金藻;棕鞭藻;3-吲哚乙酸(IAA);生长;叶绿素荧光参数;总脂含量;脂肪酸

Abstract:

Keywords:

基金项目(Foundation): “十二五”国家科技支撑计划项目(2011BAD14B01);; 国家高技术研究发展计划项目(2014AA022001)资助~~

作者(Author): 梁英,孟祥荣,范丽敏,田传远,黄徐林

DOI: 10.16441/j.cnki.hdxb.20170166

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