杨慧,陈吉祥,公衍军,李彩风,李筠,杨官品

• 1:中国海洋大学海洋生命学院
• 2:中国海洋大学海洋生命学院
• 3:中国海洋大学海洋生命学院
• 4:中国海洋大学海洋生命学院

摘要(Abstract)：

从致病性鳗弧菌W-1基因组DNA扩增并克隆了1 156bp的特异性片段,含有完整的外膜蛋白ompU基因阅读框,由993个核苷酸组成,编码330个氨基酸残基的蛋白质。与国外已发表的鳗弧菌外膜蛋白基因的序列同源性为100%,与创伤弧菌(Vibrio vulnificus)、霍乱弧菌(Vibrio cholerae)、费氏弧菌(Vibrio fischeri)和副溶血弧菌(Vibrio parahaemolyticus)的序列同源性分别为72%,69%,68%和64%。将该外膜蛋白基因克隆于pBV220表达质粒,在大肠杆菌中得到了表达,SDS-PAGE分析表明表达蛋白分子量约38kDa,Western blot分析发现表达蛋白能与鳗弧菌外膜蛋白抗体很好的反应。

关键词(KeyWords)： 鳗弧菌;外膜蛋白;ompU基因;克隆;表达

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30371108);; 国家高技术研究发展计划项目(2003AA622070)资助

作者(Author): 杨慧,陈吉祥,公衍军,李彩风,李筠,杨官品

DOI: 10.16441/j.cnki.hdxb.2006.s1.020

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