池振明,马再超

• 1:中国海洋大学海洋生命学院

摘要(Abstract)：

从葡萄糖阻遏作用、铁离子引起的阻遏作用、氮阻遏作用和诱导机理几个方面对酵母菌中诱导和阻遏的分子机理进行了综述。目前研究认为,在葡萄糖阻遏作用方面,Mig1蛋白和Snf1蛋白参与了葡萄糖阻遏,并且磷脂酰肌醇类的信号传导对葡萄糖阻遏起着重要作用;在铁离子引起的阻遏作用方面,GATA蛋白能够有效阻遏铁载体的生物合成;在氮阻遏作用方面,Gln3、Gat1、Dal80和Deh1是参与氮阻遏的4个调节蛋白;而在酵母菌诱导过程中,转录激活蛋白起着非常重要的作用。

关键词(KeyWords)： 酵母菌;诱导;阻遏;分子机理

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31070029)资助

作者(Author): 池振明,马再超

DOI: 10.16441/j.cnki.hdxb.2013.01.007

参考文献(References)：

• [1]沈萍,陈向东.微生物学[M].北京:高等教育出版社,2006:238-243.
• [2]Gancedo J M.Yeast carbon catabolite repression[J].Microbiolo-gy and Molecular Biology Reviews,1998,62:334-361.
• [3]Hofman-Bang J.Nitrogen catabolite repression in Saccharomycescerevisiae[J].Molecular Biotechnology,1999,12:35-73.
• [4]Coffman J A,Rai R,Cunningham T,et al.Gat1p,a GATA fam-ily protein whose production is sensitive to nitrogen catabolite re-pression,participates in transcriptional activation of nitrogencata-bolicgenes in Saccharomyces cerevisiae[J].Molecular and CellularBiology,1996,16:847-858.
• [5]Coffman J A,Rai R,Loprete D M,et al.Cross regulation of fourGATA factors that control nitrogen catabolic gene expression inSaccharomyces cerevisiae[J].Journal of Bacteriology,1997,179:3416-3429.
• [6]Cooper T G.Transmitting the signal of excess nitrogen in Saccha-romyces cerevisiae from the Tor proteins to the GATA factors:connecting the dots[J].FEMS Microbiology Reviews,2002,26:223-238.
• [7]Courchesne W E,Magasanik B.Regulation of nitrogen assimila-tion in Saccharomyces cerevisiae:roles of the URE2and GLN3genes[J].Journal of Bacteriology,1988,170:708-713.
• [8]Cox K H,Rai R,Distler M,et al.Saccharomyces cerevisiae GA-TA sequences function as TATA elements during nitrogen catabo-lite repression and when Gln3p is excluded from the nucleus by o-verproduction of Ure2p[J].Journal of Biological Chemistry,2000,275:17611-17618.
• [9]Cunningham T S,Andhare R,Cooper T G.Nitrogen cataboliterepression of DAL80expression depends on the relative levels ofGat1p and Ure2p production in Saccharomyces cerevisiae[J].Journal of Biological Chemistry,2000,275:14408-14414.
• [10]Cunningham T S,Cooper T G.Expression of the DAL80gene,whose product is homologous to the GATA factors and is a nega-tive regulator of multiple nitrogen catabolic genes in Saccharomy-ces cerevisiae,is sensitive to nitrogen catabolite repression[J].Molecular and Cellular Biology,1991,11:6205-6215.
• [11]Cunningham T S,Cooper T G.The Saccharomyces cerevisiaeDAL80repressor protein binds to multiple copies of GATAA-containing sequences(URSGATA)[J].Journal of Bacteriology,1993,175:5851-5861.
• [12]Cunningham T S,Dorrington R A,Cooper T G.The UGA4UASNTRsite required for GLN3-dependent transcriptional activa-tion also mediates DAL80-responsive regulation and DAL80pro-tein binding in Saccharomyces cerevisiae[J].Journal of Bacteri-ology,1994,176:4718-4725.
• [13]Cunningham T S,Rai R,Cooper T G.The level of DAL80ex-pression down-regulates GATA factor-mediated transcription inSaccharomyces cerevisiae[J].Journal of Bacteriology,2000,182:6584-6591.
• [14]Deberardinis R J,Lum J J,Hatzivassiliou G,et al.The biologyof cancer:metabolic reprogramming fuels cell growth and prolif-eration[J].Cell Metabolism,2008,7:11-20.
• [15]Feller A,Boeckstaens M,Marini A M,et al.Transduction ofthe nitrogen signal activating Gln3-mediated transcription is inde-pendent of Npr1kinase and Rsp5-Bul1/2ubiquitin ligase in Sac-charomyces cerevisiae[J].Journal of Biological Chemistry,2006,281:28546-28554.
• [16]Chi Z M,Li J F,Wang X H,et al.Inositol and phosphatidyli-nositol mediated glucose derepression,gene expression and in-vertase secretion in yeasts[J].Acta Biochimica et Biophysica Sin-ica,2004,36:443 449.
• [17]Yao S,Chi Z M,He S.Studies on inositol-mediated expressionof MAL gene encoding maltase and phospholipid biosynthesis inSchizosaccharomyces pombe[J].Journal of Industrial Microbiol-ogy and Biotechnology,2006,33:417-422.
• [18]Yao S,Chi Z M,He S.Regulation of MAL1+gene expressionencoding maltase in Schizosaccharomyces pombe by added inositol[J].Indian Journal of Biochemistry and Biophysics,2006,43:143-147
• [19]Chi Z,Ma L,Gao L,et al.Added inositol regulates invertase se-cretion and glucose-repressed SUC2gene expression in Saccharo-myces sp.W4[J].Indian Journal of Biochemistry and Biophys-ics,2007,44(3):106-113.
• [20]Chi Z M,Ni X,Yao S.Cloning and overexpression of a maltasegene from Schizosaccharomyces pombe in Escherichia coli andcharacterization of the recombinant maltase[J].Mycol Res,2008,112:983-989.
• [21]Chi Z M,He S,Yao S.Effects of Pichia pastoris INO1expres-sion in Schizosaccharomyces pombe on phosphatidylinositol(PI)synthesis and expression of INV+encoding invertase[J].En-zyme and Microbial Technology,2005,37:395-401.
• [22]Chi Z M,Chi Z,Liu G,et al.Saccharomycopsis fibuligera andits applications in biotechnology[J].Biotechnology Advances,2009,27:423-431.
• [23]Cassart J P,Ostling J,Ronne H,et al.Comparative analysis inthree fungi reveals structurally and functionally conserved regionsin the Mig1repressor[J].Molecular and General Genetics,1997,255:9-18.
• [24]Liu G L,Wang D S,Wang L F,et al.Mig1is involved in myce-lial formation and expression of the genes encoding extracellularenzymes in Saccharomycopsis fibuligera A11[J].Fungal Genet-ics and Biology,2011,48:904-913.
• [25]Lachance M A.Kluyveromyces van der Walt em end.van derWalt[M].Netherlands:Amsterdam,Elsevier,1998:227-247.
• [26]Llorente B,Malpertuy A,Blandin G,et al Genomic explorationof the hemiascomycetous yeasts,12.Kluyveromyces marxianusvar.marxianus[J].FEBS Letters,2000,487:71-75.
• [27]Lane M M,Morrissey J P.Kluyveromyces marxianus:A yeastemerging from its sister′s shadow[J].Fungal Biology Reviews,2010,24(2):1-10.
• [28]Haas H.Molecular genetics of fungal siderophore biosynthesis anduptake:the role of siderophores in iron uptake and storage[J].Ap-plied Microbiology and Biotechnology,2003,62:316-330.
• [29]Hider R C,Kong X L.Chemistry and biology of siderophores[J].Natural Product Reports,2010,27:637-657.
• [30]Scazzocchio C.The fungal GATA factors[J].Current OpinionIn Microbiology,2000,3(2):126-131.
• [31]Harrison K A,Marzluf G A.Characterization of DNA bindingand the cysteine rich region of SRE,a GATA factor in Neurospo-ra crassa involved in siderophore synthesis[J].Biochemistry,2002,41:15288-15295.
• [32]An Z,Mei B,Yuan W M,et al.The distal GATA sequences ofthe sid1promoter of Ustilago maydis mediate iron repression ofsiderophore production and interact directly with Urbs1,a GATAfamily transcription factor[J].EMBO Journal,1997,16:1742-1750.
• [33]Zhou L,Marzluf G A.Functional analysis of the two zinc fingersof SRE,a GATA-type factor that negatively regulates sid-erophore synthesis in Neurospora crassa[J].Biochemistry,1999,38:4335-4341.
• [34]Renshaw J C,Robson G D,Trinci A P J,et al.Fungal sid-erophores:structures,functions and applications[J].Mycologi-cal Research,2002,106:1123-1142.
• [35]Oberegger H,Schoeser M,Zadra I,et al.SREA is involved inregulation of siderophore biosynthesis,utilization and uptake inAspergillus nidulans[J].Molecular Microbiology,2001,41:1077-1089.
• [36]Martins L J,Jensen L T,Simon J R,et al.Metalloregulation ofFRE1and FRE2 homologs in Saccharomyces cerevisiae[J].Journal of Biological Chemistry,1998,273:23716-23721.
• [37]Wang W,Chi Z,Liu G,et al.Chemical and biological character-ization of siderophore produced by the marine-derived Aureobasi-dium pullulans HN6.2and its antibacterial activity[J].Biomet-als,2009,22:965-972.
• [38]Wang W L,Chi Z M,Chi Z,et al.Siderophore production bythe marine-derived Aureobasidium pullulans and its antimicrobialactivity[J].Bioresource Technology,2009,100:2639-2641.
• [39]Chi Z,Wang X X,Ma Z C,et al.The unique role of siderophorein marine-derived Aureobasidium pullulans HN6.2[J].Biomet-als,2012,25:219-230.
• [40]Johnston M,Flick J S,Pexton T.Multiple mechanisms providerapid and stringent glucose repression of GAL gene expression inSaccharomyces cerevisiae[J].Molecular and Cellular Biology,1994,14,3834-3841.
• [41]Li J,Wang S,VanDusen W J,et al.Green fluorescent protein inSaccharomyces cerevisiae:real-time studies of the GAL1promot-er[J].Biotechnology and Bioengineering,2000,70:187-196.
• [42]Rohde J R,Trinh Sadowski.Multiple signals regulate GAL tran-scription in yeast[J].Molecular and Cellular Biology,2000,20(11):3880-3886.
• [43]Vodala S,Abruzzi K C,Rosbash M.The nuclear exosome andadenylation regulate posttranscriptional tethering of yeast GALgenes to the nuclear periphery[J].Molecular Cell,2008,31:104-113.
• [44]Luthra R,Kerr S C,Harreman M T,et al.Actively transcribedGAL genes can be physically linked to the nuclear pore by theSAGA chromatin modifying complex[J].Journal of BiologicalChemistry,2007,282:3042-3049.
• [45]Kumar P R,Yu Y,Sternglanz R,et al.NADP regulates theyeast GAL induction system[J].Science,2008,319:1090-1092.

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