王彩霞,吴绍强,林祥梅,王素华,冯春燕,徐赓

• 1:中国检验检疫科学研究院动物检疫研究所
• 2:浙江省温州出入境检验检疫局
• 3:山东省沂南县畜牧兽医局

摘要(Abstract)：

以焦磷酸测序技术为检测平台,在研究包纳米虫基因特性的基础上,选择包纳米虫(Bonamiaspp.)DNA序列的保守区域,利用焦磷酸测序软件设计专用引物。以OIE推荐的PCR方法构建的阳性质粒作为模板,建立了包纳米虫的PCR-焦磷酸测序检测方法,确定测得序列为包纳米虫序列,经酶切位点分析可鉴别感染种类。以建立的包纳米虫PCR-焦磷酸测序方法对进口牡蛎(Ostrea gigas thunberg)样品进行检测,同时以OIE推荐的PCR-RFLP方法进行对照检测,检测结果表明,本研究建立的PCR-焦磷酸测序检测方法可以在基因序列水平上准确鉴定样品是否为包纳米虫感染以及感染种类,检测结果与OIE推荐方法的检测结果一致。研究结果表明,本研究建立的检测方法可应用于口岸包纳米虫感染情况的检测鉴定。

关键词(KeyWords)： 牡蛎;包纳米虫;聚合酶链式反应;焦磷酸测序;检测;鉴定

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑计划项目(2013BAD12B02)资助~~

作者(Author): 王彩霞,吴绍强,林祥梅,王素华,冯春燕,徐赓

DOI: 10.16441/j.cnki.hdxb.20160408

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