杨国宁,陈列欢,曾毅伟,刘万顺

• 1:中国海洋大学海洋生命学院
• 2:淮海工学院海洋学院
• 3:江苏省海洋资源开发研究院甲壳素研发中心

摘要(Abstract)：

建立了聚合度2～10的壳寡糖的薄层层析分析(TLC)方法,在异丙醇∶水∶氨水=60∶30∶4的展开体系中,各壳寡糖可以得到很好的分离,各单体间比移值(Rf)差异明显,无拖尾现象。通过对比TLC法与高效液相色谱(HPLC)法分析壳寡糖单体的效果,得出TLC法具有快速、有效、方便的优点,可以作为定性分析壳寡糖单体纯度的方法。采用本实验室制备的壳聚糖酶对3种不同脱乙酰度的壳聚糖和DP2～6的壳寡糖进行水解,采用TLC法对水解产物进行分析。实验结果表明,随着壳聚糖脱乙酰度的降低,其酶水解产物的糖链延长。该壳聚糖酶不能水解DP2～4的壳寡糖,对DP5～6的壳寡糖水解速率缓慢。通过对该壳聚糖酶作用特点分析,表明该酶可作用于GlcN-GlcN糖苷键,而不能水解Glc-NAc-GlcNAc糖苷键。

关键词(KeyWords)： 壳寡糖;壳聚糖酶;薄层色谱;高效液相色谱

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 杨国宁,陈列欢,曾毅伟,刘万顺

DOI: 10.16441/j.cnki.hdxb.2013.02.004

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