周晓苏,王旭波,宋华玉,王志刚,于海洋,齐洁,姜海滨,张全启

• 1:中国海洋大学海洋生命学院海洋生物遗传学与育种教育部重点实验室
• 2:山东省海洋水产研究所

摘要(Abstract)：

促性腺激素释放激素(GnRH)是1种在下丘脑-垂体-性腺轴(Hypothalamus-pituitary-gonad,HPG)的活动中发挥关键作用的神经激素。研究采用染色体步移方法,从许氏平鲉的肌肉组织中克隆得到GnRH1、GnRH2和GnRH3共3个基因的启动子序列,长度分别为4,3.5和2.5kb。利用在线生物信息学软件对这3个GnRH基因的启动子进行预测分析,结果表明,3个基因均存在Sp1,Oct-1,C/EBP,CREB,Pit-1,NF-1,Brn,GATA-1,USF和MyoD等转录因子的结合位点,以及糖皮质激素受体(GR),雄激素受体(AR),雌激素受体(ER),黄体酮受体(PR),视黄酸受体(RAR)和视黄醇受体(RXR)等核受体的结合位点,推测其对GnRH的调控起到关键作用。但是甲状腺激素受体(TR)和嗅觉相关的Olf-1的结合位点只在GnRH1和GnRH2基因启动子中发现,而在GnRH3中没有预测出。本研究结果表明许氏平鮋不同类型的GnRH是受不同的机制调控的,从而在生理过程中发挥着不同的作用。

关键词(KeyWords)： 许氏平鲉;促性腺激素释放激素;启动子;生物信息学分析

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展计划项目(2012AA10A401);; “国家科技基础条件平台-水产种质”项目资助

作者(Author): 周晓苏,王旭波,宋华玉,王志刚,于海洋,齐洁,姜海滨,张全启

DOI: 10.16441/j.cnki.hdxb.2012.10.009

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