谢聪颖,钟雪峰,乌兰,张馨予,田霖,杜国英

• 1:中国海洋大学海洋遗传与育种教育部重点实验室

摘要(Abstract)：

为探讨在低温胁迫下条斑紫菜(Neopyropia yezoensis)的生长及光合生理生化响应，本实验以条斑紫菜叶状体为研究对象，测定2℃低温处理条件下条斑紫菜的相关光合生理指标、光合色素含量以及日生长率。研究表明，条斑紫菜叶状体在低温胁迫下生长变缓，野生样品在短期(4 d)胁迫下日生长率(DGR)为1.59%·d~(-1)～3.77%·d~(-1),长期(20 d)胁迫后DGR为2.19%·d~(-1)～4.12%·d~(-1);苗期条斑紫菜在低温胁迫(2℃)前2 d时DGR为3.75%·d~(-1),处理4 d时DGR为8.79%·d~(-1),经过6 d胁迫后DGR为7.19%·d~(-1)。此外在低温胁迫下条斑紫菜的光合能力也减弱，野生样品在短期(4 d)低温胁迫下，F_v/F_m(PSⅡ最大光化学量子产量)、Φ_(PSⅡ)(PSⅡ实际光化学量子产量)和qP(光化学淬灭系数)均降低，NPQ(非光化学淬灭系数)则升高；经过长期(20 d)胁迫后，F_v/F_m仍显著降低，Φ_(PSⅡ)和qP上升，NPQ降至最低。苗期条斑紫菜在低温胁迫下前2 d F_v/F_m、Φ_(PSⅡ)、NPQ和qP均降低，胁迫4 d时F_v/F_m、Φ_(PSⅡ)、NPQ和qP上升，胁迫6 d时各荧光参数与4 d时相比无显著变化。苗期条斑紫菜低温胁迫6 d后，叶绿素a、藻蓝蛋白含量显著下降(P<0.05),藻红蛋白含量极显著下降(P<0.01),别藻蓝蛋白含量极显著上升(P<0.01)。研究结果表明，条斑紫菜在光合生理及生化上响应低温胁迫并最终影响其生长速率，且随着低温胁迫时间的延长表现出一定的适应性。本研究为探讨条斑紫菜对低温胁迫的适应机制和筛选耐低温良种提供数据参考。

关键词(KeyWords)： 条斑紫菜;低温胁迫;光合生理;生长

Abstract:

Keywords:

基金项目(Foundation): 国家重点研究发展计划项目(2022YFD2400105,2020YFD0900702)资助~~

作者(Author): 谢聪颖,钟雪峰,乌兰,张馨予,田霖,杜国英

DOI: 10.16441/j.cnki.hdxb.20230169

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