王浩东,姚雪,池姗,赵翠,金月梅,刘翠,朱明壮,刘涛

• 1:中国海洋大学海洋生命学院
• 2:中国海洋大学水产学院

摘要(Abstract)：

通过生殖细胞观察以及DNA含量检测研究南黄海浒苔(Ulva prolifera)群体的世代结构,发现近岸和近海漂浮浒苔群体均由孢子体世代和配子体世代构成,呈现混合世代群体结构。利用完全随机实验设计研究温度、光照强度和盐度对浒苔孢子体和配子体生殖的影响,发现3个生态因子对浒苔配子体和孢子体生殖的影响存在交互效应,在温度25℃、盐度40和光照7 000lx条件下,在24～48h内可促进配子体和孢子体的生殖发育;较高温度(25℃)和较低盐度(10)处理对配子的放散有极显著影响,而较高温度(25℃)和较高盐度(40)处理对孢子的放散有极显著影响。配子体和孢子体的生殖发育对基本环境因子存在着一定的差异反应,但两者均可在较宽的温度和盐度范围内生殖,这可能是导致绿潮种群暴发式增长的主要原因之一。

关键词(KeyWords)： 浒苔;孢子体;配子体;世代结构;生殖条件

Abstract:

Keywords:

基金项目(Foundation): 青岛市科技计划项目公共领域支撑类项目(09255hy);; 青岛市关键技术重大攻关计划项目(1281060432292;11-3-1-1-hy);; 国家科技基础性工作专项(2007FY210500);; 国家公益性行业科研专项(200805075)资助

作者(Author): 王浩东,姚雪,池姗,赵翠,金月梅,刘翠,朱明壮,刘涛

DOI: 10.16441/j.cnki.hdxb.2012.11.007

参考文献(References)：

• [1]Dan A,Hiraoka M,Ohno M,et al.Observations on the effect ofsalinity and photon fluence rate on the induction of sporulation andrhizoid formation in the green alga Enteromorpha prolifera(Müller)J.Agardh(Chlorophyta,Ulvales)[J].Fisheries Sci-ence,2002,68:1182-1188.
• [2]Choi C G,Takeuchi Y,Terawaki T,et al.Ecology of seaweedbeds on two types of artificial reef[J].Journal of Applied Phycol-ogy,2002,14:343-349.
• [3]王建伟,阎斌伦,林阿朋,等.浒苔(Enteromorpha prolifera)生长及孢子释放的生态因子研究[J].海洋通报,2007,26(2):60-65.
• [4]Sousa A I,Martins I,LillebA I,et al.Influence of salinity,nu-trients and light on the germination and growth of Enteromorphasp.spores[J].Journal of Experimental Marine Biology and Ecol-ogy,2007,341:142-150.
• [5]刘峰.黄海绿潮的成因以及绿潮浒苔的生理生态学和分子系统学研究[D].青岛:海洋研究所,2010:60-61.
• [6]邹定辉,陈雄文.高浓度CO2对条浒苔(Enteromorpha clathrata)生长和一些生理生化特征的影响[J].海洋通报,2002,21(5):39-45.
• [7]李瑞香,吴晓文,韦钦胜,等.不同营养盐条件下浒苔的生长[J].海洋科学进展,2009,2:211-216.
• [8]李俭平,赵卫红,付敏,等.氮磷营养盐对浒苔生长影响的初步探讨[J].海洋科学,2010,34(4):45-48.
• [9]Seibin A,Ikuko S.Variablity of morphological structure and modeof reproduction in Enteromorpha linza[J].Jap Journ Bot,1959,17(1):92-100.
• [10]Van Den Hoek C,Mann D G.Algae an introduction to phycology[M].Cambridge:Cambridge University Press,1996.
• [11]Bliding C.A critical survey of Europaen taxa in Ulvales.Part I.Capspsiphon,Percursaria,Blidingia,Enteromorpha[J].Op-era Botanica,1963,8:1-160.
• [12]Koeman R P T,Van Den Hoek C.The taxonomy of Enteromor-pha Link,1820,(Chlorophyceae)in the Natherlands.I.Thesection Enteromorpha[J].Algological studies,1982,32:279-330.
• [13]Hiraoka M,Dan A,Shimada S,et al.Different life histories ofEnteromorpha prolifera(Ulvales,Chlorophyta)from four riverson Shikoku Island,Japan[J].Phycologia,2003,42(3):275-284
• [14]叶乃好,张晓雯,毛玉泽,等.黄海绿潮浒苔(Enteromorpha pro-lifera)生活史的初步研究[J].中国水产科学,2008,15(5):853-857.
• [15]方宗熙,唐延林.用酶法分离紫菜营养细胞和原生质体获得成功[J].海洋通报,1982,5:94-96.
• [16]丁怀宇,马家海,王晓坤,等.缘管浒苔的单性生殖[J].2006,15(4):494-496
• [17]王晓坤,马家海,叶道才,等.浒苔(Enteromorpha prolifera)生活史的初步研究[J].海洋通报.2007,26(5):112-116
• [18]Kapraun D F,Bailey J C.Karyology and cytophotometric estima-tion of nuclear DNA variation in seven species of Ulvales(Chlo-rophyta)[J].Japanese Journal of Phycology,1992,40:13-24.
• [19]Y Le Gall,S Brown,Dominique Marie,et al.Quantification ofnuclear DNA and G-C content in marine macroalgae by flow cy-tometry of isolated nuclei[J].Protoplasma,1993,173:123-132.
• [20]Yayoi Kagami,Mariko Fujishita,Kazuyo Matsuyama-Serisawa,et al.DNA content of Ulva compressa(Ulvales,Chlorophyta)nuclei determined with laser scanning cytometry[J].Phycologi-cal Research,2005,53(1):77-83.
• [21]Serrao E A,Brawley S H,Hedman J,et al.Reproductive suc-cess of Fucus vesiculosus(Phaeophyceae)in the Baltic Sea[J].JPhycol,1999,35:254-269.

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