李梦,王敏,钟慎杰,邢泽宇,刘振辉

• 1:中国海洋大学海洋生命学院海洋生物多样性与进化研究所

摘要(Abstract)：

为了研究清道夫受体CD36在鱼类抗细菌免疫调控中的作用，本文将鳗弧菌(Vibrio anguillarum)腹腔注射到cd36基因敲除的斑马鱼(cd36~(-/-))和野生型斑马鱼(WT)中，发现突变体斑马鱼的活跃度和存活率均低于野生斑马鱼。分别取鳗弧菌(V.anguillarum)感染的cd36~(-/-)和WT斑马鱼头肾组织进行转录组学测序分析，发现二者差异表达的基因主要富集在补体系统(Complement system)、系统性红斑狼疮(Systemic lupus erythematosus)、细胞因子之间的相互作用(Interaction between cytokines)和病毒致癌机理(Viral carcinogenesis)等与免疫相关的信号途径中。运用实时荧光定量PCR技术进一步证实，与野生型斑马鱼相比，cd36~(-/-)斑马鱼中参与抗原呈递的mhc1和cd8基因、穿孔素基因、补体系统关键基因c3以及细胞因子基因il-4的表达都显著下调。综上，本研究证实CD36在斑马鱼抗鳗弧菌免疫调控中发挥重要作用。

关键词(KeyWords)： 斑马鱼;CD36蛋白;抗细菌免疫;MHC1蛋白;C3蛋白

Abstract:

Keywords:

基金项目(Foundation): 国家重点研究发展计划项目(2018YFD0900505);; 山东省自然科学基金项目(ZR2020MC050)资助~~

作者(Author): 李梦,王敏,钟慎杰,邢泽宇,刘振辉

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