孔思佳,赵阳国,姜明宏,王金鹏

• 1:中国海洋大学环境科学与工程学院
• 2:中国海洋大学海洋环境与生态教育部重点实验室

摘要(Abstract)：

为更好地去除海水循环养殖废水中的抗生素磺胺甲恶唑(SMX),以降低养殖废水排放造成的生态风险,从胶州湾近岸海水养殖池塘底泥中分离到一株SMX降解菌株O516,对其开展了SMX降解条件的优化,并考察了菌株附着生长特性及应用于废水处理系统的可行性。结果表明,通过16S rRNA基因序列分析,该菌被鉴定为库克动性球菌(Planococcuskocurii)O516;适宜培养温度为20～35℃,适宜pH为7.4;菌株在淡水中72 h可降解2.0 mg·L~(-1)的SMX,当盐度为35 g·L~(-1)时,72 h内可去除6.0 mg·L~(-1)的SMX。另外,菌株7 d内可在光滑的脱脂玻璃表面稳定生长出超过4个细胞厚度的生物膜,附着生长能力良好;将固定化菌株投加海水养殖废水处理反应器后,反应器对进水中1.0 mg·L~(-1)的SMX降解率自28.7%～40.0%提高至60.9%～68.1%。本研究表明,自海洋环境中分离的菌株库克动性球菌(P.kocurii)O516具有高效的SMX降解能力和耐盐特性,在海水养殖废水SMX治理中展现出很好的应用潜力。

关键词(KeyWords)： 海水养殖;磺胺甲恶唑;微生物降解;库克动性球菌(Planococcus kocurii);盐度

Abstract:

Keywords:

基金项目(Foundation): 中央高校基本科研业务费专项项目(201964004);; 国家自然科学基金面上项目(41977315)资助~~

作者(Author): 孔思佳,赵阳国,姜明宏,王金鹏

DOI: 10.16441/j.cnki.hdxb.20200041

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