谢坤秀,牛淑娜,迟会凯,张沛东

• 1:海水养殖教育部重点实验室(中国海洋大学)

摘要(Abstract)：

为探明不同种群鳗草(Zostera marina)种子的形态学特征及萌发特性，于2019年7月，采集山东半岛天鹅湖、俚岛湾和汇泉湾种群的鳗草种子，开展了不同种群种子形态学特征、营养贮存物质、萌发特性与实生苗存活、生长和光合生理的对比分析。研究显示：(1)汇泉湾种群的鳗草种子规格最大，平均长径达3.69 mm,平均百粒质量达0.98 g;(2)各种群鳗草种子的营养贮存物质均以总糖为主(>46%),其中天鹅湖种群种子的总糖含量最高，达62%,显著高于俚岛湾和汇泉湾种群种子的总糖含量(p<0.05);(3)经人工促萌，天鹅湖种群的种子萌发率和实生苗存活率显著高于汇泉湾种群，分别是其1.6和3.0倍(p<0.05);(4)不同种群种子形态学特征和营养贮存物质的聚类分析显示，不同种群间种子存在明显差异，其中天鹅湖和俚岛湾种群的种子聚为一类，而汇泉湾种群的种子单独聚为一类；(5)主成分分析(PCA)和曲线拟合显示，种子萌发率及实生苗存活率与种子总糖含量呈显著的线性正相关关系，与种子质量呈显著的线性负相关关系(p<0.05);(6)天鹅湖种群的实生苗规格、光合色素含量和光合速率均为最高值，显著高于俚岛湾种群(p<0.05)。综合分析认为，山东半岛不同种群的鳗草种子在形态学特征和萌发特性上存在显著差异，其中种子的萌发特性主要取决于种子的总糖含量。

关键词(KeyWords)： 鳗草;种子;规格;萌发率;总糖含量;实生苗

Abstract:

Keywords:

基金项目(Foundation): 国家重点研究发展计划项目(2019YFD0901302);; 国家自然科学基金项目(42076100);; 中央高校基本科研业务费专项(201822021)资助~~

作者(Author): 谢坤秀,牛淑娜,迟会凯,张沛东

DOI: 10.16441/j.cnki.hdxb.20210023

参考文献(References)：

• [1] Short F T,Wyllie-Echeverria S.Natural and human-induced disturbance of seagrasses[J].Environmental Conservation,1996,23(1):17-27.
• [2] 李文涛，张秀梅.海草场的生态功能[J].中国海洋大学学报(自然科学版),2009,39(5):933-939.Li W T,Zhang X M.The ecological functions of seagrass meadows[J].Periodical of Ocean University of China,2009,39(5):933-939.
• [3] 刘旭佳，周毅，杨红生，等.大叶藻碎屑作为刺参食物来源的实验研究[J].海洋科学，2013,37(10):32-38.Liu X J,Zhou Y,Yang H S,et al.Evaluation of eelgrass debris as a food source for Apostichopus japonicas[J].Marine Sciences,2013,37(10):32-38.
• [4] 邱广龙，周浩郎，覃秋荣，等.海草生态系统与濒危海洋哺乳动物儒艮的相互关系[J].海洋环境科学，2013,32(6):970-974.Qiu G L,Zhou H L,Qin Q R,et al.Interactions between seagrass ecosystem and the endangered marine mammal dugong[J].Marine Environmental Science,2013,32(6):970-974.
• [5] Gambi M C,Nowell A,Jumars P A.Flume observations on flow dynamics in Zostera marina (eelgrass) beds[J].Marine Ecology Progress Series,1990,61(1-2):159-169.
• [6] Bos A R,Bouma T J,de Kort G L J,et al.Ecosystem engineering by annual intertidal seagrass beds:Sediment accretion and modification[J].Estuarine Coastal and Shelf Science,2007,74(1-2):344-348.
• [7] Duarte C M,Chiscano C L.Seagrass biomass and production:A reassessment[J].Aquatic Botany,1999,65(1-4):159-174.
• [8] Waycott M,Duarte C M,Carruthers T J B,et al.Accelerating loss of seagrasses across the globe threatens coastal ecosystems[J].Proceedings of the National Academy of Sciences,2009,106(30):12377-12381.
• [9] Lent F V,Verschnure J M.Comparative study on populations of Zostera marina L.(eelgrass):Experimental germination and growth[J].Journal of Experimental Marine Biology & Ecology,1995,185(1):77-91.
• [10] Koch E W,Dawes C J.Influence of salinity and temperature on the germination of Ruppia maritima L.from the North Atlantic and Gulf of Mexico[J].Aquatic Botany,1991,40(4):387-391.
• [11] Maun M A.Seed germination and seedling establishment of Calamovilfa longifolia on Lake Huron sand dunes[J].Canadian Journal of Botany,1981,59(59):460-469.
• [12] Westoby M,Kidson R.Seed mass and seedling dimensions in relation to seedling establishment[J].Oecologia,2000,125(1):11-17.
• [13] Roach D A,Wulff R D.Maternal effects in plants[J].Annual Review of Ecology & Systematics,1987,18:209-235.
• [14] 柯文山，钟章成，席红安，等.四川大头茶地理种群种子大小变异及对萌发、幼苗特征的影响[J].生态学报，2000,20(4):697-701.Ke W S,Zhong Z C,Xi H A,et al.The variation of seed sizes of Gordonia acuminata geographic populations and its effect on seed germination and seedling[J].Acta Ecologica Sinica,2000,20(4):697-701.
• [15] 王桔红，崔现亮，陈学林，等.中、旱生植物萌发特性及其与种子大小关系的比较[J].植物生态学报，2007,31(6):1037-1045.Wang J H,Cui X L,Chen X L,et al.Comparative study of seed germination,seed size and their relationships in mesad and siccocolous[J].Journal of Plant Ecology,2007,31(6):1037-1045.
• [16] Wahid N,Bounoua L.The relationship between seed weight,germination and biochemical reserves of maritime pine (Pinus pinaster Ait.) in Morocco[J].New Forests,2013,44(3):385-397.
• [17] 徐少春.山东沿海典型海草床鳗草种子生态学研究[D].青岛：中国科学院海洋研究所，2017.Xu S C.A Study on Seed Ecology of the Eelgrass Zostera marina L.in Typical Seagrass Meadows in Shandong[D].Qingdao:Institute of Oceanology,Chinese Academy of Sciences,2017.
• [18] 洑香香，刘红娜，周晓东，等.山茱萸种子形态变异及与环境因子的相关性[J].生态学杂志，2013,32(1):27-32.Fu X X,Liu H N,Zhou X D,et al.Morphological variation of Cornus officinalis seeds in relation to environmental factors[J].Chinese Journal of Ecology,2013,32(1):27-32.
• [19] 杨敏，王相琴.种子生产与管理[M].合肥：合肥工业大学出版社，2013.Yang M,Wang X Q.Seed Production and Management[M].Hefei:Hefei University of Technology Press,2013.
• [20] 陈智慧，史梅，王秋香，等.用凯氏定氮法测定食品中的蛋白质含量[J].新疆畜牧业，2008(5):22-24.Chen Z H,Shi M,Wang Q X,et al.The protein content in food determined by Kjeldahl method[J].Animal Husbandry in Xinjiang,2008(5):22-24.
• [21] 国家标准化管理委员会.食物中脂肪的测定：GB/T 5009.6—2003[S].北京：中国标准化出版社，2003.Standardization Administration.Determination of Fat in Food:GB/T 5009.6—2003[S].Beijing:China Standardization Press,2003.
• [22] 赵凯，许鹏举，谷广烨.3,5-二硝基水杨酸比色法测定还原糖含量的研究[J].食品科学，2008,29(8):534-536.Zhao K,Xu P J,Gu G Y.Study on determination of reducing sugar content using 3,5-dinitrosalicylic acid method[J].Food Science,2008,29(8):534-536.
• [23] Liu J,Zhang P D,Guo D,et al.Annual change in photosynthetic pigment contents of Zostera marina L.in Swan Lake[J].African Journal of Biotechnology,2011,10(79):18194-18199.
• [24] 岳华峰，邵文豪，井振华，等.苦槠种子形态性状的地理变异分析[J].林业科学研究，2010,23(3):453-456.Yue H F,Shao W H,Jing Z H,et al.Geographic variation of seed characters of Castanopsis sclerophylla[J].Forest Research,2010,23(3):453-456.
• [25] Xu S C,Wang P M,Zhou Y,et al.New insights into different reproductive effort and sexual recruitment contribution between two geographic Zostera marina L.populations in temperate China[J].Frontiers in Plant Science,2018,9:15.
• [26] Combs A R,Jarvis J C,Kenworthy W J.Quantifying variation in Zostera marina seed size and composition at the species′ southern limit in the Western Atlantic:Implications for eelgrass population resilience[J].Estuaries and Coasts,2021,44:367-382.
• [27] J?rgensen M S,Labouriau R,Olesen B,et al.Seed size and burial depth influence Zostera marina L.(eelgrass) seed survival,seedling emergence and initial seedling biomass development[J].PLoS One,2019,14(4):e0215157.
• [28] Felger R,Moser M B.Eelgrass (Zostera marina L.) in the gulf of California:Discovery of its nutritional value by the Seri Indians[J].Science,1973,181(4097):355-356.
• [29] Harper J L,Lovell P H,Moore K G.The shapes and sizes of seeds[J].Annual Review of Ecology and Systematics,1970,1(1):327-356.
• [30] 张世挺，杜国祯，陈家宽.种子大小变异的进化生态学研究现状与展望[J].生态学报，2003,23(2):353-364.Zhang S T,Du G Z,Chen J K.The present situation and prospect of studies on evolutionary ecology of seed size variation[J].Acta Ecologica Sinica,2003,23(2):353-364.
• [31] Smith W E,Fitzsimmons J E.Maternal inheritance of seed weight in Flax[J].Canadian Journal of Genetics and Cytology,1965,7(4):658-662.
• [32] 李渊，孙典荣，李文涛，等.基于matK、rbcL和ITS序列的5种大叶藻系统发育研究[J].水产学报，2011,35(2):183-191.Li Y,Sun D R,Li W T,et al.Phylogenetic relationship in five Zosteraceae species based on matK,rbcL and ITS partial sequences[J].Journal of Fisheries of China,2011,35(2):183-191.
• [33] 栗宏林，张志翔，张鑫.小桐子不同产地种子性状及苗期生长差异研究[J].干旱区资源与环境，2010,24(2):204-208.Li H L,Zhang Z X,Zhang X.Study on seed morphology and seedling growth of Jatropha curcas L.from different provenances[J].Journal of Arid Land Resources and Environment,2010,24(2):204-208.
• [34] Wyllie-Echeverria S,Cox fls P A,Churchill A C,et al.Seed size variation within Zostera marina L.(Zosteraceae)[J].Botanical Journal of the Linnean Society,2003,142(3):281-288.
• [35] 彭鸿嘉.六种牧草种子大小和播种深度对出苗的影响[J].草业科学，2001,18(6):30-35.Peng H J.Effects of seed size and seedling depth on emergence of six perennial grasses[J].Pratacultural Science,2001,18(6):30-35.
• [36] 于洪军，徐兴永，李萍，等.青岛市浮山湾、汇泉湾、崂山湾海滩与海底沉积环境分析[J].海岸工程，2003,22(3):12-18.Yu H J,Xu X Y,Li P,et al.Analysis of beach and submarine sediment environments in the Fushan Bay,Huiquan Bay and Laoshan Bay of Qingdao[J].Coastal Engineering,2003,22(3):12-18.
• [37] 郭栋，张沛东，张秀梅，等.荣成俚岛近岸海域大叶藻的生态学研究[J].中国海洋大学学报(自然科学版),2010,40(9):51-55.Guo D,Zhang P D,Zhang X M,et al.Ecological study on eelgrass of inshore areas in Lidao town of Rongcheng city[J].Periodical of Ocean University of China,2010,40(9):51-55.
• [38] Orth R J,Luckenbach M,Moore K A.Seed dispersal in a marine macrophyte:Implications for colonization and restoration[J].Ecology,1994,75(7):1927-1939.
• [39] Tanner C E,Parham T,Shafer D.Growing Zostera marina (eelgrass) from seeds in land-based culture systems for use in restoration projects[J].Restoration Ecology,2010,18(4):527-537.
• [40] Cabaco S,Santos R.Reproduction of the eelgrass Zostera marina at the species southern distributional limit in the Eastern Atlantic[J].Marine Ecology,2010,31(2):300-308.
• [41] Milberg P,Lamont B B.Seed/cotyledon size and nutrient content play a major role in early performance of species on nutrient-poor soils[J].New Phytologist,1997,137(4):665-672.
• [42] Jarvis J,Moore K.Effects of seed source,sediment type,and burial depth on mixed-annual and perennial Zostera marina L.seed germination and seedling establishment[J].Estuaries Coasts 2015,38(3):964-978.
• [43] Uchida M,Miyoshi T,Kaneniwa M,et al.Production of 16.5% v/v ethanol from seagrass seeds[J].Journal of Bioscience and Bioengineering,2014,118(6):646-650.
• [44] Irving D W,Breda V A,Becker R,et al.Anatomy and composition of Zostera marina L.:A potential new crop[J].Ecology of Food and Nutrition,1988,20(4):263-274.
• [45] Belzunce M,Navarro R M,Rapoport H F.Seed and early plantlet structure of the Mediterranean seagrass Posidonia oceanica[J].Aquatic Botany,2005,82(4):269-283.
• [46] 黄升谋，张宏波.小麦种子萌发过程中糖类的变化[J].湖北文理学院学报，2016,37(11):31-34.Huang S M,Zhang H B.Changes of sugar during the germination of wheat seeds[J].Journal of Hubei University of Arts and Science,2016,37(11):31-34.
• [47] 蒋敏明.杂交水稻高活力种子适宜收获期的研究[D].长沙：湖南农业大学，2015.Jiang M M.Study of Suitable Harvest Dates for Higher Vigor Seed Production of Hybrid Rice[D].Changsha:Hunan Agricultural University,2015.
• [48] Kuo J,Hartog C D,Larkum A W,et al.Seagrass Morphology,Anatomy,and Ultrastructure[M].Dordrecht:Springer SBS,2006.
• [49] Taylor A R A.Studies of the development of Zostera marina L.II.Germination and seedling development[J].Can J Bot,1957,35:681-695.
• [50] Venable D L,Brown J S.The selective interactions of dispersal,dormancy,and seed size as adaptations for reducing risk in variable environments[J].American Naturalist,1988,131(3):360-384.
• [51] 陈建敏，孙德兰.莲种子萌发和幼苗生长时期营养物质的代谢变化[J].植物学报，2005,22(5):541-548.Chen J M,Sun D L.Effects of glucose stress on seeds germination and seedlings growth of Arabidopsis thaliana[J].Chinese Bulletin of Botany,2005,22(5):541-548.
• [52] Sugiura H,Hiroe Y,Suzuki T,et al.The carbohydrate catabolism of Zostera marina influenced by lower salinity during the pre-germination stage[J].Fisheries Science,2009,75(5):1205-1217.
• [53] 李荣平，蒋德明，刘志民，等.沙埋对六种沙生植物种子萌发和幼苗出土的影响[J].应用生态学报，2004,15(10):1865-1868.Li R P,Jiang D M,Liu Z M,et al.Effects of sand-burying on seed germination and seedling emergence of six psammophytes species[J].Chinese Journal of Applied Ecology,2004,15(10):1865-1868.
• [54] 宗文杰，刘坤，卜海燕，等.高寒草甸51种菊科植物种子大小变异及其对种子萌发的影响研究[J].兰州大学学报，2006,42(5):52-55.Zong W J,Liu K,Bu H Y,et al.The mode of seed size variation and the effects of seed size on fifty-one species of composite plants in a alpine meadow[J].Journal of Lanzhou University,2006,42(5):52-55.
• [55] 刘振恒，徐秀丽，卜海燕，等.青藏高原东部常见禾本科植物种子大小变异及其与萌发的关系[J].草业科学，2006,23(11):53-57.Liu Z H,Xu X L,Bu H Y,et al.Seed size variation and its relationship to germination for familiar species of Gramineae on the eastern Qinghai-Tibet Plateau[J].Pratacultural Science,2006,23(11):53-57.

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