史宝,柳学周,刘永山,张言祥,高全义,徐永江,王滨,姜燕,宋雪松

• 1:青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室中国水产科学研究院黄海水产研究所
• 2:上海海洋大学水产与生命学院
• 3:大连富谷水产有限公司

摘要(Abstract)：

为探讨黄条鰤(Seriola aureovittata)对盐度渐变的应激响应机制,设置盐度5、10、15、20、29和35共6个盐度渐变点,对幼鱼的消化酶活力、超氧化物歧化酶(SOD)和甲状腺激素(T4)等生理指标进行了检测和分析。研究显示:胃、肠、肝脏和幽门盲囊的脂肪酶的活性均在盐度29渐变点最高,在低盐度渐变点这4个组织的脂肪酶活性均随盐度降低而降低。肠和肝脏的蛋白酶活性在盐度29渐变点达到峰值,幽门盲囊的蛋白酶活性则在盐度35渐变点最高,且与其他盐度渐变点间有显著性差异(P<0.05)。胃、肠、肝脏的淀粉酶活性在盐度29渐变点最高,而幽门盲囊的淀粉酶活性在盐度35渐变点最高,但与盐度29渐变点无显著性差异(P>0.05)。血清SOD活性在盐度5渐变点显著低于其他盐度渐变点(P<0.05),SOD活性在盐度20和35渐变点与29渐变点无显著性差异(P>0.05)。血清T4浓度随着盐度的升高或降低均升高,盐度20渐变点与29渐变点的T4浓度无显著性差异(P>0.05)。研究显示,自然海水盐度29是黄条鰤幼鱼存活的适宜盐度,且黄条鰤幼鱼能较快适应略低盐度(20～29),但较低或较高盐度渐变点的消化酶活力和抗应激指标则显著异常。盐度渐变可引起黄条鰤幼鱼的消化生理、抗氧化水平和T4浓度的变化,黄条鰤幼鱼对外界盐度变化表现出较强的适应性。

关键词(KeyWords)： 黄条鰤;盐度渐变;消化酶;超氧化物歧化酶;甲状腺激素;应激响应

Abstract:

Keywords:

基金项目(Foundation): 中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金项目(2018GH17);中国水产科学研究院黄海水产研究所基本科研业务费项目(20603022017016);; 青岛海洋科学与技术国家实验室海洋渔业科学与食物产出过程功能实验室开放课题项目(2017-3A01);; 青岛市民生科技计划项目(17-3-3-61-nsh);; 国家自然科学基金项目(31772829);; 国家海水鱼技术体系项目(CARS-47)资助~~

作者(Author): 史宝,柳学周,刘永山,张言祥,高全义,徐永江,王滨,姜燕,宋雪松

DOI: 10.16441/j.cnki.hdxb.20180187

参考文献(References)：

• [1] Peter L,James S B.Metabolic responses to salinity acclimation in juvenile shortnose sturgeon Acipenser brevirostrum[J].Aquaculture,2003,219(1-4):891-909.
• [2] 郭勤单,王有基,吕为群.温度和盐度对褐牙鲆幼鱼渗透生理及抗氧化水平的影响[J].水生生物学报,2014,38(1):58-67.Guo Q D,Wang Y J,Lü W Q.Combined effects of temperature and salinity on the physiological osmotic induction and antioxidant responses in the juvenile Japanese flounder (Paralichthys olivaceus)[J].Acta Hydrobiologica Sinica,2014,38(1):58-67.
• [3] 罗鸣钟,关瑞章,靳恒.盐度对花鳗鲡和太平洋双色鳗鲡幼鳗生长性能及消化酶活力的影响[J].水生生物学报,2015.39(4):653-660.Luo M Z,Guan R Z,Jin H.Effects of the salinity on the growth performance and digestive enzyme activities of Anguilla marmorata elver and A.bicolor pacifica elver[J].Acta Hydrobiologica Sinica,2015,39(4):653-660.
• [4] 邓平平,施永海,汪洋,等.盐度对长江刀鲚幼鱼非特异性免疫酶和消化酶活力的影响[J].大连海洋大学学报,2016,31(5):533-537.Deng P P,Shi Y H,Wang Y,et al.Effects of salinity on activities of non-specific immune and digestive enzymes in juvenile estuarine tapertail anchovy Coilia nasus [J].Journal of DaLian Ocean University,2016,31(5):533-537.
• [5] Agrawal V P,Sastry K V,Kaushab S K.Digestive enzymes of three teleost fishes[J].Acta Physiologica Acdemiae Scientiarum Hungaricae,1975(46):93-98.
• [6] Das K M,Tripathi S D.Studies on the digestive enzymes of Ctenopharyngodon idella (Val)[J].Aquaculture,1991(165):311-312.
• [7] Sinha A K,Abdelgawad H,Zinta G,et al.Nutritional status as the key modulator of antioxidant responses induced by high environmental ammonia and salinity stress in European Sea Bass(Dicentrarchus labrax)[J].PLoS ONE,2015,10(8):e0135091.
• [8] Sui Y M,Huang X Z,Kong H,et al.Physiological responses to salinity increase in blood parrotfish(Cichlasoma synspilum♀×Cichlasoma citrinellum♂)[J].SpringerPlus,2016,5:1246.
• [9] Ruiz-Jarabo I,Klaren P H M,Louro B,et al.Characterization of the peripheral thyroid system of Gilthead seabream acclimated to different ambient salinities[J].Comparative Biochemistry and Physiology,2017,203:24-31.
• [10] Chai X L,Li X X,Lu R M,et al.Karyotype analysis of the yellowtail kingfish Seriola lalandi lalandi(Perciformes:Carangidae) from South Australia[J].Aquculture Research,2009,40(15):1735-1741.
• [11] 柳学周,徐永江,李荣,等.黄条鰤(Seriola aureovittata)肌肉营养组成分析与评价[J].渔业科学进展,2017,38(1):128-135.Liu X Z,Xu Y J,Li R,et al.Analysis and evaluation of nutritional composition of the muscle of yellowtail kingfish(Seriola aureovittata)[J].Progress in Fishery Sciences,2017,38(1):128-135.
• [12] 史宝,刘永山,柳学周,等.黄条鰤(Seriola aureovittata)染色体核型分析[J].渔业科学进展,2017,38(1):136-141.Shi B,Liu Y S,Liu X Z,et al.Study on the karyotype of yellowtail kingfish (Seriola aureovittata)[J].Progress in Fishery Sciences,2017,38(1):136-141.
• [13] 李荣,徐永江,柳学周,等.黄条鰤(Seriola aureovittata)形态度量与内部结构特征[J].渔业科学进展,2017,38(1):142-149.Li R,Xu Y J,Liu X Z,et al.Morphometric analysis and internal anatomy of yellowtail kingfish(Seriola aureovittata)[J].Progress in Fishery Sciences,2017,38(1):142-149.
• [14] Bronwyn M G,Douglas J F,Neil L A.Aging methods for yellowtail kingfish,Seriola lalandi,and results from age- and size-based growth models[J].Fishery Bulletin,1999,97:812-827.
• [15] 沈永龙,戈贤平,黄金田,等.盐度对瘤背石磺(Onchidium struma)消化酶活性的影响[J].动物营养学报,2012,24(9):1839-1846.Sheng Y L,Ge X P,Huang J T,et al.The effect of salinity on digestive enzyme activity of Onchidium struma [J].Chinese Journal of Animal Nutrition.2012,24(9):1839-1846.
• [16] 许星鸿,阁斌伦,徐加涛,等.盐度对日本蟳免疫生理指标及消化酶活力的影响[J].水产科学,2013,32(7):373-377.Xu X H,Ge B L,Xu J T,et al.Effects of salinity on immune physiological indexes and digestive enzyme activity of Scylla serrata[J].Fisheries Science,2013,32(7):373-377.
• [17] 成永洲.海水盐度对斜带石斑鱼幼鱼生长及生理生化的影响[D].南京:南京农业大学,2015:1-71.Cheng Y Z.The Effect of Salinity on Growth and Physiolgy Biochemical of Juvenile Epinephelus coiodies Reared in the Seawater[D].Nanjing:Nanjing Agricultural University,2015:1-71.
• [18] 庄平,章龙珍,田宏杰,等.盐度对史氏鲟幼鱼消化酶活力的影响[J].中国水产科学,2008,15(2):198-203.Zhuang P,Zhang L Z,Tian H J,et al.The effect of salinity on digestive enzyme activity of Acipenser schrenckii [J].Journal of Fishery Sciences of China,2008,15(2):198-203.
• [19] 罗集光,章龙珍,庄平,等.盐度对点篮子鱼消化酶活性的影响[J].海洋渔业,2011,33(1):33-38.Luo J G,Zhang L Z,Zhuang P,et al.Effect of salinity on digestive enzyme zctivity in Siganus guttatas[J].Marine Fisheries,2011,33(1):33-38.
• [20] 强俊,王辉,李瑞伟,等.盐度对奥尼罗非鱼仔、稚鱼生长、存活及其消化酶活力的影响[J].南方水产,2009,10(5):8-14.Qiang J,Wang H,Li R W,et al.The effects of salinity on the growth,survival and digestive enzyme activity of Larval hybrid tilapia (Orechromis nifocoticus×O.aureus) [J].South China Fisherise Science,2009,10(5):8-14.
• [21] 田相利,任晓伟,董双林,等.温度和盐度对半滑舌鳎幼鱼消化酶活性的影响[J].中国海洋大学学报(自然科学版),2008,38(6):895-901.Tian X L,Ren X W,Dong S L,et al.Studies on the Specific activities of digestive enzymes of Cynoglossus semilaevis Günther at different salinities and temperatures[J].Periodical of Ocean University of China,2008,38(6):895-901.
• [22] 辛碧芬,谢进金,左巧阳,等.几种金属离子对鲢肝胰脏淀粉酶的影响[J].水生态学杂志,2009,2(3):91-95.Xi B F,Xie J T,Zuo Q Y,et al.The effects of several metal ions on the liver pancreatic amylase of Silver carp[J].Journal of water ecology,2009,2(3):91-95.
• [23] 史宝,柳学周,刘永山,等.盐度渐变过程对黄条鰤(Seriola aureovittata)幼鱼渗透调节的影响[J].海岸工程,2019,38(1):63-70.Shi B,Liu X Z,Liu Y S,et al.Effects of the gradual salinity change on the osmotic regulation of juvenile yellowtail kingfish (Seriola aureovittata)[J].Coastal Engineering,2019,38(1):63-70.
• [24] 康自强.盐度胁迫对星洲红鱼生长、消化酶和免疫因子活性及肌肉品质的影响[D].厦门:集美大学,2014:1-53.Kang Z Q.Study on the Influences of Salinity on Growth,Digestive Enzyme and Immue Factor Activities and Muscle Quality of Red tilapia[D].Xiamen:Jimei University,2014:1-53.
• [25] Fang L S,ChIou S F.Effect of salinity on the activities of digestive protease from the Tilapia fish,Oreochromis ruloticus,in different culture environments[J].Comparative Biochemistry & Physiology,1989,93(2):439-443.
• [26] 王妤,庄平,章龙珍,等.盐度对点篮子鱼的存活、生长及抗氧化防御系统的影响[J].水产学报,2011,135(1):66-73.Wang Y,Zhuang P,Zhang L Z,et al.Effects of salinity on survival,growth and antioxidant defense system of Siganus guttatus [J].Journal of Fisheries of China,2011,135(1):66-73.
• [27] Martínez R M,Morales A E,Sanz A.Antioxidant defenses in fish:Biotic and abiotic factors[J].Reviews in Fish Biology and Fisheries,2005,15(1-2):75-88.
• [28] 尹飞,孙鹏,彭士明,等.低盐度胁迫对银鲳幼鱼肝脏抗氧化酶、鳃和肾脏ATP酶活力的影响[J].应用生态学报,2011,22(4):1059-1066.Yin F,Sun P,Peng S M,et al.Effects of low salinity stress on the antioxidant enzyme activities in juvenile Pampus argenteus liver and the ATPase activities in its gill and kidney[J].Chinese Journal of Applied Ecology,2011,22(4):1059-1066.
• [29] An K W,Kim N N,Shin H S,et al.Profiles of antioxidant gene expression and physiological changes by thermal and hypoosmotic stresses in black porgy (Acanthopagrus schlegeli)[J].Comparative Biochemistry and Physiology,2010,156(2):262-268.
• [30] MHR J R,Sved D W,Felton S P.Temporal changes in feral AHH and SOD activity in feral spot from the Elizabeth river a polluted sub-estuary[J].Marine Environmental Research,1987,23:89-101.
• [31] Peyghan R,Enayati A,Sabzevarizadeh M.Effect of salinity level on TSH and thyroid hormones of grass carp,Ctenophayngodon idella[J].Veterinary Research Forum,2013,4 (3):175-178.
• [32] 王义强,黄世蕉,赵维信,等.鱼类生理学[M].上海:上海科学技术出版社,1990,188-191.Wang Y Q,Huang S J,Zhao W X,et al.Fish Physiology[M].Shanghai:Shanghai Scientific & Technical Publishers,1990:188-191.

文章评论(Comment)：