沈洪波,韩峰,林育姿,王传宁,于文功

• 1:中国海洋大学教育部海洋药物重点实验室 海洋药物与食品研究所
• 2:中国海洋大学教育部海洋药物重点实验室
• 3:海洋药物与食品研究所
• 4:中国海洋大学教育部海洋药物重点实验室

摘要(Abstract)：

Pseudomonassp.QDA是从海水中发现的产生褐藻多糖的细菌,无致病性。为提高QDA菌株褐藻多糖的产量,本文利用PCR克隆了粘性菌株P.aeruginosaFRD1的algT基因,连接入质粒pMF36-Kan,构建了重组表达载体pMF36-Kan-algT。利用三亲接合法将pMF36-Kan-algT转入菌株QDA中。糖醛酸含量测定结果表明,algT基因过量表达菌株的褐藻多糖产量比出发菌株QDA平均提高了3.3倍,其中菌株A25提高了4.5倍。通过培养条件的进一步优化,A25菌株的褐藻多糖产量与菌株QDA相比提高到8.18倍。

关键词(KeyWords)： 假单胞菌;褐藻多糖;algT基因;过量表达

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(30371683)资助

作者(Author): 沈洪波,韩峰,林育姿,王传宁,于文功

DOI: 10.16441/j.cnki.hdxb.2006.04.024

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