门荣新,杨官品,沈锡权,廖梅杰,管晓菁

• 1:中国海洋大学海洋生命学院
• 2:中国海洋大学海洋生命学院
• 3:中国海洋大学海洋生命学院
• 4:中国海洋大学海洋生命学院

摘要(Abstract)：

设计了桡足类特异引物,从胶州湾浮游生物混合DNA中选择性扩增了浮游桡足类18S核糖体RNA基因片段,建立了该基因片段变异类型文库。从文库中随机选择83个克隆,对所含的18S核糖体RNA基因片段进行了V .spI和BshNIRFLP分析,发现5种操作分类单元(OTU) ,并对这些OTU的代表克隆进行了测序。分析发现获得的克隆均属于桡足亚纲。根据各OTU覆盖的克隆数计算的胶州湾浮游桡足类遗传多样性指数为0 .96。特定基因序列分析能提供易整合、易比较的多样性数据,也是建立浮游生物群落结构分子生物学剖分方法的基础和形态分类的补充。

关键词(KeyWords)： 桡足亚纲;生物多样性;18S核糖体RNA基因

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金 (40 1 760 2 8)资助

作者(Author): 门荣新,杨官品,沈锡权,廖梅杰,管晓菁

DOI: 10.16441/j.cnki.hdxb.2005.03.008

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