基于耳石微化学特征的大麻哈鱼生境履历分析及其在群组鉴别中的应用Analysis of Life History and Population Identification of Chum Salmon (Oncorhynchus keta) Based on Otolith Microchemical Characteristics
王继隆,刘伟,杨文波,李培伦,唐富江,鲁万桥,杨健,姜涛
摘要(Abstract):
为研究大麻哈鱼(Oncorhynchus keta)生活史特征及不同群组间的鉴别,本文采用电子微探针技术分析自然野生、人工增殖锶标记、人工增殖物理标记和人工养殖4组大麻哈鱼耳石样本的Sr浓度及Sr/Ca值,反演其生境履历。定量线分析结果表明,耳石Sr/Ca值波动显著,根据Sr/Ca值的特征划分了各群组大麻哈鱼淡、咸水间的生活史阶段。根据大麻哈鱼生活史特征及耳石径长参数差异的分析结果将各群组间个体进行了有效辨别。其中锶标记组个体耳石标记区Sr/Ca值达到17.033×10~3,显著高于其他组,证实了锶标记方法的有效性。对大麻哈鱼人工增养殖组和自然野生组早期淡水生活阶段耳石径长参数(R_(f1)、R_L)以及淡水系数(R_C)进行对比分析,结果显示:自然野生组中120μm≤R_(f1 )≤200μm, 20μm≤R_L≤30μm, 0.06≤R_C≤0.10;人工增养殖组中240μm≤R_(f1 )≤470μm, 80μm≤R_L≤200μm, 0.15≤R_C≤0.29。两群组间的参数差异显著,可作为群组间个体辨别的依据。本研究结果表明,耳石微化学分析技术能够有效分析大麻哈鱼的关键生活史特征,且可据此开展大麻哈鱼群组间的鉴别。研究结果可为大麻哈鱼生活史研究及增殖放流效果评估提供有效的方法。
关键词(KeyWords): 大麻哈鱼;耳石微化学;电子探针;生活史;群组鉴别
基金项目(Foundation): 黑龙江省自然科学基金项目(YQ2019C034);; 农业农村部财政专项物种资源保护类项目(2130135)资助~~
作者(Author): 王继隆,刘伟,杨文波,李培伦,唐富江,鲁万桥,杨健,姜涛
DOI: 10.16441/j.cnki.hdxb.20200241
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