橙色莫桑比克罗非鱼和荷那龙罗非鱼的AFLP分析AFLP Analysis of Two Species of Tilapia,O.mossambicus and O.hornorum
杨淞,叶星,卢迈新,黄樟翰,白俊杰
摘要(Abstract)：
运用AFLP分子标记技术检测了橙色莫桑比克罗非鱼(Oreochromis mossambicus)和荷那龙罗非鱼(Oreochromishornorum)的种群遗传多样性。提取2种鱼的基因组DNA,经EcoRⅠ和MseⅠ限制性内切酶酶切并加接头,从16对选扩引物组合中选出3对扩增结果较好的引物进行PCR扩增。扩增产物经PAGE胶电泳、银染,检测其多态性。3对引物在2种罗非鱼的40个个体中共扩增出94条带,其中多态性条带76条,占总扩增条带的80.9%,2个群体的多态性条带比例分别为71.6%和52.4%。根据Nei氏(1978)统计分析得出橙色莫桑比克罗非鱼种群内遗传相似系数为0.765,荷那龙罗非鱼的为0.821,表明2个罗非鱼种群均具有较高的遗传多样性,橙色莫桑比克罗非鱼种群内遗传多样性相对更高些。2种罗非鱼的种间遗传距离为0.279,推测该杂交组合可能具有杂种优势。
关键词(KeyWords)： 橙色莫桑比克罗非鱼;荷那龙罗非鱼;AFLP;遗传多样性
基金项目(Foundation): 广东省农业重大专项(2003A2010501);; 广东省农业攻关项目(2004A20105001);; 农业部“948”项目(2001-479-3);; 中国水产科学研究院重点项目(2001-1-5);; 广东省重大科技兴渔项目(A200501B03);; 广东省农业攻关项目(2006B20201057)资助
作者(Author): 杨淞,叶星,卢迈新,黄樟翰,白俊杰
DOI: 10.16441/j.cnki.hdxb.2006.06.018
参考文献(References)：
- [1]Vos P,Hogers R,Bleeker M,et al.AFLP:a new technique forDNA fingerprinting[J].Nucleic Acids Research,1995,23(21):4407-4414.
- [2]李珊,赵桂仿.AFLP分子标记及其应用[J].西北植物学报,2003,23(5):830-836.
- [3]Junbin Zhanga,Liangmin Huanga,Heqiang Huo.Larval identifica-tion of lutjanus bloch in nansha coral reefs by AFLP molecular method[J].Journal of Experimental Marine Biology and Ecology,2004(298):3-20.
- [4]Wang Zhiyong,Kwok H T,Ka Hou Chu.Applications of AFLPtechnology in genetic and phylogenetic analysis of penaeid shrimp[J].Biochemical Systematics and Ecology,2004(32):399-407.
- [5]Li Li,Guo Ximing.AFLP-Based genetic linkage maps of the pacificoysterCrassotrea gigasthunberg[J].Marine Biotechnology,2004(6):26-36.
- [6]Wang Z,Jayasankar P,Khoo S K,et al.AFLP fingerprinting revealsgenetic variability in common carp stocks from Indonesia[J].AsianFisheries Science,2000(13):139-147.
- [7]王志勇,王艺磊,林利民.利用AFLP指纹技术研究中国沿海真鲷群体的遗传变异和趋异[J].水产学报,2001,25(4):289-293.
- [8]潘洁,包振民,赵洋.栉孔扇贝不同地理种群的遗传多态性分析[J].高技术通讯,2002,12:78-82.
- [9]Kocher T D,Lee W J,Sobol Wska H,et al.A genetic map of cichli-dae fish,the tilapia(oreochromis niloticus)[J].Genetics,1998,148:1225-1232.
- [10]Jeremy J A,Shingo S,Avner C.Breeding new strains of tilapia:de-velopment of an artificial center of origin and linkage map based onAFLP and microsatellite loci[J].Aquaculture,2000,185:43-56.
- [11]Avner C,Eric M Hallerman,Micha Ron,et al.Detection of a chro-mosomal region with two quantitative trait loci,affecting cold toler-ance and fish size,in an F2 tilapia hybrid[J].Aquaculture,2003,223:117-128.
- [12]Lee B Y,Penmant D J,Kocher T D.Identification of a sex-deter-mining region in Nile tilapia(Oreochromis niloticus)using bulkedsegregant analysis[J].International Society for Animal Genetics,Animal Genetics,2003(34):1-5.
- [13]Shirak A,Palti Y,Avner C,et al.Association between loci withdeleterious alleles and distorted sex ratios in an inbred line of tilapia(oreochromis aureus)[J].The American Genetic Association,2002(93):270-276.
- [14]Pullin R S V,Lowe-McConnell R H.The biology and culture oftilapias[M].Metro Manlia,Philippines:ICLARM,MCC,Makati,1982,314:121-124.
- [15]赵菲,张素娟,蒋胜辉.核酸电泳银染技术及干胶制备的改良[J].临床与实验病理学杂志,1999,15(5):401.
- [16]李思发.中国淡水主要养殖鱼类种质研究[M].上海科学技术出版社,1998.
- [17]岳志芹,王伟继,孔杰,等.用AFLP方法分析中国对虾抗病选育群体的遗传变异[J].水产学报,2005,29(1):13-19.
- [18]王志勇,王艺垒,林利明,等.福建官井洋大黄鱼AFLP指纹多态性研究[J].中国水产科学,2002,9(3):198-202.
- [19]张全启,徐晓斐,齐洁,等.牙鲆野生群体和养殖群体遗传多样性分析[J].中国海洋大学学报,2004,34(5):816-820.
- [20]张俊彬,黄良民.紫红笛鲷遗传多样性的AFLP分析[J].热带海洋学报,2004,23(5),50-55.
- [21]孙其信,黄铁城.小麦杂种优势群体研究:Ⅰ利用RAPD标记研究小麦品种间遗传差异[J].农业生物技术学报,1996,4(2):103-109.
- [22]李春丽.应用分子标记差异性预测作物杂种优势的研究进展[J].遗传,1997,19(1):46-48.
- [23]Melchinger A E,Lee M,Lamkey K R.Genetic diversity for restric-tion fragment length polymorphisms and heterosis for two diallel setsof maize inbreds[J].Theor Appl Gen et,1990,80:488-496.
- [24]董在杰,夏德全,吴婷婷.RAPD技术在鱼类杂种优势研究中的应用[J].中国水产科学,1999,6(1):37-40.
- [25]Bardakci F,Skibinski D O F.Application of the RAPD technique intilapia fish:species and subspecies identification[J].Heredity,1994,73:117-123.
文章评论(Comment)：
|
||||||||||||||||||
|
||||||||||||||||||