吴春晗,白洁,赵阳国,任朝萌,陈琳,孙鹏飞,李岿然

• 1:中国海洋大学环境科学与工程学院
• 2:中国海洋大学海洋环境与生态教育部重点实验室
• 3:中国海洋大学海洋生命学院

摘要(Abstract)：

本文通过分析在不同浓度普通氧化锌(ZnO)、硫酸锌(ZnSO_4)及纳米氧化锌(ZnO-NPs)胁迫下1株筛自胶州湾的好氧反硝化菌Zobellella sp. B307生长量和反硝化能力的变化,比较研究了不同类型Zn对该菌株生长及脱氮功能的影响。结果表明,ZnO-NPs对该菌株的生长有明显的持续抑制作用,显著降低其最大生长量,并对其脱氮功能产生持续抑制;ZnSO_4对菌株生长及脱氮功能前期抑制作用较强,后期抑制效应逐渐减弱;普通ZnO对菌株生长及脱氮功能并不产生显著影响。3种类型锌对该菌株的功能抑制表现为ZnO-NPs抑制作用最强,其次是ZnSO_4,普通ZnO的抑制相对较弱,产生影响差异的主要原因与这三种类型Zn化合物作用机制的不同有关。研究结果可为深入探讨不同类型的Zn污染对近海沉积物氮素循环产生的潜在影响提供依据。

关键词(KeyWords)： 锌;纳米氧化锌;好氧反硝化细菌;OD_(600)

Abstract:

Keywords:

基金项目(Foundation): 国家重点研究发展计划项目(2018YFC1407601)资助~~

作者(Author): 吴春晗,白洁,赵阳国,任朝萌,陈琳,孙鹏飞,李岿然

参考文献(References)：

• [1] Donald M.Multimedia Environmental Models:The Fugacity Approach[M].Florida,USA:Crc Press InC,2001.
• [2] 廖国礼，吴超.资源开发环境重金属污染与控制[M].长沙：中南大学出版社，2006.LIAO Guo-Li,WU Chao.Environmental Pollution and Control of Heavy Metals in Resource Development[M].Changsha:Press of Contral South University,2006.
• [3] 朱珏，黄逸强.铜、锌对细菌耐药性有促进性作用[J].湖南饲料，2014(5):21-22.ZHU Yu,HUANG Yi-Qiang.Copper and zinc promote the resistance of bacteria[J].Hunan Feed,2014(5):21-22.
• [4] Zhang W,Yu L,Hutchinson S M,et al.China’s Yangtze Estuary:I.Geomorphic influence on heavy metal accumulation in intertidal sediments[J].Geomorphology,2001,41(2-3):195-205.
• [5] 何孟常.水体沉积物重金属生物有效性及评价方法[J].环境工程学报，1998(5):9-19.HE Meng-Chang.Bioavailability and evaluation method of heavy metals in water sediments[J].Journal of Environmental Engineering,1998(5):9-19.
• [6] 李莉，梁生康，石晓勇，等.2007年环胶州湾入海河流污染状况和污染物入海通量分析[J].环境科学与管理，2009,9(6):27-32.LI Li,LIANG Sheng-Kang,SHI Xiao-Yong,et al.Analysis of the pollution situation and flux of pollutants into the sea of the Jiaozhou Bay in 2007[J].Environmental Science and Management,2009,9(6):27-32.
• [7] 李艺红.锦州湾水环境污染对沉积物微生物生态状况的影响关系研究[D].沈阳：沈阳理工大学，2014.LI Yi-Hong.Study on the Relationship Between the Water Environment Pollution of Jinzhou Bay and the Ecological Status of Sediment Microbes[D].Shenyang:Shenyang University of Science and Technology,2014.
• [8] 陈祖军，谭显英，韦鹤平.论我国海洋资源与环境的可持续发展[J].水资源保护，2001(1):7-10.CHEN Zu-Jun,TAN Xian-Ying,WEI He-Ping.On the sustainable development of China’s marine resources and environment[J].Water Resources Protection,2001(1):7-10.
• [9] 李玉，俞志明，曹西华，等.重金属在胶州湾表层沉积物中的分布与富集[J].海洋与湖沼，2005,36(6):580-589.LI Yu,YU Zhi-Ming,CAO Xi-Hua,et al.Distribution and enrichment of heavy metals in surface sediments of Jiaozhou Bay[J].Oceanologia Et Limnologia Sinica,2005,36(6):580-589.
• [10] 许腾.不同铜、铁、锌添加剂体外利用率研究[J].中国畜牧兽医，2007,34(8):22-23.XU Teng.In vitro utilization of different copper,iron and zinc additives[J].Chinese Animal Husbandry and Veterinary Medicine,2007,34(8):22-23.
• [11] Yamamoto O,Komatsu M,Sawai J,et al.Effect of lattice constant of zinc oxide on antibacterial characteristics[J].Journal of Materials Science Materials in Medicine,2004,15(8):847-851.
• [12] Ohira T,Yamamoto O,Iida Y,et al.Antibacterial activity of ZnO powder with crystallographic orientation[J].Journal of Materials Science Materials in Medicine,2008,19(3):1407-1412.
• [13] Brayner R,Ferrari-Iliou R,Brivois N,et al.Toxicological impact studies based on escherichia coli bacteria in ultrafine ZnO nanoparticles colloidal medium[J].Nano Letters,2006,6(4):866-870.
• [14] Ma T F,Chen Y P,Fang F,et al.Effects of ZnO nanoparticles on aerobic denitrifying bacteria Enterobacter cloacae strain HNR[J].Science of the Total Environment,2020(725):138284.
• [15] Zheng Xiong Su.Zinc oxide nanoparticles cause inhibition of microbial denitrification by affecting transcriptional regulation and enzyme activity[J].Environmental Science & Technology:ES&T,2014,48(5):13800-13807.
• [16] Chen N L,Feng H X,Wang Y,et al.Advances of nano-inorganic antibacterial agents loaded silver[J].Appl Chem Ind,2009,38(5):717-720.
• [17] Gui M Y,Chen Q,Ma T,et al.Effects of heavy metals on aerobic denitrification by strain Pseudomonas stutzeri PCN-1[J].Applied Microbiology & Biotechnology,2017(101):1717-1727.
• [18] Daverey A,Chen Y C,Sung S,et al.Effect of zinc on anammox activity and performance of simultaneous partial nitrification,anammox and denitrification (SNAD) process[J].Bioresource Technology,2014,165:105-110.
• [19] Justin T S,Tomas J W.Decreased astroglial cell adhesion and proliferation on zinc oxide nanoparticle polyurethane composites[J].Intermational of Nanomedicine,2008,3(4):523-531.
• [20] 李洪亮，陈玉成.污水生物处理中重金属的毒理学研究进展[J].微量元素与健康研究，2006,23(1):52-55.LI Hong-Liang,CHEN Yu-Cheng.Research progress of toxicology of heavy metals in wastewater biological treatment[J].Studies of Trace Elements and Health,2006,23(1):52-55.
• [21] Ge Y,Schimel J P,Holden P A.Evidence for negative effects of TiO2 and ZnO nanoparticles on soil bacterial communities[J].Environmental Science & Technology,2011,45:1659-1664.
• [22] Hang G,Schreiber F,Collins G,et al.Aerobic denitrification in permeable Wadden Sea sediments[J].The Intermational Society for Microbial Ecology Journal,2010(4):417-426.
• [23] FanL F,Shieh W Y,Wu W F,et al.Distribution of nitrogenous nutrients and denitrifiers strains in estuarine sediment profiles of the Tanshui River,northern Taiwan[J].Estuarine,Coastal and Shelf Science,2006,69:543-553.
• [24] 白洁，陈琳，黄潇，等.1株耐盐异养硝化-好氧反硝化菌Zobellella sp.B307的分离及脱氮特性[J].环境科学，2018,39(10):403-411.BAI-Jie,CHEN Lin,HUANG Xiao,et al.Isolation and denitrification characteristics of a salt-tolerant heterotrophic nitrification-aerobic denitrifying bacteria Zobellella sp.B307[J].Environmental Science,2018,39(10):403-411.
• [25] 国家环保局编委.水和废水监测分析方法[M].第 3 版.北京：中国环境科学出版社，1989:246-356.The Editorial Committee of the State Environmental Protection Administration.Water and Wastewater Monitoring and Analysis Methods[M].3rd edition.Beijing:China Environmental Science Press,1989:246-356.
• [26] Lindsay J A,Foster S J.Zur:A Zn2+ responsive regulatory element of Staphylococcus aureus[J].Microbiology,2001,147(5):1259-1266.
• [27] 杨旸，江雪，彭文涛，等.ZnSO4对变异链球菌生长及生物膜形成影响的研究[J].牙体牙髓牙周病学杂志，2013(10):23-24.YANG Yang,JIANG Xue,PENG Wen-Tao,et al.The effect of ZnSO4 on the growth and biofilm formation of Streptococcus mutans[J].Chinese Journal of Conservative Dentistry,2013(10):23-24.
• [28] Granger J,Ward B B.Accumulation of nitrogen oxides in copperlimited cultures of denitrifying bacteria[J].Limnol Oceanogr,2003,48:313-318.
• [29] Zheng X,Wu R,Chen Y.Effects of ZnO nanoparticles on wastewater biological nitrogen and phosphorus removal[J].Environmental Science & Technology,2011,45(7):2826-2832.
• [30] Krous S R,Blazer V S,Meade T L.Effect of acclimation time on mitrite movement across the gill epithelia of rainbow trout,the role of“chloride cell”[J].Pro Fish Cult,1982,44(2):126-130.
• [31] 喻兵权，张宏福，陆伟，等.纳米氧化锌与普通氧化锌抑菌性能差异研究[J].饲料工业，2007(24):38-41.YU Bing-Quan,ZHANG Hong-Fu,LU Wei,et al.Study on the difference between the antibacterial properties of nano zinc oxide and ordinary zinc oxide[J].Feed Industry,2007(24):38-41.
• [32] 曲敏丽，姜万超.纳米氧化锌抗菌机理探讨[J].印染助剂，2004(6):47-48.QU Min-Li,JIANG Wan-Chao.Discussion on antibacterial mechanism of nanometer zinc oxide[J].Printing and Dyeing Auxiliaries,2004(6):47-48.
• [33] 段惺，徐晓玲，黄承，等.形貌和光照条件对ZnO抗菌性能的影响[J].功能材料，2010,41(z3):496-498.DUAN Xing,XU Xiao-Ling,HUANG Cheng,et al.Effects of morphology and light conditions on antibacterial properties of ZnO[J].Journal of Functional Materials,2010,41(z3):496-498.
• [34] Dadook M,Mehrabian S,Salehi M,et al.Morphological,biochemical and molecular characterization of twelve nitrogen-fixing bacteria and their response to various zinc concentration[J].Jundishapur Journal of Microbiology,2014,7(4):e9415.
• [35] 龙新宪，刘洪彦，王艳红，等.不同Zn化合物对东南景天根圈土壤微生物特征的影响[J].农业环境科学学报，2007(6):233-240.LONG Xin-Xian,LIU Hong-Yan,WANG Yan-Hong,et al.Effects of different Zn compounds on the characteristics of soil microbes in the rhizosphere of Southeastern Jingtian[J].Journal of Agricultural Environmental Sciences,2007(6):233-240.
• [36] Navarro E,Baun A,Behra R,et al.Environmental behavior and ecotoxicity of engineered nanoparticles to algae,plants,and fungi[J].Ecotoxicology,2008,17(5):372-386.
• [37] Kumar A,Pandey A K,Singh S S,et al.Engineered ZnO and TiO2 nanoparticles induce oxidative stress and DNA damage leading to reduced viability of Escherichia coli[J].Free Radical Biology and Medicine,2011,51(10):1872-1881.
• [38] Gaballa A,Helmann J D.Identification of a zinc-specific metalloregulatory protein,zur,controlling zinc transport operons in bacillus subtilis[J].Journal of Bacteriology,1998,180(22):5815-5821.
• [39] 侯珍，陈卓，沈肇怡，等.纳米氧化锌对土壤微生物酶活性的影响[J].农业环境科学学报，2014,33(6):1153-1158.HOU Zhen,CHEN Zhuo,SHEN Zhao-Yi,et al.Effect of nanometer zinc oxide on soil microbial enzyme activity[J].Journal of Agricultural Environmental Sciences,2014,33(6):1153-1158.
• [40] 陈良辉，刘美婷，余冉.污水生物处理系统中纳米颗粒污染物的影响[J].东南大学学报(自然科学版),2014,44(3):598-604.CHEN Liang-Hui,LIU Mei-Ting,YU Ran.Effects of nano-particle pollutants in sewage biological treatment system[J].Journal of Southeast University (Natural Science Edition),2014,44(3):598-604.
• [41] Xiong A M,Jayaswal R K.Molecular characterization of a chromosomal determinant conferring resistance to zinc and cobalt ions in staphylococcus aureus[J].Journal of Bacteriology,1998,180(16):4024-4029.

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