李丽霞,赵妍,周斌,董开升,唐学玺

• 1:中国海洋大学海洋生命学院
• 2:烟台大学化学生物理工学院

摘要(Abstract)：

选择1种代表性的潮间带大型海藻鼠尾藻(Sargassum thunbergii)为试材,研究了其抗氧化酶体系对不同剂量UV-B胁迫的响应。结果显示,SOD及其CAT酶活性与UV-B辐射的剂量和时间关系密切,而POX活性与此相关不显著。各处理和对照中均未测出有效的APX活性。抗氧化剂AsA含量在低、中剂量UV-B辐射时呈增加趋势。SOD同工酶谱受到UV-B辐射的显著影响。中度剂量下CAT酶出现新的谱带(CATⅡ)。低、中剂量胁迫下POXⅡ活性有轻微增加,而在此次研究中,未能检测到APX同工酶谱带。这些结果表明鼠尾藻中SOD与CAT酶活性及AsA水平对UV-B辐射增强响应最敏感,在该藻抗紫外胁迫的应激反应体系中处于核心地位。

关键词(KeyWords)： UV-B辐射;鼠尾藻;抗氧化酶;同工酶;电泳

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30270258);; 山东省自然科学基金项目(2007ZRB01903)资助

作者(Author): 李丽霞,赵妍,周斌,董开升,唐学玺

DOI: 10.16441/j.cnki.hdxb.2009.06.017

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