循环水养殖系统中好氧反硝化细菌的分离和应用Isolation of Aerobic Denitrifying Bacteria from Recirculating Aquaculture System and Their Application
陈钊,宋协法,黄志涛,李健,董登攀,任义,江玉立
摘要(Abstract):
为了分离好氧反硝化细菌,探究其好氧反硝化过程。利用BTB培养基,从珍珠龙胆(♀Epinephelus fuscoguttatus×♂Epinephelus lanceolatu)循环水养殖系统的生物滤池中筛选出具有硝酸盐去除能力的细菌,并选择脱氮效果良好的菌株进行好氧反硝化反应器的构建,开展反硝化应用研究。本研究共分离出8株具有去除硝酸盐能力的菌株,经反硝化性能测定,都可大幅去除硝酸盐,同时也存在不同程度的氨氮和亚硝酸盐的积累;选择Z1、Z8两株脱氮效果较好的菌株进行好氧反硝化反应器的混合接种试验,结果显示反应器挂膜迅速、高效,接种2周后即达到相对稳定的水处理状态,硝酸盐去除率超过98.8%(约0.827g NO-3-N/(m2·d)),总氮去除率超过71.8%(约0.687g TN/(m2·d)),亚硝酸盐和氨氮的积累不明显,脱氮效果良好。
关键词(KeyWords): 好氧反硝化细菌;生物反应器;生物脱氮;循环水养殖
基金项目(Foundation): 国家科技支撑计划项目(2011BAD13B04);; 中国海洋大学青年教师科研专项基金项目(201413062);; 山东省博士后创新项目(201302025)资助~~
作者(Author): 陈钊,宋协法,黄志涛,李健,董登攀,任义,江玉立
DOI: 10.16441/j.cnki.hdxb.20170334
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