刘文敏,张晓娜,魏朋浩,汝少国

• 1:中国海洋大学海洋生命学院

摘要(Abstract)：

为探究双酚S(BPS)长期暴露对雌性斑马鱼的视觉毒性效应,本研究采用半静态暴露试验方法,以1、10、100和1 000μg/L BPS暴露斑马鱼胚胎至性成熟(120d),检测了BPS对雌性斑马鱼视觉行为——眼动反应和视动反应、视网膜结构及视蛋白基因(zfrho、zfblue、zfgr、zfred和zfuv)转录水平的影响。结果表明,BPS长期暴露降低了雌性斑马鱼视觉追踪能力;1 000μg/L BPS增加了视网膜及其各层厚度;BPS显著上调了眼中视蛋白基因zfuv、zfred、zfgr和zfblue的表达,增强了斑马鱼对紫外、红、绿和蓝光的光谱敏感性。本研究从分子、组织学和个体等不同水平,首次证实了BPS长期暴露能影响雌性斑马鱼的视蛋白基因表达,损伤视网膜结构,并导致其视觉行为异常,为进一步研究BPS的视觉毒性机制提供了理论基础。

关键词(KeyWords)： 双酚S;雌性斑马鱼;视觉行为;视网膜结构;视蛋白基因

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(41676100);; 中国海洋大学青年教师科研专项基金项目(20170340)资助~~

作者(Author): 刘文敏,张晓娜,魏朋浩,汝少国

DOI: 10.16441/j.cnki.hdxb.20170161

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