曾晓起,张文峰,高天翔

• 1:中国海洋大学水产学院

摘要(Abstract)：

本研究以16SrRNA和细胞色素氧化酶I(Cytochrome oxidase subunit I,COI)基因片段序列为标记,以杂色角孔海胆(Salmacis sphaeroides)为外群,基于距离法(NJ)、最大简约法(MP)和最大似然法(ML)分别构建分子系统树,对刻肋海胆属(Temnopleurus)进行了系统发育学研究。结果表明,哈氏刻肋海胆、细雕刻肋海胆、T.alexandri三者亲缘关系较近,芮氏刻肋海胆和T.michaelseni亲缘关系较近。两个基因片段的核苷酸替代速率顺序为COI>16S rRNA。以3.49%/百万年的核苷酸分歧速率应用于5种海胆的COI基因片段,推断5种海胆分化事件主要发生在约500～590万年前,为中新世晚期(Late Miocene)或上新世早期(Early Pliocene)。

关键词(KeyWords)： 刻肋海胆属;细胞色素氧化酶Ⅰ(COI);16S rRNA;系统发育

Abstract:

Keywords:

基金项目(Foundation): 公益性行业(农业)科研专项(200903005);; 国家908专项(908-01-ST12)资助

作者(Author): 曾晓起,张文峰,高天翔

DOI: 10.16441/j.cnki.hdxb.2012.06.007

参考文献(References)：

• [1]Liao Y L,Clark A M.The Echinoderms of Southern China[M].California:Science Press,1995:347-351.
• [2]张凤瀛,廖玉麟.中国动物图谱-棘皮动物[M].北京:科学出版社,1964:83-84.
• [3]Kitazawa C,Nishimura H,Yamaguchi T,et al.Novel morpho-logical traits in the early developmental stages of Temnopleurustoreumaticus[J].Biological bulletin,2009,217:215-221.
• [4]Okada K,Miyauchi H.Figures of the standard early developmentof Temnopleurus toreumaticus[J].Journal Gakugei Bull,To-kushima University,1958,8:59-71.
• [5]Osanai K.Behavior of pigment granules during early developmentin eggs of a sea urchin Temnopleurus hardwickii[J].Annual Re-port Faculty of Education Iwate University,1969,29:35-38.
• [6]Brown W M.Evolution of Animal Mitochondrial DNA[M].Sun-derland:Evolution of Genes and Proteins,1983:62-88.
• [7]Avise J C.Phylogeography[M].London:Harvard UniversityPress,2000:1-36.
• [8]Moritz C,Dowling T E,Brown W M.Evolution of animal mito-chondrial DNA:relevance for population biology and systematics[J].Annual Review of Ecology and Systematics,18:269-292.
• [9]蒙子宁,庄志猛,丁少雄,等.中国近海8种石首鱼类的线粒体16SrRNA基因序列变异及其分子系统进化[J].自然科学进展,2004,14(5):514-521.
• [10]刘晓慧,黄佳琪,周遵春,等.5种经济海胆线粒体16SrRNA基因片段的序列分析[J].水产科学,2007,26(6):331-334.
• [11]毛阳丽,蔡厚才,李成九,等.基于线粒体COI与16SrRNA基因序列探讨贻贝属的系统发育[J].南方水产,2010,6(5):27-36.
• [12]李颖,刘萍,孙慧玲,等.仿刺参(Apostichopus japonicus)mtD-NA三个基因片段的序列分析[J].海洋与湖沼,2006,37(2):143-153.
• [13]Sambrook J,Fritsch E F,Maniatis T,et al.Molecular Cloning:A Laboratory Manual[M].New York:Cold Spring Harbor La-boratory Press,1996:464-468.
• [14]Kerry L.Howell,Alex D.Rogers,Paul A.Tyler,et al.Repro-ductive isolation among morphotypes of the Atlantic seastar spe-cies Zoroaster fulgens(Asteroidea:Echinodermata)[J].MarineBiology,2004,144:977-984.
• [15]Kumar S,Tamura K,Nei M,et al.MEGA 3:integrated soft-ware for molecular evolutionary genetics analysis and sequence a-lignment[J].Briefings in Bioinformatics,2004,5(2):150-163.
• [16]Swofford D L.PAUP*Phylogenetic Analysis Using Parsimony(*and other methods),Version 4[M].Massachusetts:Sinau-er Associates,2002.
• [17]Posada D,Crandall K A.Modeltest:Testing the model of DNAsubstitution[J].Bioinformatics,1998,14:817-818.
• [18]张文峰,曾晓起,王长云.对三种刻肋海胆分类的数量分析研究[J].中国海洋大学学报:自然科学版,2011,41:169-174.
• [19]ZhengX D,WangR C,WangX F,et al.Genetic variation in pop-ulation of the common chinese cuttle fish Sepiella Maindroni(Mollusca:Cephalopoda)using allozymes and mitochondrialDNA sequence analysis[J].Journal of Shellfish Research,2001,20(3):1159-1165.
• [20]McCartney M A,Keller G,Lessios H A.Dispersal barriers intropical oceans and speciation in Atlantic and eastern pacific seaurchins of the genus Echinometra[J].Molecular Ecology,2000,9:1391-1400.
• [21]Charlotte H J,Richard B E.Phylogeny and evolution of develop-mental mode in Temnopleuridae(Echinoids)[J].Molecular Phy-logenetics and Evolution,2003,28:99-118.
• [22]Hewitt G M.Some genetic consequences of ice ages and their rolein divergence and speciation[J].Biological Journal of the Linne-an Society,1996,58(3):247-276.

文章评论(Comment)：