段妍,张秀梅,张志新

• 1:中国海洋大学水产学院
• 2:辽宁省海洋水产科学研究院

摘要(Abstract)：

以凡纳滨对虾(Litopenaeus vannamei)为研究对象,在水温(25.0±0.5)℃条件下,研究30d养殖周期内,4个梯度的溶解氧(T1:2.0±0.3、T2:4.2±0.3、T3:6.8±0.7、T4:13.6±2.1mg/L)对凡纳滨对虾生长及消化酶活性的影响。结果表明,不同水平的溶解氧对实验虾的生长和消化酶活力存在显著影响,实验结束时,T4处理组实验虾的存活率最高,达到99.48%,显著高于T1和T2处理组(P<0.05),但与T3处理无显著差异(P>0.05),低氧条件下死亡虾甲壳薄软、被残食等现象较多。养殖30d后,T1处理组实验虾平均湿重显著低于其他处理组(P<0.05),其他各处理组之间无显著差异(P<0.05)。食物转化效率、蛋白酶和脂肪酶活力均随水体溶解氧的升高而显著增加,在高氧处理组(T4)显著高于其他处理组(P<0.05),而淀粉酶活力在4个溶解氧处理组均无显著差异(P>0.05)。研究表明,低溶解氧会使凡纳滨对虾生长受到抑制、死亡率升高;而超饱和溶解氧则能够促进对虾的饵料转化效率、保证其存活率。

关键词(KeyWords)： 溶解氧;凡纳滨对虾;存活率;特定生长率;饵料转化率;消化酶

Abstract:

Keywords:

基金项目(Foundation): 公益性行业(农业)科研专项(201003068)资助

作者(Author): 段妍,张秀梅,张志新

DOI: 10.16441/j.cnki.hdxb.2013.02.002

参考文献(References)：

• [1]Wei L Z,Zhang X M,Huang G Q.Effects of limited dissolved ox-ygen supply on the growth and energy allocation of juvenile Chi-nese shrimp,Fenneropenaeus Chinensis[J].J World AquacultSoc,2009,40:483-492.
• [2]Fotheringham N,Weissberg G H.Some causes,consequences andpotential environmental impacts of oxygen depletion in the North-ern Gulf of Mexico.In:Kusakin O G[M].∥Proceedings of 11thAnnual Offshore Technology Conference.Houston:Annual Off-shore Technology Conference,1979:2205-2208.
• [3]林卫强,李适宇.夏季伶仃洋COD、DO的垂向分布及其影响因素[J].中山大学学报:自然科学版,2002,41:82-86.
• [4]罗琳,李适宇,厉红梅.夏季珠江口水域溶解氧的特征及影响因素[J].中山大学学报:自然科学版,2005,44:118-122.
• [5]Cheng W,Liu C H,Kuo C M.Effects of dissolved oxygen on he-molymph parameters of freshwater giant prawn,Macrobrachiumrosenbergii(de Man)[J].Aquaculture,2003,220:843-856.
• [6]赵慧慧,吴垠,王炳刚,等.循环养殖系统中溶氧水平对虹鳟消化酶活性及消化吸收率的影响[J].大连水产学院学报,2007,22:198-202.
• [7]Boyd C E.Pond water aeration systems[J].Aquac Eng,1998,18:9-40.
• [8]王光荣,靳巨军.高效纯氧增养石的试验与应用[J].渔业现代化,2003,3:27-28.
• [9]MacKay R D.A note on minimal levels of oxygen required tomaintain life in Penaeus schmitti.In Avault J W[M].∥Proceed-ings of the Fifth Annual Workshop of the World Mariculture Soci-ety.Charleston:Workshop of the World Mariculture Society,1974,21.
• [10]Allan G L,Maguire G B.Lethal levels of low dissolved oxygenand effects of short-term oxygen stress on subsequent growth ofjuvenile(Penaeus monodon)[J].Aquaculture,1991,94:27-37.
• [11]Yamochi S,Ariyama H,Sano M.Occurrence and hypoxic toler-ance of the juvenile Metapenaeus ensis at the mouth of the YodoRiver,Osaka[J].Fisheries Sci,1995,61:391-395.
• [12]Martinez E,Aguilar M,Trejo L,et al.Lethal low dissolved ox-ygen concentrations for postlarvae and early juvenile Penaeus se-tiferus at different salinities and pH[J].J World Aquacult Soc,1998,29:221-229.
• [13]Jiang J.Study of oxygen consumption rate,CO2exhaust,respir-atory quotient and tolerance to low dissolved oxygen in fourshrimp species[J].Journal of Zhanjiang Ocean University,1999,19:10-16.
• [14]Wu R S S,Lam P K S,Wan K L.Tolerance to,and avoidanceof,hypoxia by the penaeid shrimp(Metapenaeus ensis)[J].En-viron Pollut,2002,118:351-355.
• [15]Rosas C,Sanchez A,D′Iaz-Iglesia E,et al.Critical dissolved ox-ygen level to Penaeus setiferus and P.schmitti postlarvae(PL10-18)exposed to salinity changes[J].Aquaculture,1997,152:259–272.
• [16]Renaud M L.Detecting and avoiding oxygen deficient sea waterby brown shrimp,Penaeus aztecus(Ives),and white shrimpPenaeus setiferus(Linnaeus)[J].J Exp Mar Biol Ecol,1986,98:283 292.
• [17]Seidman E R,Lawrence A L.Growth,feed digestibility,andproximate body composition of juvenile Penaeus vannamei andPenaeus monodon grown at different dissolved oxygen levels[J].J World Aquacult Soc,1985,16:333 346.
• [18]Clark J V.Inhibition of molting in Penaeus semisulcatus(DeHaan)by long-term hypoxia[J].Aquaculture,1986,52:253254.
• [19]Ocampo L,Ezquerra J M.Digestive protease activity in juvenilefarfantepenaeus californiensis as a function of dissolved oxygenand temperature[J].Aquac Res,2002,33:1073-1080.
• [20]Li Y Q,Li J,Wang QY.The effects of dissolved oxygen concen-tration and stocking density on growth and non-specific immunityfactors in Chinese shrimp,Fenneropenaeus chinensis[J].Aqua-culture,2006,256:608 616.
• [21]Menz A,Blake B F.Experiments on the growth of Penaeus van-namei Boone[J].J Exp Mar Biol Ecol,1980,48:99-111.
• [22]陈琴,谢达祥,唐泽皓.南美白对虾仔、幼虾对盐度突变的适应性试验[J].淡水渔业,2002,32(5):11-14.
• [23]肖国强,潘鲁青,冉宪宝,等.低盐度地下卤水养殖南美白对虾的研究[J].海洋科学,2002,26(12):36-40.
• [24]臧维玲,林喜臣,戴习林,等.淡化方式与盐度对凡纳滨对虾幼虾生长的影响[J].上海水产大学学报,2003,12(4):308-312.
• [25]王芳,董双林,董少帅,等.光照周期对中国对虾(Fennero-penaeus chinensis)稚虾3种消化酶比活力昼夜变化节律的影响[J].海洋与湖沼,2003,(973增刊):36-41.
• [26]Bayer R,Riley J,Donahue D.The effect of dissolved oxygen lev-els on the weight gain and shell hardness of new-shell Americanlobster,Homarus americanus[J].J World Aquacult Soc,1998,29(4):491-493.
• [27]Ocampo Victoria L.Effect of dissolved oxygen and temperatureon the growth,respiratory metabolism and energetics of brownshrimp(Penaeus californiensis)juveniles[D].La Paz:Cent.deInvestigaciones Biologicas del Noroeste.Baja California Sur,Mexico,1998,32
• [28]Coiro L L,Poucher S L,Miller D C.Hypoxic effects on growthof Palaemonetes vulgaris larvae and other species:using constantexposure data to estimate cyclic exposure response[J].J ExpMar Biol Ecol,2000,247:243-255.
• [29]McGraw W,Teichert-Coddington D R,Rouse D B,et al.Higherminimum dissolved oxygen concentrations increase penaeid shrimpyields in earthen ponds[J].Aquaculture,2001,199:311-321.
• [30]Kramer D L.Dissolved oxygen and fish behavior[J].EnvironBiol Fish,1987,18:81-92.
• [31]Brett J R.Environmental factors and growth.In:Hoar,W S,Randall D J,Brett J R[M].∥Fish Physiology,Vol.VIII NewYork:Academic Press,1979:599-675.
• [32]Jobling M.Fish Bioenergetics[M].London:Chapman&Hall,1994.
• [33]Foss A,Vollen T,iestad V.Growth and oxygen consumption innormal and O2supersaturated water,and interactive effects of O2saturation and ammonia on growth in spotted wolfish(Anarhich-as minor Olafsen)[J].Aquaculture,2003,224:105-l16.
• [34]张沛东,张秀梅,李健.对虾的行为生态学研究进展Ⅰ.对虾的行为习性[J].应用生态学报,2006,17:127-130.
• [35]李健,孙修涛,赵法箴.水温和溶解氧含量对中国对虾摄食影响的观察[J].水产学报,1993:17(4),333-336.
• [36]Foss A,Evensen T H,iestad V.Effects of hypoxia and hyperox-ia oi1growth and food conversion efficiency in the spotted wolfshAnarhichas minor(Olafsen)[J].Aquae.Res.,2002,33:437-444.
• [37]Edsall D A,Smith C E.Performance of rainbow trout and SnakeRiver cutthroat trout reared in oxygen-supersaturated water[J].Aquaculture,1990,90:251 259.
• [38]Doulos S K,Kindschi G A.Effects of oxygen supersaturation onthe culture of cutthroat trout,Oncorhynchus clarki Richardson,and rainbow trout,Oncorhynchus mykiss Richardson[J].AquacRes,1990,21:39-46.
• [39]徐勇,曲克明,马绍赛.过饱和溶氧对大菱鲆生长及消化酶的影响[J].渔业现代化,2008,35:24-27.
• [40]Adelman I R,Smith L L.Effect of oxygen on growth and foodconversion efficiency of northern pike[J].Progr Fisher Cult,1970,32:93-96.
• [41]Fry F E J.The effect of environmental factors on the physiologyof fish.In:Hoar W S,Randall D J.[M].∥Fish Physiology,vol.6.New York:Academic Press,1971:1-98.
• [42]Brett J R.Environmental factors and growth.In:Hoar W S,Randall D J.[M].//Fish Physiology,vol.8.New York:Aca-demic,1979,599-675.
• [43]Brett J R,Blackburn J M.Oxygen requirements for growth ofyoung coho(Oncorhyncus kisutch)and sockeye(O.nerka)salm-on at 15℃[J].Can J Fish Aquat Sci,1981,38:399-404.
• [44]Buentello J A,Gatlin D M,Neill W H.Effects of water temper-ature and dissolved oxygen on daily feed consumption,feed utili-zation and growth of channel catfish(Ictalurus punctatus)[J].Aquaculture,2000,182:339-352.
• [45]Wilhelm Filhoa D,Torresa M A,Zaniboni-Filhob E,et al.Effect of different oxygen tensions on weight gain,feed conver-sion,and antioxidant status in piapara,Leporinus elongatus(Va-lenciennes,1847)[J].Aquaculture,2005,244:349-357.
• [46]Van Wormhoudt A.Variation des prot ases,des amylases et desprot ines solubles au cours du d veloppement larvaire chez Palae-mon serratus[J].Mar Biol,1973,19:245-248.
• [47]Lee P G,Smith L L,Lawrence A L.Digestive protease ofPenaeus vannamei Boone:relationship between enzyme activity,size and diet[J].Aquaculture,1984,42:225-239.
• [48]Lovett D L,Felder DL.Ontogenic change in digestive enzyme ac-tivity of larval and postlarval white shrimp Penaeus setiferus(Crustacea,Decapoda,Penaeidae)[J].Biol Bull,1990,178:144-159.
• [49]Galgani F.Regulation de l'activite des proteases digestives dePenaeus japonicus Bate en relation avec la temperature[J].JExp Mar Biol Ecol,1985,94:11-18.
• [50]VanWormhoudt A,Favrel P.Electrophoretlc characterization ofPaluemon elegans(Crustacea,Decapoda)cu-amylase system:study of amylase polymotphtsm during the mtermolt cycle[J].Camp Biochem Physiol,1988,89B:201-207.
• [51]Le Moullac G,VanWormhoudt A,Aquacop.Adaptation of di-gestive enzymes to dietary protein,carbohydrate and fibre levelsand influence of protein and carbohydrate quality in Penaeus van-namei larvae(Crustacea,Decapoda)[J].Aquat Living Resour,1994,7:203-210.
• [52]Le Moulac G,Klein B,Sellos D,et al.Adaptation of trypsin,chmotrypsin and a-amylase to casein level and protein source inPenaeus vannamei(Crustacea Decapoda)[J].J Exp Mar BiolEcol,1996,208:107-125.

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