刘彦君,王云鹏

• 1:上海交通大学医学院
• 2:中国海洋大学海洋生命学院

摘要(Abstract)：

紫杉醇是一种具有高效抗癌特性的二萜类物质,其生物合成途径一直是科学家们研究的热点。‘金锡杉'是南方红豆杉变种,相较于原种含有更加丰富的紫杉醇,因此是一种重要的遗传资源。本研究以‘金锡杉'为材料,运用生物信息学方法分析了紫杉醇合成途径中两种关键酶基因GGPPS(geranylgeranyl diphosphate synthase mRNA)和TDC1(taxadiene synthase mRNA)的序列结构,并采用半定量RT-PCR技术分析了它们的时空表达模式。生物信息学分析结果显示,基因GGPPS全长为1 889 bp,其编码的蛋白质GGPPS是一种不稳定的亲水性胞内酶。基因TDC1全长为2 700 bp,其编码的蛋白质TS是一种不稳定的脂溶性胞内酶。时间表达分析结果显示,GGPPS和TDC1在春季和夏季时期都有表达且均在春季表达量最高,该结果表明GGPPS和TDC1的表达受到季节更迭的影响。组织表达分析结果显示,GGPPS和TDC1在‘金锡杉'的树叶、树皮、树枝和果实中都有表达且两个基因在不同组织中呈现不同表达趋势。GGPPS在树叶中表达量较高,而TDC1在树皮中表达量较高,这些结果表明GGPPS和TDC1的表达同样具有组织特异性。该研究为寻找提高红豆杉中紫杉醇含量的方法提供了一定的理论基础。

关键词(KeyWords)： 紫杉醇;半定量RT-PCR;GGPPS;TDC1;表达分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(31170322)资助~~

作者(Author): 刘彦君,王云鹏

DOI: 10.16441/j.cnki.hdxb.20200076

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