陈彩芳,温海深,何峰,董双林

• 1:中国海洋大学海水养殖教育部重点实验室

摘要(Abstract)：

采用1种新的简并引物设计方法——CODEHOP法来克隆半滑舌鳎CYP17基因。该法结合NCBI、Blastp、BlockMaker、CODEHOP等生物信息学资源和DNAMAN、Oligo6.0等生物软件,程序性设计半滑舌鳎CYP17基因的CODEHOP引物,从半滑舌鳎卵巢组织中成功克隆了CYP17基因,该基因片段与其它物种的CYP17家族具有较高的同源性,经鉴定,该基因属于整个脊椎动物的CYP17基因群,尤其是鱼类亚群。实验结果表明,程序性设计的简并引物较传统简并引物特异性更高,假阳性率少,更有利于实验成功。

关键词(KeyWords)： 半滑舌鳎;简并引物;CYP17

Abstract:

Keywords:

基金项目(Foundation): 国家“十一五”科技支撑计划课题(2006BAD09A01)资助

作者(Author): 陈彩芳,温海深,何峰,董双林

DOI: 10.16441/j.cnki.hdxb.2009.06.011

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