曹建,贾子才,丛培旭,陶素媛,李兆杰,徐杰,薛长湖

• 1:中国海洋大学食品科学与工程学院

摘要(Abstract)：

利用高效液相色谱-四极杆-飞行时间串联质谱法(LC-Q-TOF)分析比较冰岛刺参(Cucumaria frondosa)和南非花刺参(Stichopus variegatus)脑苷脂的分子种组成。以氯仿-甲醇(2∶1,V/V)溶液提取,经皂化和SPE净化,在正离子模式下,通过自动二级(MS/MS)方式同时获取脑苷脂分子的一级和二级质谱图。筛取含180Da中性丢失碎片的分子,再结合二级质谱中的长链碱和脂肪酸碎片,确定每种脑苷脂分子的结构。研究表明:一次性从冰岛刺参中分析出56种脑苷脂分子,从南非花刺参中分析出109种脑苷脂分子;南非花刺参含长链碱种类较多,为15种,冰岛刺参含长链碱11种。冰岛刺参脑苷脂中鞘氨醇型长链碱占91.84%,南非花刺参脑苷脂中鞘氨醇型长链碱占85.97%;脂肪酸的组成均为17~25碳的饱和或单不饱和脂肪酸,且以24碳脂肪酸为主。冰岛刺参脑苷脂中2-羟基脂肪酸占总脂肪酸的33.50%,饱和脂肪酸∶单不饱和脂肪酸≈2∶3;南非花刺参脑苷脂中2-羟基脂肪酸占总脂肪酸的76.73%,饱和脂肪酸∶单不饱和脂肪酸≈2∶1。研究结果显示,在抑制肿瘤细胞增长、预防癌症方面南非花刺参脑苷脂较冰岛刺参脑苷脂可能具有更好的生物活性。本研究为不同海参脑苷脂的活性研究和营养评价提供了参考与借鉴。

关键词(KeyWords)： 脑苷脂;高效液相色谱-四极杆-飞行时间串联质谱法;冰岛刺参;南非花刺参;分子种

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31201329)资助~~

作者(Author): 曹建,贾子才,丛培旭,陶素媛,李兆杰,徐杰,薛长湖

DOI: 10.16441/j.cnki.hdxb.20150196

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