黄有松,徐东晖,陈洪举,张寰,林森杰,刘光兴

• 1:中国海洋大学环境科学与工程学院
• 2:中国海洋大学海洋环境学院
• 3:中国海洋大学海洋环境与生态教育部重点实验室
• 4:Department of Marine Sciences University of Connecticut

摘要(Abstract)：

浮游桡足类是海洋初级生产力和高级营养级之间能量传递的重要中介,对自然海区桡足类食物组成的研究是深入了解其在海洋食物网所处生态地位的关键。然而,由于研究方法的局限,目前很难获取桡足类准确的现场摄食信息。本文用分子生物学方法研究了黄河口邻近水域中华哲水蚤的现场食物组成,对现场采集固定的中华哲水蚤样品进行清洗、去除附肢、镜检等处理,研磨匀浆后提取总基因组DNA。基于对以此DNA为模板扩增出的非桡足类核糖体小亚基基因(non-copepod 18SrDNA)序列的分析,本文共检测出属于8个门的中华哲水蚤的潜在食物种类。研究结果表明,PCR的分子生物学技术在桡足类摄食研究中具有强大的效用,同时,诸多潜在食物类群的发现也意味着利用分子生物学方法对桡足类及其它海洋生物现场食物组成进行深入系统研究,将有利于揭示海洋生态系统结构与功能的真实状态,促进海洋生态系统物质循环和能量流动的研究。

关键词(KeyWords)： 中华哲水蚤;现场食物;18SrDNA;黄河口;分子生物学

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(41076085;40876066)资助

作者(Author): 黄有松,徐东晖,陈洪举,张寰,林森杰,刘光兴

DOI: 10.16441/j.cnki.hdxb.2014.03.012

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