盐胁迫对间歇增氧垂直流人工湿地脱氮性能及微生物群落影响Effects of Salinity on Nitrogen Removal Performance and Microbial Community of Intermittent Aeration Vertical Flow Constructed Wetland
宋利国,宋维星,王宜东,周衍平,洪波,季军远
摘要(Abstract):
利用新型间歇增氧垂直流人工湿地,揭示了不同进水NH~+_4-N浓度下盐度提升对湿地脱氮性能及微生物群落的影响。试验结果表明:间歇增氧垂直流人工湿地在盐胁迫下的脱氮性能明显优于传统垂直流人工湿地,高盐(2.0%盐度)对TN去除性能影响较大,对COD去除性能影响相对较小。进水NH~+_4-N浓度为40 mg/L时,当盐度由0%提升至2.0%,出水NH~+_4-N浓度由3.88 mg/L增至12.44 mg/L,NH~+_4-N去除率由90.3%降至68.9%;出水NO~-_2-N浓度在1.09~1.21 mg/L,波动较小,出水NO~-_3-N浓度由5.19 mg/L降至3.81 mg/L;TN浓度由10.28 mg/L增至17.40 mg/L,TN去除率由74.3%降至56.5%;出水COD浓度由19.8 mg/L增至37.0 mg/L,COD去除率由89.5%降至81.5%。0.5%盐度下湿地微生物丰富度最高,且各盐度条件下曝气底部填料区微生物丰富度均高于湿地植物沙土区。随着湿地微生物对盐胁迫的逐步适应,微生物多样性初步恢复至无盐工况水平。盐度对人工湿地系统微生物菌群分布及其多样性产生显著影响,随着盐度从0.0%提升至2.0%,具有亚硝化功能的Nitrosomonas和具有硝化功能的Nitrospiraceae的丰度分别由2.5%、1.7%降至0.5%、0.3%,而具有反硝化功能的Thiobacillus和Denitratisoma平均丰度较高(分别为11.6%、5.4%)且保持相对稳定,表明其对盐胁迫具有较好的耐受性。此研究结果可为揭示盐度对人工湿地脱氮影响及推进间歇增氧垂直流人工湿地应用提供参考。
关键词(KeyWords): 人工湿地;间歇增氧;盐度;脱氮;微生物群落
基金项目(Foundation): 国家自然科学基金项目(51408570,51978636);; 中央高校基本科研业务费专项(201964005)资助~~
作者(Author): 宋利国,宋维星,王宜东,周衍平,洪波,季军远
DOI: 10.16441/j.cnki.hdxb.20210032
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