武原原,李姗姗,吴淑妍,潘云浩,于娜玲,赵长坤,高孟春

• 1:中国海洋大学海洋环境与生态教育部重点实验室
• 2:中国海洋大学环境科学与工程学院

摘要(Abstract)：

本文研究了10 mg/L的Ni~(2+)和5 mg/L的金霉素(CTC)对序批式反应器(SBR)性能、微生物酶活性和微生物群落的影响。结果表明,COD去除率在反应器整个运行过程中始终保持在90%以上。10 mg/L Ni~(2+)单独存在及其与5 mg/L CTC共存均能导致氮去除率的下降,且在二者共存时下降程度最大,表明Ni~(2+)和CTC共存对氮的去除呈现协同抑制作用。随着进水中Ni~(2+)和CTC的加入,活性污泥的耗氧速率、硝化和反硝化速率均有所降低。脱氢酶活性以及与脱氮相关的微生物酶活性的变化趋势与耗氧速率和相应的硝化反硝化速率的变化趋势保持一致,均出现了一定程度的降低。Ni~(2+)和CTC共存对硝化反硝化活性呈现协同抑制作用,协同抑制作用主要依赖于Ni~(2+)的含量。Ni~(2+)和CTC共存对活性污泥中活性氧(ROS)的产生和乳酸脱氢酶(LDH)的释放呈现协同促进作用,可以诱导活性污泥细胞内氧化应激以及细胞损伤。长期暴露条件下添加10 mg/L Ni~(2+)和5 mg/L CTC会降低微生物群落的丰富度和多样性。Ni~(2+)和CTC的存在显著抑制了活性污泥中硝化菌(Nitrosomonas、Nitrospira)和反硝化菌(Thauera、Longilinea、Denitratisoma和Anaerolinea)的相对丰度,从而抑制了SBR的脱氮性能。

关键词(KeyWords)： Ni~(2+);金霉素;微生物酶活性;微生物群落;序批式反应器

Abstract:

Keywords:

基金项目(Foundation): 中央高校基本科研业务费专项(201964003)资助~~

作者(Author): 武原原,李姗姗,吴淑妍,潘云浩,于娜玲,赵长坤,高孟春

DOI: 10.16441/j.cnki.hdxb.20200367

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