龙须菜新品系ZC的生长和生理生化参数的测定及其SCAR标记的开发The Determination of Growth,Physiological,Biochemical Parameters and the Development of SCAR Marker of the New Strain ZC of Gracilariopsis lemaneiformis(Rhodophyta)
王津果,隋正红,周伟,常连鹏,张淑,马金华,张学成
摘要(Abstract):
对龙须菜新品系ZC与栽培品系981、07-2及湛山湾野生四分孢子体的生长速度和总蛋白质含量等生理生化参数进行比较,从而为新品系ZC在栽培产业的开发利用提供理论支持。结果显示,ZC的线性生长速度高达9.89mm/d,比栽培品系981、07-2分别提高16.6%、51.7%,表明ZC相比现有的栽培品系具备较高的生长速度。藻红蛋白含量与981无明显不同,但显著低于07-2,叶绿素a含量则显著低于2个栽培品系;其总蛋白质含量7.21mg/g显著高于其他3个龙须菜材料,可作为一种提供更高蛋白质来源的鲍鱼饵料使用。在光合放氧和呼吸耗氧速率方面,ZC并未与其他龙须菜材料表现出显著差异。另外,由于ZC在外观形态上与栽培品系很难区分,本实验通过分析上述4种龙须菜的RSAP指纹图谱,筛选获得了ZC的特异条带,并将其转化为稳定可靠、特异性高的SCAR标记,用以鉴定龙须菜新品系。
关键词(KeyWords): 龙须菜;生长速度;总蛋白质含量;生理生化参数;SCAR
基金项目(Foundation): “十二五”农村领域国家科技计划项目(2012AA10A411);; 农业部公益性项目(200903030)资助
作者(Author): 王津果,隋正红,周伟,常连鹏,张淑,马金华,张学成
DOI: 10.16441/j.cnki.hdxb.2014.11.006
参考文献(References):
- [1]McHugh D J.Worldwide distribution of commercial resources of seaweeds including Gelidium[C].International Workshop on Gelidium[s.1.].Springer,1991.
- [2]Marinho-Soriano E.Agar polysaccharides from Gracilariaspecies(Rhodophyta,Gracilariaceae)[J].Journal of Biotechnology,2001,89(1):81-84.
- [3]刘朝阳,孙晓庆.龙须菜的生物学作用及应用前景[J].养殖与饲料,2007,5(27):55-58.
- [4]Zou D H,Xia J R,Yang Y F.Photosynthetic use of exogenous inorganic carbon in the agarophyte Gracilaria lemaneiformis(Rhodophyta)[J].Aquaculture,2004,237(1):421-431.
- [5]张学成,费修绠,王广策,等.江蓠属海藻龙须菜的基础研究与大规模栽培[J].中国海洋大学学报:自然科学版,2009,39(5):947-954.
- [6]Tseng C K.Algal biotechnology industry and research activities in China[J].Journal of Applied Phycology,2001,13(4):375-380.
- [7]Li Ren-Zhi,Chong Ren-Yi,Meng Zhao-Cai.A preliminary study of raft cultivation of Gracilaria verrucosa and Gracilaria sjoestedtii[C].[s.1.]:Eleventh International Seaweed Symposium,1984.
- [8]Fei Xiugeng and Zhang Xuecheng.A research on the transplant of Gracilaria lemaneiformis[C].[s.1.]:A Presentation at 4th International Phycological Congress,1991.
- [9]陈伟洲.龙须菜海水栽培技术[J].科学养鱼,2006(12):38-39.
- [10]严志洪.龙须菜南移栽培技术[J].中国水产,2003(8):62-63.
- [11]陈伟洲,徐涤,王亮根,等.两个龙须菜新品系经济性状及琼胶特性的初步研究[J].中国海洋大学学报:自然科学版,2009,39(3):437-442.
- [12]Santelices B.Seaweed research and utilization in Chile:Moving into a new phase[C].Valdivia:Fifteenth International Seaweed Symposium,1996.
- [13]Meneses I,Santelices B.Strain selection and genetic variation in Gracilaria chilensis(Gracilariales,Rhodophyta)[J].Journal of Applied Phycology,1999,11(3):241-246.
- [14]Buschmann A H,Hernández-González M C,Astudiillo C,et al.Seaweed cultivation,product development and integrated aquaculture studies in Chile[J].World Aquaculture-Bation Rouge,2005,36(3):51.
- [15]孟琳,徐涤,陈伟洲,等.龙须菜新品系07-2的筛选及性状分析[J].中国海洋大学学报:自然科学版,2009,39(3):94-98.
- [16]Mikhailova N A,Gracheva Y A,Backeljau T,et al.A potential species-specific molecular marker suggests interspecific hybridization between sibling species Littorina arcana and L.saxatilis(Mollusca,Caenogastropoda)in natural populations[J].Genetica,2009,137(3):333-340.
- [17]杜晓华,王得元,巩振辉.一种新型DNA标记技术-限制性位点扩增多态性(RSAP)的建立与优化[J].西北农林科技大学学报:自然科学版,2006,34(9):45-49.
- [18]张学成,秦松,马家海,等.海藻遗传学[M].北京:中国农业出版社,2005:239-241.
- [19]Pflugmacher S,Steinberg C.Activity of phase I and phase II detoxification enzymes in aquatic macrophytes[J].Angewandte Botanik,1997,71(5-6):144-146.
- [20]程晓杰,任雪莹,刘滨,等.龙须菜数量性状及AFLP分析[J].武汉大学学报:理学版,2008,54(6):739-744.
- [21]林贞贤,宫相忠,李大鹏.光照和营养盐胁迫对龙须菜生长及生化组成的影响[J].海洋科学,2007,31(11):22-26.
- [22]达维斯C J.海洋植物学[M].厦门:厦门大学出版社,1989.
- [23]Moran R.Formulae for determination of chlorophyllous pigments extracted with N,N-dimethylformamide[J].Plant Physiology,1982,69(6):1376-1381.
- [24]陈毓全.生物化学实验方法和技术[M].北京:科学出版社,2002.
- [25]库姆斯J,霍尔D,朗S P,等.生物生产力和光合作用测定技术[M].北京:科学出版社,1986.
- [26]丁弘叶.青岛野生种群不同倍性龙须菜AFLP遗传多样性分析以及与性别相关SCAR标记的开发与验证[D].青岛:中国海洋大学,2011.
- [27]Kursar T A,van der Meer J,Alberte R S.Light-Harvesting System of the Red AlgaGracilaria tikvahiae I.Biochemical Analyses of Pigment Mutations[J].Plant Physiology,1983,73(2):353-360.
- [28]Xia J R,Li Y J,Lu J,et al.Effects of copper and cadmium on growth,Photosynthesis,and pigment content in Gracilaria lemaneiformis[J].Bulletin of Environmental Contamination and Toxicology,2004,73(6):979-986.
- [29]Rodrigo M,Robaina R R.Stress tolerance of photosynthesis in sporelings of the red algaGrateloupia doryphora compared to that of Stage III thalli[J].Marine Biology,1997,128(4):689-694.
- [30]颜昌宙,曾阿妍,金相灿,等.不同浓度氨氮对轮叶黑藻的生理影响[J].生态学报,2007,27(1):1050-1059.
- [31]Korbee N,Figueroa F L,Aguilera J.Effect of light quality on the accumulation of photosynthetic pigments,proteins and mycosporine-like amino acids in the red alga Porphyra leucosticta(Bangiales,Rhodophyta)[J].Journal of Photochemistry and Photobiology B:Biology,2005,80(2):71-78.
- [32]Ryder E,Nelson S G,McKeon C,et al.Effect of water motion on the cultivation of the economic seaweed Gracilaria parvispora(Rhodophyta)on Molokai,Hawaii[J].Aquaculture,2004,238(1):207-219.
- [33]郭翠,陈伟洲,曹会彬,等.不同龙须菜品系在高温胁迫下的生理响应比较[J].南方水产科学,2011,7(3):14-19.
- [34]Gao K S,Xu J T.Effects of solar UV radiation on diurnal photosynthetic performance and growth of Gracilaria lemaneiformis(Rhodophyta)[J].European Journal of Phycology,2008,43(3):297-307.
- [35]Yu J,Yang Y F.Physiological and biochemical response of seaweed Gracilaria lemaneiformis to concentration changes of N and P[J].Journal of Experimental Marine Biology and Ecology,2008,367(2):142-148.
- [36]Juntian Xu,Kunshan Gao.Growth,pigments,UV-absorbing compounds and agar yield of the economic red seaweed Gracilaria lemaneiformis(Rhodophyta)grown at different depths in the coastal waters of the South China Sea[J].Journal of Applied Phycology,2007,20(5):681-686.
- [37]Han T J,Kang S H,Park J S,et al.Physiological responses of Ulva pertusa and U.armoricanato copper exposure[J].Aquatic Toxicology,2008,86(2):176-184.
- [38]Wang Zhiyuan,Wang Guangce,Niu Jianfeng,et al.Optimization of conditions for tetraspore release and assessment of photosynthetic activities for different generation branches of Gracilaria lemaneiformis Bory[J].Chinese Journal of Oceanology and Limnology,2010,28(4):738-748.
- [39]余杰,王欣,陈美珍,等.潮汕沿海龙须菜的营养成分和多糖组成分析[J].食品科学,2006,27(1):93-97.
- [40]刘静雯,董双林.光照和温度对细基江蓠繁枝变型的生长及生化组成影响[J].青岛海洋大学学报:自然科学版,2001,31(3):332-338.
- [41]周峙苗,何清,马晓宇.东海红藻龙须菜的营养成分分析及评价[J].食品科学,2010,31(9):284-288.
- [42]Dromgoole,F I.The effects of pH and inorganic carbon on photosynthesis and dark respiration of Carpophyllum(Fucales,phaeophyceae)[J].Aquatic Botany,1978,4:11-22.
- [43]Gao K,Aruga Y,Asada K,et al.Influence of enhanced CO2on growth and photosynthesis of the red algae Gracilaria sp.and G.chilensis[J].Journal of Applied Phycology,1993,5(6):563-571.
- [44]García-Sánchez M J,Fernández J A,Niell X.Effect of inorganic carbon supply on the photosynthetic physiology of Gracilaria tenuistipitata[J].Planta,1994,194(1):55-61.
- [45]Menéndez M,Martínez M,Comín F A.A comparative study of the effect of pH and inorganic carbon resources on the photosynthesis of three floating macroalgae species of a Mediterranean coastal lagoon[J].Journal of Experimental Marine Biology and Ecology,2001,256(1):123-136.
- [46]Beer S,Levy I.Effects of photon fluence rate and light spectrum composition on growth,photosynthesis and pigment relations in Gracilaria sp.[J].Journal of Phycology,1983,19(4):516-522.
- [47]García-Sánchez M J,Fernández J A,Niell F X.Biochemical and physiological responses of Gracilaria tenuistipitata uuder two different nitrogen treatments[J].Physiologia Plantarum,1993,88(4):631-637.
- [48]Dawes C J,Chen C P,Jewett-Smith J,et al.Effect of phosphate and ammonium levels on photosynthetic and respiratory responses of the red algaGracilaria verrucosa[J].Marine Biology,1984,78(3):325-328.
- [49]李效尊,袁晓君,蒋苏,等.黄瓜序列特征性扩增区域标记(SCAR)的开发[J].分子植物育种,2007,5(3):393-402.
- [50]Paran I,Michelmore R W.Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce[J].Theoretical and Applied Genetics,1993,85(8):985-993.
- [51]Horejsi T,Box J M,Staub J E.Efficiency of randomly amplified polymorphic DNA to sequence characterized amplified region marker conversion and their comparative polymerase chain reaction sensitivity in cucumber[J].Journal of the American Society for Horticultural Science,1999,124(2):128-135.
- [52]Robbins M D.Molecular Marker Development,QTL Pyramiding,and Comparative Analysis of Phenotypic and Marker-Assisted Selection in Cucumber[M].Madison:University of Wisconsin,2006.
文章评论(Comment):
|
||||||||||||||||||
|
||||||||||||||||||