马嘉忆,汪波,丁晖,韦存,刘梦然,包振民,胡景杰

• 1:中国海洋大学三亚海洋研究院海南省热带水产种质重点实验室
• 2:中国海洋大学海洋生物遗传学与育种教育部重点实验室

摘要(Abstract)：

为了探究β-类胡萝卜素裂解加氧酶(Beta-carotene oxygenase family, BCO)家族在东星斑(Plectropomus leopardus)体色形成中的作用。本研究在东星斑基因组中鉴定得到4个BCO家族基因，通过生物信息学方法对该家族基因的系统进化、基因结构、理化性质、蛋白结构以及保守性进行预测和分析。结果显示东星斑4个BCO基因都包含一个主要的RPE65功能域，并且其二级结构、超二级结构和三级结构都具有高度保守性。定量分析结果表明Bco2l和Bco2b在类胡萝卜素的主要代谢组织肝脏中有较高的表达，Bco1和Bco2l在肠道中表达较高。另外，红、黑体色差异个体中BCO基因的mRNA表达水平在多个组织中存在显著差异，其中Bco2l的表达量和东星斑红色体色具有较高的相关性，表明Bco2l可能是影响东星斑体色的关键调控基因。

关键词(KeyWords)： 东星斑;类胡萝卜素;体色;BCO基因家族;表达差异

Abstract:

Keywords:

基金项目(Foundation): 海南省重点研究发展项目(ZDYF2021XDNY133);; 三亚崖州湾科技城管理局项目(SKJC-KJ-2019 KY01,SKJC-2020-02-009)资助~~

作者(Author): 马嘉忆,汪波,丁晖,韦存,刘梦然,包振民,胡景杰

DOI: 10.16441/j.cnki.hdxb.20220055

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