高岩,王敬东,张学成

• 1:中国海洋大学海洋生命学院
• 2:青岛市立医院肾内科
• 3:青岛市中心医院ICU

摘要(Abstract)：

观察核因子kB受体激活物配体(RANKL)对大鼠血管平滑肌细胞体外钙化的调节作用,以及转鲑鱼降钙素基因酵母对RANKL调节作用的影响。体外培养大鼠血管平滑肌细胞,建立体外血管平滑肌细胞钙化模型,在培养介质中分别加入RANKL及转鲑鱼降钙素基因酵母蛋白上清液,观察细胞钙沉积、细胞碱性磷酸酶活性及骨钙素的变化情况。结果表明,RANKL促进钙化培养的大鼠血管平滑肌细胞的钙沉积、细胞碱性磷酸酶活性及骨钙素的表达,而转鲑鱼降钙素基因酵母能够抑制RANKL的促钙化作用。RANKL可能通过增加细胞碱性磷酸酶活性及骨钙素的表达从而促进血管平滑肌细胞向成骨细胞转化;转鲑鱼降钙素基因酵母可能通过OPG/RANKL/RANK途径抑制血管平滑肌细胞钙化。

关键词(KeyWords)： 钙化;血管平滑肌细胞;RANKL;转鲑鱼降钙素基因酵母

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展计划项目(819-04-10)资助

作者(Author): 高岩,王敬东,张学成

DOI: 10.16441/j.cnki.hdxb.2009.06.016

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