杨泽,张赢月,杨金鑫,徐涤

• 1:中国海洋大学海洋生命学院海洋生物遗传学与育种教育部重点实验室

摘要(Abstract)：

为筛选龙须菜5个枝叶状生活史阶段藻体中稳定表达的内参基因，本研究对9个候选内参基因，包括18S rRNA、28S rRNA、GAPDH、ACT、EF1、APC、PEB、rbcL和COX在具有世代交替生活史的产琼胶红藻龙须菜(Gracilariopsis lemaneiformis)不同生活史阶段中的表达稳定性进行评价，拷贝数分析结果表明，这些候选管家基因在不同倍性的藻体间表达差异最大，其次是雌雄配子体间的表达差异。geNorm和Normfinder软件对全部样品进行分析，发现结果与使用拷贝数的分析结果总体一致。结果表明，18S rRNA和28S rRNA比较适合作为二倍四分孢子体研究的内参基因；EF1和28S rRNA适合作为单倍体雌、雄配子体研究的内参基因；而针对5种生活史阶段藻体间的研究时，推荐28S rRNA和ACT为最适内参基因。

关键词(KeyWords)： 龙须菜;生活史阶段;实时荧光定量PCR;内参基因

Abstract:

Keywords:

基金项目(Foundation): 宁波市重大科技专项(2019B10009);; 现代农业产业技术体系专项(CARS-50)资助~~

作者(Author): 杨泽,张赢月,杨金鑫,徐涤

DOI: 10.16441/j.cnki.hdxb.20210312

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