孙琰,刘翠,池姗,唐贤明,陈傅晓,刘涛

• 1:中国海洋大学海洋生命学院
• 2:海南省水产研究所

摘要(Abstract)：

通过PCR扩增基因片段的方法测定了红翎菜科琼枝属(Betaphycus)、卡帕藻属(Kappaphycus)和麒麟菜属(Eucheuma)4种海藻共19株个体的核糖体基因转录间隔区(ITS)(含5.8SrDNA)和核酮糖-1,5-二磷酸羧化酶/加氧酶基因大亚基(rbcL)全长基因序列。其中rbcL全长序列为首次测定,为从蛋白质水平探讨红翎菜科分子系统进化提供了可靠的保证。琼枝属、卡帕藻属、麒麟菜属ITS序列长度分别为1 024、629~669、1 001bp,GC含量在45.6%~52%之间;rbcL序列长度均为1 467bp,GC含量在37.1%~37.6%之间。结合GenBank中现有的红翎菜科海藻ITS和rbcL序列进行系统进化分析,2个片段聚类结果均明显的将所有样品分为琼枝属、卡帕藻属和麒麟菜属3大分支,表现出明显的属间差异。本研究的11株长心卡帕藻根据ITS序列差异分成明显2种类型,推测这2种类型长心卡帕藻的ITS序列差异与其地理环境、无性繁殖时间(代数)和藻体颜色无关。

关键词(KeyWords)： 麒麟菜属;卡帕藻属;琼枝属;ITS;rbcL;系统发育

Abstract:

Keywords:

基金项目(Foundation): 公益性行业科研专项经费项目(201303047);; 海南省科学事业费项目(11-20410-0015);; 高校基本科研业务费项目(201362040)资助

作者(Author): 孙琰,刘翠,池姗,唐贤明,陈傅晓,刘涛

DOI: 10.16441/j.cnki.hdxb.2014.12.008

参考文献(References)：

• [1]夏邦美,张峻甫.中国海藻志[M].北京:科学出版社,1999:115-132.
• [2]Lluisma A O,Ragan M A.Relationships among Eucheuma denticulatum,Eucheuma isiforme,and Kappaphycus alvarezii(Gigartinales,Rhodophyta)based on nuclear ssu-rRNA gene sequences[J].Journal of Applied Phycology,1995,7:471-477.
• [3]Conklin E J,Smith J E.Abundance and spread of the invasive red algae,Kappaphycus spp.,in Kane′ohe Bay,Hawai'i and an experimental assessment of management options[J].Biological Invasions,2005,7:1029-1039.
• [4]Zuccarello G C,Critchley A T,Smith J E,et al.Systematics and genetic variation in commercial Kappaphycusand Eucheuma(Solieriaceae,Rhodophyta)[J].Journal of Applied Phycology,2006,18:643-651.
• [5]刘晨临,黄晓航,刘建国.4株形态不同的长心卡帕藻(Kappaphycus alvarezii)的初步ISSR分析[J].海洋科学,2009,33(4):50-53.
• [6]赵素芬,张婷,应成琦,等.长心卡帕藻RAPD-PCR反应体系的正交优化研究[J].生物技术通报,2008(4):161-165.
• [7]Yoon H S,Lee J Y,Boo S M,et al.Phylogeny of Alariaceae,Laminariaceae,and Lessoniaceae(Phaeophyceae),based on plastid-encoded RuBisCo Spacer and nuclear-encoded ITS sequence comparisons[J].Molecular Phylogenetics and Evolution,2001,21(2):231-243.
• [8]Sherwood A R,Presting G G.Characterizing green algal biogiversity of Hawaiian reef and estuarine communities:expansion of the sequence diversity assessment framework to distinguish native from alien genotypes[R].USA:University of Hawaii,2007.
• [9]Lane C E,Lindstrom S C,Saunders G W.A molecular assessment of northeast Pacific Alaria species(Laminariales,Phaeophyceae)with reference to the utility of DNA barcoding[J].Molecular Phylogenetics and Evolution,2007,44:634-648.
• [10]Conklin K Y,Kurihara A,Sherwood A R.A molecular method for identification of the morphologically plastic invasive algal genera Eucheuma and Kappaphycus(Rhodophyta,Gigartinales)in Hawaii[J].Journal of Applied Phycology,2009,21(6):691-699.
• [11]Yang E C,Kim M S,Geraldiom P J L,et al.Mitochondrial cox1and plastid rbcL genes of Gracilaria vermiculophylla(Gracilariaceae,Rhodophyta)[J].Journal of Applied Phycology,2008,20:161-168.
• [12]Fredericq S,Freshwater D W,Hommersand M H.Observations on the phylogenetic systematics and biogeography of the Solieriaceae(Gigartinales,Rhodophyta)inferred fromrbcL sequences and morphological evidence[J].Hydrobiologia,1999,398:25-38.
• [13]孙晓宇,罗丹,赵翠,等.不同保存条件下五种大型海藻的DNA提取和PCR分析[J].分子植物育种:网络版,2011,9:1680-1691.http:∥www.biopublisher.cn/index.php/mpb/article/html/569.
• [14]Kyosuke N,Atsushi K,Yusho A.Confirmation of cultivated Porphyra tenera(Bangiales,Rhodophyta)by polymerase chain reaction restriction fragment length polymorphism analyses of the plastid and nuclear DNA[J].Phycological Research,2005,53(4):296-302.
• [15]Freshwater D W,Rueness J.Phylogenetic relationships of some European Gelidium(Gelidiales,Rhodophyta)species,based on rbcL nucleotide sequence analysis[J].Phycologia,1994,33(3):187-194.
• [16]Tamura K,Dudley J,Nei M,et al.MEGA4:Molecular evolutionary genetics analysis(MEGA)software version 4.0[J].Molecular Biology and Evolution,2007,24:1596-1599.
• [17]Swofford D L.PAUP*:Phylogenetic analysis using parsimony(and other methods)[M].Version 4.Sunderland,Massachusetts:Sinauer Associates,1998.
• [18]Stamatakis A.RAxML-VI-HPC:maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models[J].Bioinformatics,2006,22:2688-2690.
• [19]Zhao S F,Peimin H E.Molecular identification based on ITS sequences for Kappaphycus and Eucheumacultivated in China[J].Chinese Journal of Oceanology and Limnology,2011,29:1287-1296.
• [20]Brodie J,Mortensen A M,Ramirez M E,et al.Making the links:towards a global taxonomy for the red algal genus Porphyra(Bangiales,Rhodophyta)[J].Journal of Applied Phycology,2008,20(5):939-949.

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