王雨,潘进芬,魏铭,王晓冰

• 1:中国海洋大学环境科学与工程学院
• 2:中国海洋大学海洋环境与生态教育部重点实验室

摘要(Abstract)：

为了解DOM与AgNPs的相互作用及其对AgNPs毒性的影响,本文以菲律宾蛤仔为受试生物、以水环境广泛存在腐殖酸(Humic acid, HA)为代表性有机质。通过贝类体内抗氧化系统的生物标志物(过氧化氢酶(Catalase, CAT)、超氧化物歧化酶(Superoxide dismutase, SOD)、金属硫蛋白(Metallothionein, MT)和丙二醛(Malondialdehyde, MDA))的响应,研究了HA对AgNPs生物效应的影响。结果表明,HA显著增加了AgNPs的稳定性,抑制了AgNPs的聚集。AgNPs(20μg·L~(-1))暴露3 d后,蛤仔鳃组织中Ag的蓄积显著上升,并随着时间的延长不断升高。AgNPs暴露第3、7天,不同浓度的HA条件下,鳃组织中Ag的蓄积无显著差异,但显著地低于无HA存在的AgNPs暴露组。CAT活性和MDA含量仅在无HA存在的AgNPs暴露组出现显著升高,其余各组之间无显著差异。表征DNA损伤程度的Olive尾矩(OTM)和尾长(TL)在无HA存在的AgNPs暴露组中随时间的延长不断上升。HA显著降低了AgNPs的对鳃组织细胞的遗传毒性。结果表明,HA通过增加AgNPs在介质中的稳定性而显著地抑制了鳃组织对Ag的蓄积,从而减弱了AgNPs对鳃组织的氧化胁迫和遗传毒性。结果证明了有机质含量等环境理化条件对纳米毒性存在显著影响。

关键词(KeyWords)： 纳米银颗粒;遗传毒性;菲律宾蛤仔;氧化胁迫;腐殖酸

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(41276104);; 国家海洋局公益专项(201505034-2)资助~~

作者(Author): 王雨,潘进芬,魏铭,王晓冰

参考文献(References)：

• [1] Ward C S,Pan J F,Colman B P,et al.Conserved microbial toxicity responses for acute and chronic silver nanoparticle treatments in wetland mesocosms[J].Environmental Science & Technology,2019,53(6):3268-3276.
• [2] Sung J H,Ji J H,Park J D,et al.Subchronic inhalation toxicity of silver nanoparticles[J].Toxicological Sciences,2009,108(2):452-61.
• [3] Aouini F,Trombini C,Sendra M,et al.Biochemical response of the clam Ruditapes philippinarum to silver (AgD and AgNPs) exposure and application of an integrated biomarker response approach[J].Marine Environmental Research,2019,152:104783.
• [4] Zhang T,Pan J F,Hunt D E,et al.Organic matter modifies biochemical but not most behavioral responses of the clam Ruditapes philippinarum to nanosilver exposure[J].Marine Environmental Research,2018,133:105-113.
• [5] Gunsolus I L,Mousavi M P S,Hussein K,et al.Effects of humic and fulvic acids on silver nanoparticle stability,dissolution,and toxicity[J].Environmental Science & Technology,2015,49(13):8078-8086.
• [6] Zhang T,Lu D,Zeng L,et al.Role of secondary particle formation in the persistence of silver nanoparticles in humic acid containing water under light irradiation[J].Environmental Science & Technology,2017,51(24):14164-14172.
• [7] Yang Y,Xu S,Xu G,et al.Effects of ionic strength on physicochemical properties and toxicity of silver nanoparticles[J].Science of the Total Environment,2018,647:1088-1096.
• [8] Angel B M,Batley G E,Jarolimek C V,et al.The impact of size on the fate and toxicity of nanoparticulate silver in aquatic systems[J].Chemosphere,2013,93(2):359-365.
• [9] Singh N P,Mccoy M T,Tice R R,et al.A simple technique for quantitation of low levels of DNA damage in individual cells[J].Experimental Cell Research,1988,175(1):184-191.
• [10] Croteau M N,Dybowska A D,Luoma S N,et al.A novel approach reveals that zinc oxide nanoparticles are bioavailable and toxic after dietary exposures[J].Nanotoxicology,2011,5(1):79-90.
• [11] Pan J F,Buffet P E,Poirier L,et al.Size dependent bioaccumulation and ecotoxicity of gold nanoparticles in an endobenthic invertebrate:The tellinid clam Scrobicularia plana[J].Environmental Pollution,2012,168:37-43.
• [12] Mccarrick S,Cunha V,Zapletal O,et al.In vitro and in vivo genotoxicity of oxygenated polycyclic aromatic hydrocarbons[J].Environmental Pollution,2019,246:678-687.
• [13] Dos Santos D S,Alvarez-Puebla R A,Oliveira O N,et al.Controlling the size and shape of gold nanoparticles in fulvic acid colloidal solutions and their optical characterization using SERS[J].Journal of Materials Chemistry,2005,15(29):3045-3049.
• [14] Afshinnia K,Baalousha M.Effect of phosphate buffer on aggregation kinetics of citrate-coated silver nanoparticles induced by monovalent and divalent electrolytes[J].Science of the Total Environment,2017,581:268-276.
• [15] Afshinnia K,Marrone B,Baalousha M.Potential impact of natural organic ligands on the colloidal stability of silver nanoparticles[J].Science of the Total Environment,2018,625:1518-1526.
• [16] Chen C S,Le C,Chiu M H,et al.The impact of nanoplastics on marine dissolved organic matter assembly[J].Science of the Total Environment,2018,634:316-320.
• [17] Neubauer C M,Yang M,Jennings H M.Interparticle potential and sedimentation behavior of cement suspensions:Effects of admixtures[J].Advanced Cement Based Materials,1998,8(1):17-27.
• [18] Viarengo A,Ponzano E,Dondero F,et al.A simple spectrophotometric method for metallothionein evaluation in marine organisms:An application to Mediterranean and Antarctic molluscs[J].Marine Environmental Research,1997,44(1):69-84.
• [19] Moltedo O,Verde C,Capasso A,et al.Zinc transport and metallothionein secretion in the intestinal human cell line Caco-2[J].Journal of Biological Chemistry,2000,275(41):31819-31825.
• [20] 林芃，任宏伟，茹炳根.鱼体内金属硫蛋白与水环境关系的研究[J].北京大学学报(自然科学版),2001,37(6):779-784.Lin P,Ren H W,Ru B G.Study of metallothionein in fishes for Donghu aquatic environment[J].Acta Scientiarum Naturalium Universitatis Pekinensis,2001,37(6):779-784.
• [21] Ward J E,Levinton J S,Shumway S E,et al.Site of particle selection in a bivalve mollusc[J].Nature,1997,390(6656):131-132.
• [22] Yu S,Liu J,Yin Y,et al.Interactions between engineered nanoparticles and dissolved organic matter:A review on mechanisms and environmental effects[J].Journal of Environmental Sciences,2018,63:198-217.
• [23] Nel A,Xia T,Madler L,et al.Toxic potential of materials at the nanolevel[J].Science,2006,311(5761):622-627.
• [24] Pan J F,Wang W X.Comparison of the bioavailability of Cr and Fe bound with natural colloids of different origins and sizes to two marine bivalves[J].Marine Biology,2002,141(5):915-924.
• [25] Pan J F,Wang W X.Influences of dissolved and colloidal organic carbon on the uptake of Ag,Cd,and Cr by the marine mussel Perna viridis[J].Environmental Pollution,2004,129(3):467-477.
• [26] Sandrini J Z,Bianchini A,Trindade G S,et al.Reactive oxygen species generation and expression of DNA repair-related genes after copper exposure in zebrafish (Danio rerio) ZFL cells[J].Aquatic Toxicology,2009,95(4):285-291.
• [27] Kienzler A,Bony S,Devaux A.DNA repair activity in fish and interest in ecotoxicology:A review[J].Aquatic Toxicology,2013,134-135:47-56.
• [28] Wilson J T,Pascoe P L,Parry J M,et al.Evaluation of the comet assay as a method for the detection of DNA damage in the cells of a marine invertebrate,Mytilus edulis L.(Mollusca:Pelecypoda)[J].Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis,1998,399(1):87-95.

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