琚彩霞,徐甲坤,张权,孙谧

• 1:中国水产科学研究院黄海水产研究所
• 2:上海海洋大学食品学院

摘要(Abstract)：

采用化学共沉淀法合成磁性Fe3O4纳米粒子(Magnetic Nanoparticles,MNP),先后用正硅酸乙酯(TEOS)和3-氨丙基三乙氧基硅烷(APTES)对MNP进行表面功能修饰,成功制备了硅烷化磁性纳米粒子(SMNP)和氨基磁性纳米微球(AMNP)。通过透射电镜(TEM)、傅立叶红外变换光谱仪(FTIR)、X-射线衍射仪(XRD)、超导量子干涉仪(SQUID)和热重分析仪(TGA)对合成粒子进行形貌、结构、磁性能和化学组成进行表征。测试结果表明,合成材料平均粒径约为7~10nm,结晶性能良好;3种粒子均具有良好的超顺磁性,MNP、SMNP和AMNP的饱和磁化强度值分别为47.9、33.0和19.5emu/g;氨基含量为5.7%。利用AMNP对牛血清白蛋白(BSA)开展酶的固定化研究,最大吸附量为41mg/g。

关键词(KeyWords)： 磁性纳米粒子;表面功能修饰;BSA固定化

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展计划项目(2011AA090703);; 国家自然科学基金项目(31200642);; 青岛市科技计划项目(12-1-4-12-(2)-jch);; 中国水产科学研究院基本科研业务费(2013A1002)资助

作者(Author): 琚彩霞,徐甲坤,张权,孙谧

DOI: 10.16441/j.cnki.hdxb.2013.11.010

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