陈睿,杜国英,管晓伟,陈念慈,曹敏,茅云翔,王冬梅

• 1:中国海洋大学海洋生命学院
• 2:青岛农业大学海洋科学与工程学院
• 3:海南热带海洋学院水产与生命学院

摘要(Abstract)：

为探究坛紫菜响应失水胁迫的机制，以坛紫菜(Pyropia haitanensis)为研究对象，采用超高效液相色谱-串联四级杆质谱(UPLC-MS/MS)技术分析了坛紫菜叶状体在失水/复水胁迫下的代谢组学变化。在坛紫菜提取物中检测和鉴定到了大量代谢物(已知的有206种)。利用Analyst 1.6.1软件对原始质谱数据进行采集，通过偏最小二乘判别分析法(PLS-DA)分析主成分，寻找差异代谢物。本研究发现坛紫菜失水组与正常对照组的代谢组间共有58种差异代谢物，失水条件下有38种上调，20种下调，主要包括一些氨基酸类及氨基酸衍生物、苯丙素类、甘油磷脂、核苷酸及其衍生物、黄酮类及类黄酮类、还有植物激素；富集到3条代谢通路，分别是氨酰-tRNA生物合成、嘌呤代谢以及半胱氨酸和蛋氨酸代谢通路。具有抗氧化能力的黄酮类物质、渗透保护因子脯氨酸和甜菜碱在叶状体失水时含量均升高，这表明它们在坛紫菜响应失水逆境中起重要的作用。坛紫菜失水组与复水组组间也存在较大代谢物差异，共筛选出50种差异代谢物，复水后有30种上调，20种下调，主要包括儿茶素及其衍生物、甘油磷脂、黄酮类、脂肪酸、萜类、氨基酸衍生物、苯丙素类以及植物激素类；富集到5条代谢通路，包括：半胱氨酸/蛋氨酸代谢、甘氨酸/丝氨酸/苏氨酸的代谢、D-丙氨酸代谢、氨酰-tRNA生物合成和ABC转运通路。坛紫菜复水组与对照组的组间有42种差异代谢物。与对照组相比，复水组有31种代谢物上调和11种下调，主要包括氨基酸类及氨基酸衍生物、儿茶素衍生物、黄酮类、苯丙素类、萜类和脂肪酸等，仅富集到半胱氨酸和蛋氨酸代谢这一条代谢通路。多种代谢物的变化说明坛紫菜中有多种响应胁迫的策略来使其适应生存环境。

关键词(KeyWords)： 坛紫菜;代谢组;失水胁迫;复水;差异代谢物;代谢通路

Abstract:

Keywords:

基金项目(Foundation): 山东省自然科学基金项目(ZR2019MC012)资助~~

作者(Author): 陈睿,杜国英,管晓伟,陈念慈,曹敏,茅云翔,王冬梅

DOI: 10.16441/j.cnki.hdxb.20210173

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