尚孟慧,臧晓南,林娇娇,李瑞,毕莹,徐晓婷

• 1:中国海洋大学海洋生物遗传学与育种教育部重点实验室

摘要(Abstract)：

为了探究藻胆蛋白与高等植物类囊体膜之间的光能传递规律,验证重组藻胆蛋白是否可以与高等植物类囊体膜之间的光能传递,本研究分别提取了重组藻红蛋白、重组藻蓝蛋白以及菠菜和韭菜的类囊体进行混合孵育,然后进行了全波长吸收光谱以及荧光光谱检测。结果显示:混合孵育时间为10～20 min有利于重组藻红蛋白和藻蓝蛋白与类囊体膜结合并进行光能传递,最适混合孵育时间随重组藻胆蛋白和类囊体的种类不同而稍有不同;重组藻红蛋白不能与菠菜或韭菜的类囊体膜直接发生光能传递,但是当体系中添加重组藻蓝蛋白的时候就可以;重组藻蓝蛋白可以与类囊体中的叶绿素a直接进行光能传递,使得叶绿素a的荧光发射峰值显著提升,当藻红蛋白和藻蓝蛋白的比例是1∶2时,光能传递效率较高。本研究为藻胆蛋白在构建新型光合系统中的应用提供实验依据。

关键词(KeyWords)： 藻红蛋白;藻蓝蛋白;重组表达;类囊体膜;光能传递

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31872555)资助~~

作者(Author): 尚孟慧,臧晓南,林娇娇,李瑞,毕莹,徐晓婷

参考文献(References)：

• [1] Zhu B H,Wang G C,Tseng C K.Interaction between phycobilisomes from Porphyridium cruentum and thylakoid membranes from Gymnodinium sp.or spinach[J].Russian Journal of Plant Physiology,2005,52(3):288-293.
• [2] 张学成，程晓杰，施定基.江蓠属藻胆蛋白的研究——Ⅱ.龙须菜藻胆蛋白光谱特性和荧光动力学研究[J].海洋学报(中文版),1999(1):3-5.Zhang X C,Cheng X J,Shi D J.Studies on phycobiliprotein in Gracilaria Ⅱ.Characterization on aborption and emission spera and fluorescent dynamics in intact thallus of Gracilaria lemaneiformis[J].Acta Oceanologica Sinica(Chinese),1999,(1):3-5.
• [3] 冯小亭，臧晓南，张学成，等.藻胆体与类囊体膜之间的能量传递研究进展[J].科技资讯，2015(27):245-251.Feng X T,Zang X N,Zhang X C,et al.Advances in energy transfer between phycobilisomes and thylakoid membrane[J].Science & Technology Information,2015 (27):245-251.
• [4] 仵小南，周百成，曾呈奎.藻胆蛋白和高等植物类囊体之间的能量传递[J].植物学报，1993(9):657-663.Wu X N,Zhou B C,Zeng C K.Light energy transfer between phycobiliproteins and thylakoids of higher plants[J].Journal of Integrative Plant Biology,1993(9):657-663.
• [5] Su X,Fraenkel P G,Bogoard L.Excitation energy transfer from phyococyanin to chlorophyll in an apcA-defective mutant of Sychosystis sp.PCC6803[J].J Biol Chem,1992,267:22944-22950.
• [6] 容寿榆，俞国强，单晓亮，等.蓝藻藻胆体与菠菜光系统Ⅱ颗粒之间的能量传递[J].植物学报，1998(7):3-5.Rong S Y,Yv G Y,Shan X L,et al.Energy transfer from cyanobacterial phycobilisomes to spinach photosystemⅡ partucles[J].Acta Botanica Sinica,1998(7):3-5.
• [7] 陈小全，田坤新，邵辉莹，等.菠菜叶绿素的提取及叶绿素铜钠盐的制备[J].泰山医学院学报，2010(4):259-261.Chen X Q,Tian K X,Shao H Y,et al.Extraction of spinach pigments by improved method and the preparation for sodium copper chlorophyllin[J].Journal of Taishan Medical College,2010(4):259-261.
• [8] 吴平，韩静毅，陈海香.菠菜中叶绿素的提取及紫外光谱研究[J].广州化工，2016(19):144-145.Wu P,Han J Y,Chen H X.Extraction of chlorophyll from spinach and its ultraviolet spectrum[J].Guangzhou Chemical Industry,2016(19):144-145.
• [9] 张秀君，孙钱钱，乔双，等.菠菜叶绿素提取方法的比较研究[J].作物杂志，2011(3):57-60.Zhang X J,Sun Q Q,Qiao S,et al.A comparative study of chlorophyll extraction methods[J].Crops,2011(3):57-60.
• [10] 高岐，赵旭光，谭煜媚.分光光度法测定-超声波提取植物韭菜中叶绿素含量的研究[J].食品工业科技，2007,8:166-167.Gao Q,Zhao X G,Tian Y M.Study on ultrasonic wave extraction and spectrophotometry determination of chlorophyll in plant leek[J].Science and Technology of Food Industry,2007,8:166-167.
• [11] 李文军，蒲洋，牛壮，等.重组别藻蓝蛋白三聚体结构与功能[J].科学通报，2017(16):1699-1713.Li W J,Pu Y,Niu Z,et al.Structural and functional investigation of allophycocyanin trimer[J].Chinese Science Bulletin,2017(16):1699-1713.
• [12] 吴非.具光学活性藻胆蛋白重组合成的研究[D].青岛：中国海洋大学，2016.Wu F.Study on Recombinant Synthesis of Optically Active Phycobiliprotein[D].Qingdao:Ocean University of China,2016.
• [13] 路荣昭，刘斌，李凤平.螺旋藻(Spirulina platensis)藻胆体在解离过程中荧光发射光谱和光能传递的研究[J].生物物理学报，1994,10(3):479-484.Lu R Z,Liu B,Li F P.Studies on the fluorescence emission spectra and energy transfer of phycobilisomes from Spirulina platensis in the course of dissociation[J].Acta Biophysica Sinica,1994,10(3):479-484.
• [14] 俞国强，张辉，容寿榆.藻蓝蛋白与含叶绿素a脂质体之间的能量传递[J].植物学报，1999,41 (1):3-5.Yu G Q,Zhang H,Rong S Y.Energy transfer from cyanobacterial phycocyanins to chlorophyll a-containing liposomes[J].Acta Botanica Sinica,1999,41 (1):3-5.
• [15] 刘冬，李功藩.铜对类囊体膜叶绿素-蛋白质复合物组成和光谱特性的影响[J].武汉植物学研究，1992(1):65-72.Liu D,Li G F.Efferts of copper on The chlorophyllprotein complexes components and some spectral properties of chloroplast thylakoid membranes[J].Journal of Wuhan Botanical Research,1992(1):65-72.
• [16] Mullineaux C W.Phycobilisome-reaction centre interaction in cyanobacteria[J].Photosynthesis Research,2008,95(2-3):175-182.
• [17] Busch A,Nield J,Hippler M.The composition and structure of photosystem I-associated antenna from Cyanidioschyzon merolae[J].Plant J,2010,62(5):886-897.
• [18] Bryant D A,Frigaard N U.Prokaryotic photosynthesis and phototrophy illuminated[J].Trends Microbiol,2006,14(11):488-496.
• [19] Glazer A N.Light harvesting by phycobilisomes[J].Annu Rev Biophys Biophys Chem,1985,14:47-77.
• [20] Glazer A N,Melis A.Photochemical reaction centers:Structure,organization,and function[J].Annual Review of Plant Physiology,1987,38(1):11-45.
• [21] Glazer A N,Hixson C S.Subunit structure and chromophore composition of rhodophytan phycoerythrins.Porphyridium cruentum B-phycoerythrin and b-phycoerythrin[J].J Biol Chem,1977,252(1):32-42.
• [22] Mullineaux C W.Phycobilisome-reaction centre interaction in cyanobacteria[J].Photosynth Res,2008,95(2-3):175-182.
• [23] Tooley A J,Cai Y A,Glazer A N.Biosynthesis of a fluorescent cyanobacterial C-phycocyanin holo-alpha subunit in a heterologous host[J].Proc Natl Acad Sci,2001,98(19):10560-10565.
• [24] Li Y.Studies on energy transfer between phycobiliproteins and thylakoid complex[J].Advanced Materials Research,2012,343-344:748-752.

文章评论(Comment)：