王司玉,隋修国,李正炎

• 1:中国海洋大学环境科学与工程学院
• 2:中国海洋大学海洋环境与生态教育部重点实验室

摘要(Abstract)：

本文采用频数分布法和压力-响应模型法分别推导小清河口营养物基准,其中频数分布法根据河口所有监测站位营养物浓度的下第5个百分点作为基准值;压力-响应模型法选取小清河口三种常见赤潮藻中肋骨条藻(Skeletonema costatum)、赤潮异弯藻(Heterosigma akashiwo)和微型原甲藻(Prorocentrum minimum)为实验对象,建立河口营养物水平与浮游藻生长之间的压力响应关系模型并推导生态基准值。频数分布法得出的小清河口总氮(TN)、总磷(TP)、溶解无机氮(DIN)和活性磷酸盐(SRP)基准值分别为5.18、0.14、3.33和0.015 mg/L。由于小清河口氮污染严重,现场调查得到的TN和DIN均值均远超我国地表水环境质量标准IV类值(1.5 mg/L)和海水水质标准IV类值(0.5 mg/L),因此频数分布法推导的TN和DIN基准值不推荐为小清河口营养物基准值。压力-响应模型法推导的四种营养物基准值分别为0.23、0.059、0.17和0.020 mg/L,该值可作为小清河口营养物基准推荐值,以期为小清河口营养物标准制定和生态风险评估提供科学依据。

关键词(KeyWords)： 河口营养物基准;小清河口;压力-响应模型;频数分布法

Abstract:

Keywords:

基金项目(Foundation): 国家重点研究发展计划项目(2018YFC1407601)资助~~

作者(Author): 王司玉,隋修国,李正炎

参考文献(References)：

• [1] Feng C L,Li H,Yan Z F,et al.Technical study on national mandatory guideline for deriving water quality criteria for the protection of freshwater aquatic organisms in China[J].Journal of Environmental Management,2019,250:1-7.
• [2] US EPA (US Environmental Protection Agency).National Recommended Water Quality Criteria:2002[R].Washington DC:United States Environmental Protection Agency′,Office of Water,Office of Science andTechnology(4304T),2002.
• [3] US EPA.National Recommended Water Quality Criteria[R].Washington DC:United States Environmental Protection Agency′,Office of Water,Office of Science and Technology,2009.
• [4] Anzecc A.Australian and New Zealand Guidelines for Fresh and Marine Water Quality[R].Canberra:Australian and New Zealand Environment and Conservation Council and Agriculture and Resource Management Council of Australia and New Zealand,2000:1-103.
• [5] European Chemicals Bureau.Technical Guidance Document on Risk Assessment-Part II[R].Luxembourg:European Chemicals Bureau,2003.
• [6] CCME A.Protocol for the Derivation of Water Quality Guidelines for the Protection of Aquatic Life[R].Winnipeg,Manitoba:Canadian Council of Ministers of the Environment,2007.
• [7] Van Vlaardingen P L A,Verbruggen E M J.Guidance for the Derivation of Environmental Risk Limits Within the Framework of ‘International and National Environmental Quality Standards for Substances in the Netherlands’(INS).Revision 2007[R].Netherland:National Institute for Public Health and the Environment,2007.
• [8] World Health Organization (WHO).Guidelines for Drinking-Water Quality Incorporation 1st and 2nd Addenda[R].Geneva,Switzerland:World Health Organization,2008.
• [9] 李俊龙，郑丙辉，刘永，等.中国河口富营养化对营养盐负荷的敏感性分类[J].中国科学：地球科学，2015,45(4):455-467.doi:10.1007/s11430-014-5030-1.Li J L,Zheng B H,Liu Y,et al.Classification of estuaries in China based on eutrophication susceptibility to nutrientload[J].Science China:Earth Sciences,2015,45(4):455-467.doi:10.1007/s11430-014-5030-1.
• [10] Rabalais N N.Nitrogen in aquatic ecosystems[J].AMBIO:A Journal of the Human Environment,2002,31(2):102-113.
• [11] Howarth R,Chan F,Conley D J,et al.Coupled biogeochemical cycles:eutrophication and hypoxia in temperate estuaries and coastal marine ecosystems[J].Frontiers in Ecology and the Environment,2011,9(1):18-26.
• [12] US EPA.Nutrient Criteria Technical Guidance Manual:Estuarine and Coastal Marine Waters[R].Washington DC:United States Environmental Protection Agency′,Office of Water,2001.
• [13] 孙丕喜，王波，张朝晖，等.莱州湾海水中营养盐分布与富营养化的关系[J].海洋科学进展，2006(3):329-335.Sun P X,Wang B,Zhang Z H,et al.Relationship between Nutrient distribution and eutrophication in seawater of the Laizhou Bay[J].Advances in Marine Science,2006(3):329-335.
• [14] 张雪.近三十年莱州湾主要污染物时空变化及小清河口水质评价指标体系建立[D].青岛：中国海洋大学，2013.Zhang X.Spatio-Temporal Variability of Principal Pollutants in Laizhou Bay in Nearly 30 Years and Establishment of Evaluation Index System in Water of Xiaoqing River Estuary[D].Qingdao:Ocean University of China,2013.
• [15] 祝雅轩，裴绍峰，张海波，等.莱州湾营养盐和富营养化特征与研究进展[J].海洋地质前沿，2019,35(4):1-9.Zhu Y X,Pei S F,Zhang H B,et al.Characteristics and research progress of Nutrients and eutrophication in Laizhou Bay,China[J].Marine Geology Frontiers,2019,35(4):1-9.
• [16] 刘义豪，杨秀兰，靳洋，等.莱州湾海域营养盐现状及年际变化规律[J].渔业科学进展，2011,32(4):1-5.Liu Y H,Yang X L,Jin Y,et al.Distribution and inter-annual variation of nutrients in Laizhou Bay[J].Progress in Fishery Science,2011,32(4):1-5.
• [17] 国家环保局《水和废水监测分析方法》编委会编.水和废水监测分析方法第3版[M].北京：中国环境科学出版社，1989.National Environment Protection Agency,Editorial Board of Water and Wastewater Monitoring and Analysis Method.Water and Wastewater Monitoring and Analysis Method[M].(3rd Edition).Beijing:China Environmental Science Press,1989.
• [18] 国家环境保护部.GB/T 11894—1989,水质总氮的测定碱性过硫酸钾消解紫外分光光度法[S].北京：中国标准出版社，1989.National Ministry of Environmental Protection.GB/T 11894—1989,Water Quality—Determination of Total Nitrogen—Alkaline Potassium Persulfate Digestion-UV Spectrophotometric Method[S].Beijing:China Standards Press,1989.
• [19] 国家环境保护部.GB/T 11893—1989,水质总磷的测定钼酸铵分光光度法[S].北京：中国标准出版社，1989.National Ministry of Environmental Protection.GB/T 11893—1989,Water Quality—Determination of Total Phosphorus—Ammonium Molybdate Spectrophotometric Method[S].Beijing:China Standards Press,1989.
• [20] 国家环境保护部.GB/T 7479—1987,水质铵的测定纳氏试剂比色法[S].北京：中国标准出版社，1987.National Ministry of Environmental Protection.GB/T 7479—1987,Water Quality—Determination of Ammonium—Nessler’s Reagent Colorimetric Method[S].Beijing:China Standards Press,1987.
• [21] 国家环境保护部.GB/T 7493—1987,水质亚硝酸盐氮的测定分光光度法[S].北京：中国标准出版社，1987.National Ministry of Environmental Protection.GB/T 7493—1987,Water Quality-Determination of Nitrogen(Nitrite)-Spectrophotometric Method[S].Beijing:China Standards Press,1987.
• [22] US EPA (US Environmental Protection Agency).Nutrient Criteria Technical Guidance Manual:Rivers and Streams[R].Washington DC:United States Environmental Protection Agency′,Office of Water,2000.
• [23] US EPA.Nutrient Criteria Technical Guidance Manual:Lakes and Reservoirs[R].Washington DC:United States Environmental Protection Agency′,Office of Water,2000.
• [24] 夏青，陈艳卿，刘宪兵.水质基准与水质标准[M].北京：中国标准出版社，2004:362-365.Xia Q,Chen Y Q,Liu X B.Water Quality Criteria and Water Quality Standards[M].Beijing:Station Pres of China,2004:362-365.
• [25] 霍守亮，马春子，席北斗，等.湖泊营养物基准研究进展[J].环境工程技术学报，2017,7(2):125-133.Huo S L,Ma C Z,Xi B D,et al.Progress in research on lake nutrient criteria[J].Journal of Environmental Engineering Technology,2017,7(2):125-133.
• [26] US EPA.Using Stressor-Response Relationships to Derive numeric Nutrient Criteria[R].Washington DC:United States Environmental Protection Agency′,Office of Water,2010.
• [27] Thomas Heatherly II.Acceptable nutrient concentrations in agriculturally dominant landscapes:A comparison of nutrient criteria approaches for Nebraska rivers and streams[J].Ecological Indicators,2014,45:355-363.
• [28] 王修林，邓宁宁，祝陈坚，等.磷酸盐、硝酸盐组成对海洋赤潮藻生长的影响[J].中国海洋大学学报(自然科学版),2004(3):453-460.Wang X L,Deng N N,Zhu C J,et al.Effect of Nutrients (Phosphate and Nitrate) composition on the growth of HAB Algae[J].Periodical of Ocean University of China,2004(3):453-460.
• [29] 杨福霞.大辽河口营养物基准值的制定方法及其影响因素研究[D].青岛：中国海洋大学，2015.Yang F X.Approaches of Nutrient Criteria Establishment and its Influencing Factors in the Daliaohe Estuary[D].Qingdao:Ocean University of China,2015.
• [30] 梁文懂，米本年邦.绿藻的生长动力学及其模拟研究[J].武汉冶金科技大学学报(自然科学版),1999(2):26-28.Liang W D,Toshikuni Y.Kinetics and simulation of culture of chlorella ellipsoidea[J].Journal of Wuhan Yejin University of Science and Technology (Natural Science Edition),1999(2):26-28.
• [31] 于宁，于建生，吕振波，等.山东海域赤潮灾害特征及预警报管理[J].生态学杂志，2012,31(5):1272-1281.Yu N,Yu J S,Lv Z B,et al.Disaster characteristics of harmful algal bloom and its early warning management in Shandong coastal waters[J].Chinese Journal of Ecology,2012,31(5):1272-1281.
• [32] 林凤翱，卢兴旺，洛昊，等.渤海赤潮的历史、现状及其特点[J].海洋环境科学，2008,27(S2):1-5.Lin F A,Lu X W,Luo H,et al.History,status and characteristics of red tide in Bohai Sea[J].Marine Environmental Science,2008,27(S2):1-5.
• [33] 国家环境保护部.GB 3097—1997,海水水质标准[S].北京：中国标准出版社，1997.National Ministry of Environmental Protection.GB 3097—1997,Sea Water Quality Standard[S].Beijing:China Standards Press,1997.
• [34] 刘皓，高永利，殷克东，等.不同氮磷比对中肋骨条藻和威氏海链藻生长特性的影响[J].热带海洋学报，2010,29(6):92-97.Liu H,Gao Y L,Yin K D,et al.Effects of N and P ratio on the growth of two red tide diatom Skeletonema costatum and Thalassiosira weissflogii[J].Journal of Tropical Oceanography,2010,29(6):92-97.
• [35] Redfield A C.The biological control of chemical factors in the environment[J].American Scientist,1958,46(3):205-221.
• [36] Robert R L Guillard,John H Ryther.Studies of marine planktonic diatoms:I.Cyclotella nana hustedt,and detonula confervacea (cleve) gran[J].Canaian.Journal of Microbiology,1962,8(2):229-239.
• [37] 董正臻，董振芳，丁德文.快速测定藻类生物量的方法探讨[J].海洋科学，2004(11):1-2+5.Dong Z Z,Dong Z F,Ding D W.A method of quick determination of algal biomass[J].Marine Sciences,2004(11):1-2+5.
• [38] 李胜男，王秀娟，周建，等.利用流式细胞仪计数微型浮游生物的方法[J].湖泊科学，2015,27(5):757-766.Li S N,Wang X J,Zhou J,et al.Application of flowcytometry to enumerate small plankton[J].Journal of Lake Sciences,2015,27(5):757-766.
• [39] Mooney C F,Mooney C L,Mooney C Z,et al.Bootstrapping:A Nonparametric Approach to Statistical Inference[M].Washington DC:Sage Publications,1993.
• [40] 范新凤，韩美，王磊，等.小清河入海口近十年水质变化及驱动因素分析[J].环境科学，2020,41(4):1619-1628.Fan X F,Han M,Wang L,et al.Analysis of water quality change and its driving factors of the Xiaoqing River estuary in recent ten years[J].Environmental Science,2020,41(4):1619-1628.
• [41] 崔燕，张龙军，罗先香，等.小清河口水质污染现状及富营养化评价[J].中国海洋大学学报(自然科学版),2013,43(2):60-66.Cui Y,Zhang L J,Luo X X,et al.Study on the water pollution and eutrophication in the Xiaoqing River estuary[J].Periodical of Ocean University of China,2013,43(2):60-66.
• [42] 国家环境保护部.GB 3838—2002,地表水环境质量标准[S].北京：中国标准出版社，2002.National Ministry of Environmental Protection.GB 3838—2002,Environmental Quantity Standards for Surface Water[S].Beijing:China Standards Press,2002.
• [43] 苏静，杨福霞，苏荣国，等.辽河口营养物基准值的制定方法[J].中国海洋大学学报(自然科学版),2016,46(9):78-84.Su J,Yang F X,Su R G,et al.Approach of nutrient criteria establishment and application in the Liaohe estuary[J].Periodical of Ocean University of China,2016,46(9):78-84.
• [44] 胡莹莹，王菊英，张志锋，等.辽河口近岸海域水体营养物推荐基准值的制定方法[J].中国环境科学，2011,31(6):996-1000.Hu Y Y,Wang J Y,Zhang Z F,et al.Approach of nutrient criteria establishment and application in coastal area of Liaohe estuary[J].China Environmental Science,2011,31(6):996-1000.

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