王晓艳,马季,王际英,李宝山,黄炳山,郝甜甜,王成强,宋志东,李培玉,孙永智

• 1:山东省海洋资源与环境研究院山东省海洋生态修复重点实验室
• 2:上海海洋大学水产科学国家级实验教学示范中心

摘要(Abstract)：

为研究高植物蛋白饲料中添加鱼溶浆粉对珍珠龙胆石斑鱼(Epinephelus fuscoguttatus♀×E.lanceolatu■)幼鱼的生长性能、体组成、血清生化指标、非特异性免疫及肠道组织结构的影响。以(23.72±0.09) g的珍珠龙胆石斑鱼幼鱼为研究对象,添加60%白鱼粉为对照组(DF),用30%大豆浓缩蛋白替代饲料中30%的白鱼粉,并添加0、2.5%、5%、7.5%和10%的鱼溶浆粉(分别命名为D0、D2.5、D5、D7.5和D10),配制了6组等氮等能的实验饲料,进行为期8周的养殖实验。实验表明:添加5%以上鱼溶浆粉幼鱼的增重率、特定生长率、肝体比和脏体比与DF组差异不显著(P>0.05);添加鱼溶浆粉组的全鱼粗蛋白均显著高于DF组及D0组(P<0.05),其它体成分及背肌氨基酸组成均无显著差异(P>0.05);添加鱼溶浆粉对血清总蛋白、甘油三酯、胆固醇、低密度脂蛋白胆固醇含量和谷丙转氨酶、谷草转氨酶活性影响显著(P<0.05);添加鱼溶浆粉可显著提高血清总抗氧化能力和总超氧化物歧化酶活性并降低丙二醛含量(P<0.05);添加5%以上鱼溶浆粉,肠道皱襞数目与DF组无显著差异(P>0.05),D0组皱襞高度显著低于其它各组(P<0.05),肌层厚度受不同饲料影响不显著(P>0.05)。研究结果表明,在本实验条件下,高植物蛋白饲料中添加5%以上的鱼溶浆粉能达到与30%的鱼粉相同的饲喂效果。

关键词(KeyWords)： 珍珠龙胆石斑鱼;鱼溶浆粉;高植物蛋白饲料;生长性能;体组成;血清生化指标;非特异性免疫;肠道组织结构

Abstract:

Keywords:

基金项目(Foundation): 烟台市重点研究发展计划项目(2017ZH066,2016ZH068,2018ZHGY066,2020XDRH091);; 山东省重点研究发展项目(2016GSF115005)资助~~

作者(Author): 王晓艳,马季,王际英,李宝山,黄炳山,郝甜甜,王成强,宋志东,李培玉,孙永智

DOI: 10.16441/j.cnki.hdxb.20190282

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