3种鲻科鱼类线粒体COⅠ基因片段序列的比较分析Sequence Analysis of COⅠ Gene Fragment of Three Mugilidae Species
杨天燕,高天翔,孟玮,孙利元
摘要(Abstract):
运用PCR技术,扩增了3种鲻科鱼类梭鱼(Chelon haematocheilus)、棱梭(Chelon carinata)和鲻鱼(Mugil cephalus)线粒体COⅠ基因片段,并比较分析了3种间的序列差异。测序得到510 bp的碱基序列,3种间均未出现碱基的插入与缺失。序列中G平均含量最低,且A+T含量高于G+C含量,与其他鱼类COⅠ基因片段研究结果相一致。在COⅠ基因片段中,梭鱼和棱梭样本各出现2种单倍型,而3个鲻鱼个体均为同一单倍型。计算得出3种鲻科鱼类遗传距离在0.085~0.219之间。以库里玛鲻(Mugil curema)为外群构建的NJ系统发育树显示,梭鱼与棱梭间的遗传关系较近,而与鲻鱼的遗传关系较远,这一结论与形态分类学研究结果一致。
关键词(KeyWords): 梭鱼;棱梭;鲻鱼;COⅠ基因;序列分析
基金项目(Foundation): 国家高技术研究发展计划(2006AA09Z418);; 国家重点基础研究发展计划(2005CB422306)资助
作者(Author): 杨天燕,高天翔,孟玮,孙利元
DOI: 10.16441/j.cnki.hdxb.2007.s2.010
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