唐燎,涂海峰,伍淼,顾书瑞,李锋民

• 1:中国海洋大学近海环境污染控制研究所
• 2:海洋环境与生态教育部重点实验室
• 3:中国海洋大学环境科学与工程学院

摘要(Abstract)：

为揭示环境激素邻苯二甲酸二丁酯(Dibutyl phthalate,DBP)与邻苯二甲酸二乙酯(Diethyl phthalate,DEP)对杜氏盐藻的抑制机理,研究了DBP与DEP对杜氏盐藻(Dunaliella tertiolecta)生长情况、β-胡萝卜素含量、超氧化物歧化酶(superoxide dismutase,SOD)活性、丙二醛(Malonaldehyde,MDA)含量和亚显微结构的影响。结果表明,DBP与DEP对藻细胞生长均有抑制作用,但二者作用机制不同:在DBP作用下藻体SOD活性持续增强,细胞膜结构损伤,MDA含量显著增加,表明DBP可能通过作用藻体的膜结构,引起细胞氧化损伤,最终导致细胞死亡。DEP使得藻体SOD活性先增强后减弱,β-胡萝卜素显著增加,亚显微结构中膜结构未发现明显损伤,但淀粉粒大量增加,线粒体畸形,藻体可能通过合成β-胡萝卜素和淀粉粒抵御氧化胁迫,但大量的物质合成可能导致细胞代谢紊乱,加速藻细胞衰亡。本研究为揭示邻苯二甲酸酯类环境激素的环境行为提供了科学依据。

关键词(KeyWords)： DBP;DEP;杜氏盐藻;抑制机制

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划项目(2015CB453301);; 国家基金委-山东省联合基金项目(U1606404);; 国家自然科学基金项目(51378480)资助~~

作者(Author): 唐燎,涂海峰,伍淼,顾书瑞,李锋民

DOI: 10.16441/j.cnki.hdxb.20170159

参考文献(References)：

• [1]Earls A O,Axford I P,Braybrook J H.Gas chromatography-mass spectrometry determination of the migration of phthalate plasticisers from polyvinyl chloride toys and childcare articles[J].Journal of Chromatography A,2003,983(1-2):237-246.
• [2]Staples C A,Adams W J,Parkerton T F,et al.Aquatic toxicity of eighteen phthalate esters[J].Environmental Toxicology&Chemistry,1997,16(5):875-891.
• [3]中华人民共和国卫生部.中华人民共和国国家标准生活饮用水卫生标准[J].城镇供水,2007(4):27-31.Ministry of Health of the People’s Republic of China.The standard for drinking water quality peaplers republic of China[J].City and Town Water Supply,2007(4):27-31.
• [4]Liu X,Shi J,Bo T,et al.Occurrence of phthalic acid esters in source waters:A nationwide survey in China during the period of2009-2012[J].Environmental Pollution,2013,(184C):262-270.
• [5]Daiem M M A,Rivera-Utrilla J,Ocampo-Pérez R,et al.Environmental impact of phthalic acid esters and their removal from water and sediments by different technologies-A review[J].Journal of Environmental Management,2012,109(2012):164-178.
• [6]Wang P,Wang S L,Fan C Q.Atmospheric distribution of particulate-and gas-phase phthalic esters(PAEs)in a Metropolitan City,Nanjing,East China[J].Chemosphere,2008,72(10):1567-1572.
• [7]Heudorf U,Merschsundermann V,Angerer J.Phthalates:Toxicology and exposure[J].International Journal of Hygiene&Environmental Health,2007,210(5):623-634.
• [8]Latini G.Monitoring phthalate exposure in humans[J].Clinica Chimica Acta,2005,361(1-2):20-29.
• [9]Pant N,Kumar G,Upadhyay A D,et al.Reproductive toxicity of lead,cadmium,and phthalate exposure in men[J].Environmental Science and Pollution Research,2014,21(18):11066-11074.
• [10]Foster P M,Cattley R C,Mylchreest E.Effects of di-n-butyl phthalate(DBP)on male reproductive development in the rat:Implications for human risk assessment[J].Food&Chemical Toxicology,2000,38(1Suppl):S97.
• [11]Takeuchi S,Iida M,Kobayashi S,et al.Differential effects of phthalate esters on transcriptional activities via human estrogen receptors alpha and beta,and androgen receptor[J].Toxicology,2005,210(3):223-233.
• [12]Yan B,Guo J,Liu X,et al.Oxidative stress mediates dibutyl phthalate induced anxiety-like behavior in Kunming mice[J].Environmental Toxicology&Pharmacology,2016,45:45-51.
• [13]Agus H H,Sümer S,Erko9F.Toxicity and molecular effects of di-n-butyl phthalate(DBP)on CYP1A,SOD,and GPx in Cyprinus carpio(common carp)[J].Environmental Monitoring&Assessment,2015,187(7):423.
• [14]Xu H,Dong X,Zhang Z,et al.Assessment of immunotoxicity of dibutyl phthalate using live zebrafish embryos[J].Fish&Shellfish Immunology,2015,45(2):286-292.
• [15]Gao M,Dong Y,Zhang Z,et al.Growth and antioxidant defense responses of wheat seedlings to di-n-butyl phthalate and di(2-ethylhexyl)phthalate stress[J].Chemosphere,2017,172:418-428.
• [16]Dueck T A,Van Dijk C J,David F,et al.Chronic effects of vapour phase di-n-butyl phthalate(DBP)on six plant species[J].Chemosphere,2003,53(8):911-920.
• [17]Howard P H,Printup H T.Handbook of Environmental Degradation Rates[M].Heather Taub.Boca Raton:CRC Press,1991.
• [18]Weyers A,Sokull-Klüttgen B,Baraibar-Fentanes J,et al.Acute toxicity data:A comprehensive comparison of results of fish,daphnia,and algae tests with new substances notified in the European Union[J].Environmental Toxicology&Chemistry,2000,19(7):1931-1933.
• [19]邱海源,王宪.邻苯二甲酸酯对海洋藻类的致毒效应[J].厦门大学学报(自然版),2005,44(2):294-296.Qiu Haiyuan,Wang Xian.Toxic effects of phthalic esters on algae[J].Journal of Xiamen University,2005,44(2):294-296.
• [20]迟杰,董林林,李金娟.铜绿微囊藻对DBP和DEHP的富集和降解特性[J].天津大学学报(自然科学与工程技术版),2006,39(12):1395-1398.Chi Jie,Dong Linlin,Li Jinjuan.Characteristics of accumulation and biodegradation of DBP and DEHP in Microcystis aeruginosa[J].Journal of Tianjin University(Science and Technology),2006,39(12):1395-1398.
• [21]刘春晓,王平,李海燕,等.DBP对铜绿微囊藻生长和抗氧化酶的影响[J].环境科学与技术,2015,38(2):7-12.Liu Chunxiao,Wang Ping,Li Haiyan,et al.Effects of dibutyl phthalate on growth and enzymatic antioxidants of Microcytis aeruginosa[J].Environmental Science&Technology,2015,38(2):7-12.
• [22]黄博珠,何瑞,孙凯峰,等.邻苯二甲酸二丁酯对海洋微藻生长的影响[J].生态毒理学报,2016,11(2):292-299.Huang B Z,He R,Sun K F,et al.Effects of dibutyl phthalate(DBP)on the growth of marine microalgae[J].Asian Journal of Ecotoxicology,2016,11(2):292-299.
• [23]Michael A Borowitzka,Qiang H.18.Dunaliella:Biology,production,and markets[M]//.A Richmond.Handbook of Microalgal Culture:Applied Phycology and Biotech-nology,Second Edition.Hoboken:John Wiley and Sons,Ltd,2013:359-368.
• [24]Belghith T,Athmouni K,Bellassoued K.Physiological and biochemical response of Dunaliella salina to cadmium pollution[J].Journal of Applied Phycology,2016,28(2):1-9.
• [25]Morlon H,Fortin C,Floriani M,et al.Toxicity of selenite in the unicellular green algaChlamydomonas reinhardtii:Comparison between effects at the population and sub-cellular level[J].Aquatic Toxicology,2005,73(1):65-78.
• [26]Jay,A.E.Toxic effects of organic solvents on the growth of Chlorella pyrenoidosaand Selenastrum capricornutum[J].Bulletin of Environmental Contamination and Toxicology,1996,57(2),191-198.
• [27]Borowitzka L J,Borowitzka M A,Moulton T P.The mass culture of Dunaliella salina for fine chemicals:From laboratory to pilot plant[J].Hydrobiologia,1984,116-117(1):115-121.
• [28]Baker C J,Orlandi E W.Active oxygen in plant pathogenesis[J].Annual Review of Phytopathology,1995,33(1):299-321.
• [29]Dong J,Wu F,Zhang G.Influence of cadmium on antioxidant capacity and four microelement concentrations in tomato seedlings(Lycopersicon esculentum)[J].Chemosphere,2006,64(10):1659-1666.
• [30]Benamotz A,Shaish A,Avron M.Mode of action of the massively accumulatedβ-Carotene of Dunaliella bardawil in protecting the algaagainst damage by excess irradiation[J].Plant Physiology,1989,91(3):1040-1043.
• [31]Carranco Jáuregui M E,Calvo C M L,Romo F P.Carotenoids and their antioxidant function:A review[J].Archivos Latinoamericanos De Nutrición,2011,61(3):233-241.
• [32]White A L,Jahnke L S.Contrasting effects of UV-A and UV-B on photosynthesis and photoprotection of beta-carotene in two Dunaliellaspp.[J].Plant&Cell Physiology,2002,43(8):877-884.
• [33]张学成,孟振,时艳侠,等.光照、温度和营养盐对三株盐生杜氏藻生长和色素积累的影响[J].中国海洋大学学报(自然科学版),2006,36(5):754-762.Zhang Xuecheng,Meng Zhen,Shi Yanxia,et al.The Effect of Light,temperature and nutrition on growth and pigment accumulation of three strains of Dunaliella salina[J].Periodical of Ocean University of China,2006,36(5):754-762.
• [34]Lamers P P,Janssen M,De Vos R C,et al.Carotenoid and fatty acid metabolism in nitrogen-starved Dunaliella salina,a unicellular green microalga[J].Journal of Biotechnology,2012,162(1):21-27.
• [35]Sushanta Kumar S,Siobhan M,Patrick M.Effect of macro-and micro-nutrient limitation on superoxide dismutase activities and carotenoid levels in microalgaDunaliella salina CCAP 19/18[J].Bioresource Technology,2013,147(8):23-28.
• [36]Tukaj Z,Bas'cik-Remisiewicz A,Skowroński T,et al.Cadmium effect on the growth,photosynthesis,ultrastructure and phytochelatin content of green microalgaScenedesmus armatus:A study at low and elevated CO2concentration[J].Environmental&Experimental Botany,2007,60(3):291-299.
• [37]Liu N,Wen F,Li F,et al.Inhibitory mechanism of phthalate esters on Karenia brevis[J].Chemosphere,2016,155:498-508.
• [38]Li F M,Wu M,Yao Y,et al.Inhibitory effects and oxidative target site of dibutyl phthalate on Karenia brevis[J].Chemosphere,2015,132:32-39.
• [39]Liu W,Ming Y,Li P,et al.Inhibitory effects of hypo-osmotic stress on extracellular carbonic anhydrase and photosynthetic efficiency of green algaDunaliella salina possibly through reactive oxygen species formation[J].Plant Physiology&Biochemistry,2012,54:43-48.

文章评论(Comment)：