孟范平,何东海,朱小山,马冬冬,杨正先

• 1:中国海洋大学环境科学与工程学院
• 2:中国海洋大学环境科学与工程学院
• 3:中国海洋大学环境科学与工程学院
• 4:中国海洋大学环境科学与工程学院

摘要(Abstract)：

酶的固定化是酶传感器制备过程中重要的1个环节。研究采用直接共价法固定鲅鱼乙酰胆碱酯酶(AChE)。制备方法为:0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h。所制备的固定化酶活力回收率较高(96%),对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%。这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备。

关键词(KeyWords)： 乙酰胆碱酯酶(AChE);鲅鱼(Scomberomorusniphonius);固定化;Sepharose4B

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展计划项目(2001AA635130)资助

作者(Author): 孟范平,何东海,朱小山,马冬冬,杨正先

DOI: 10.16441/j.cnki.hdxb.2005.06.038

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