高保全,刘萍,李健,王清印

• 1:中国海洋大学
• 2:中国水产科学研究院黄海水产研究所

摘要(Abstract)：

准确可靠的遗传力是育种实践的前提,本研究采用不平衡巢式设计和室内定向交尾技术完成亲蟹交配,交配模式为1个雄性三疣梭子蟹配3个雌性三疣梭子蟹,建立100组上述交配组合;经过交尾、越冬,次年春天成功构建了37个三疣梭子蟹全同胞家系,其中13个半同胞家系;80和120日龄时每个家系随机选取30个左右个体,测量其全甲宽、甲长、体高3个生长性状。通过SPSS 11.5一般线性模型(GL.M),计算表型变量的方差组分,评估全甲宽、甲长、体高等重要经济性状的遗传力。结果表明,三疣梭子蟹80日龄3项生长性状总平均值分别为(77.99±11.42)、(35.90±5.84)、(20.13±7.77)mm,80日龄遗传力估计值为0.51~0.69;120日龄3项生长性状总平均值分别为(122.73±12.80)、55.(13±5.39)、(29.99±3.34)mm,120日龄遗传力估计值在0.27~0.70之间。通过t检验发现只有基于全同胞方差组分估计的遗传力达到极显著水平,而基于母系半同胞、父系半同胞方差组分估计的遗传力均未达到显著水平。因此三疣梭子蟹2个发育阶段狭义遗传力的无偏估计值为全同胞方差组分估计的遗传力,估计值分别为0.53~0.60和0.45~0.50。研究结果表明三疣梭子蟹3项重要经济性状属于高度遗传力,说明对其选择具有较大的潜力。

关键词(KeyWords)： 三疣梭子蟹;经济性状;遗传力

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展计划项目(2012AA10A409);; 山东省自然科学基金项目(ZR2013CQ046)资助~~

作者(Author): 高保全,刘萍,李健,王清印

DOI: 10.16441/j.cnki.hdxb.20150246

参考文献(References)：

• [1]吴常文,虞顺成,吕永林.梭子蟹渔业技术[M].上海:上海科学出版社,1996,28-31.Wu C W,Yu S C,Lv Y L.Fisheries Technology of Portunus trituberculatus[M].Shanghai:Shanghai Science Express,1996:28-31.
• [2]农业部渔业局.2006年中国渔业统计年鉴[M].北京:中国农业出版社,2007,54.Fishery Bureau,Ministry of Agriculture,China.China Fisheries Yearbook 2006[M].Beijing:Chinese Agriculture Express,2007:54.
• [3]农业部渔业局.2010年中国渔业统计年鉴[M].北京:中国农业出版社,2011,57.Fishery Bureau,Ministry of Agriculture,China.China Fisheries Yearbook 2010[M].Beijing:Chinese Agriculture Express,2011:57.
• [4]孙颖民,宋志乐,严瑞深.三疣梭子蟹生长的初步研究[J].生态学报,1984,4(1):57-64.Sun Y M,S Z L,Yan R S.Preliminary study on the growth of Portunus trituberculatus[J].Acta Ecologica Sinica,1984,4(1):57-64.
• [5]孙颖民,闫愚,孙进杰.三疣梭子蟹的幼体发育[J].水产学报,1984,8(3):219-226.Sun Y M,Yan Y,Sun J J.The larval development of Portunus trituberculatus[J].Journal of Fisheries of China,1984,8(3):219-226.
• [6]李太武.三疣梭子蟹精子的发生及超微结构的研究[J].动物学报,1995,41(1):41-47.Li T W.On spermatogenesis and sperm ultrastructure of blue crab Portunustriuberbuculatus(Crustacea,Decapoda)[J].Acta Zoologica Sinica,1995,41(1):41-47.
• [7]李太武.三疣梭子蟹肝脏的结构研究[J].海洋与湖沼,1996,27(5):471-477.Li T W.Studies on structure of hepatopancreas in Portunus triuberbuculatus[J].Oceanologia Et Limnologia Sinica,1996,27(5):471-477.
• [8]余红卫,朱东发,韩宝芹.三疣梭子蟹不同组织同工酶的分析[J].动物学杂志,2005,40(1):84-87.Yu HW,Zhu DF,Han BQ.The Isozymes in Different Tissues of Portunus triuberbuculatus[J].Chinese Journal of Zoology,2005,40(1):84-87.
• [9]宋海棠,丁耀平,许源剑.浙江近海三疣梭子蟹洄游分布和群体组成特征[J].海洋通报,1989,8(1):66-74.Song H T,Ding Y P,Xu Y J.Migration distribution and Population composition of Portunus trituberculatus in the Zhejiang offshore[J].Marine Science Bulletin,1989,8(1):66-74.
• [10]朱冬发,王春琳,李志强,等.三疣梭子蟹核型分析[J].水产学报,2005,29(5):649-653Zhu D F,Wang C L,Li Z Q,et al.Karyotype analysis on Portunus trituberculatus[J].Journal of Fisheries of China,2005,29(5):649-653.
• [11]高保全,刘萍,李健,等.三疣梭子蟹四个野生群体形态差异分析[J].中国水产科学,2007,14(2):215-220.Gao B Q,Liu P,Li J,et al.Analysis of morphological variations among four wild populations of Portunus trituberculatus[J].Journal of Fishery Sciences of China,2007,14(2):215-220.
• [12]李晓萍,刘萍,李健,等.应用SSR分析5个三疣梭子蟹野生群体的遗传多样性[J].中国水产科学,2011,18(6):1327-1334.Li X P,Liu P,Li J,et al.Genetic diversity among five wild populations of Portunus trituberculatus[J].Journal of Fishery Sciences of China,2011,18(6):1327-1334.
• [13]樊祥国,高保全,刘萍,等.三疣梭子蟹4个野生群体遗传差异的同工酶分析[J].渔业科学进展,2009,30(4):84-89.Fan X G,Gao B Q,Liu P,et al.Genetic variation analysis of four wild populations of Portunus trituberculatus by isozyme[J].Progress in Fishery Sciences,2009,30(4):84-89.
• [14]戴艳菊,刘萍,高保全,等.三疣梭子蟹4个野生群体四个野生群体线粒体16SrRNA和COⅠ片断的比较分析[J].中国海洋大学学报(自然科学版),2010:40(3):54-60.Dai Y J,Liu P,Gao B Q,et al.Sequence analysis of mitochondrial 16SrRNA and COI gene fragments of four wild populations of Portunus trituberculatus[J].Periodical of Ocean University of China,2010,40(3):54-60.
• [15]高保全,刘萍,李健,等.三疣梭子蟹4个地理群体肥满度研究[J].中国海洋大学学报(自然科学版),2012,42(增刊):51-53.Gao B Q,Liu P,Li J,et al.A preliminary study on relative fatness of Portunus trituberculatus[J].Periodical of Ocean University of China,2012,42(sup):51-53.
• [16]高保全,刘萍,李健,等.三疣梭子蟹形态性状对体重影响的效果分析[J].海洋水产研究,2008,29(1):44-50.Gao B Q,Liu P,Li J,et al.The relationship between morphometric characters and body weight of Portunus trituberculatus[J].Marine Fisheries Research,2008,29(1):44-50.
• [17]刘磊,李健,高保全,等.三疣梭子蟹不同日龄生长性状相关性及对体重的影响[J].水产学报,2009,33(6):964-971.Liu L,Li J,Gao B Q,et al.Correlation of growth of Portunus trituberculatus at the different ages and its impact on body weight[J].Journal of Fisheries of China,2009,33(6):965-972.
• [18]王春琳,吴丹华,崔朝霞,等.三疣梭子蟹选育群F2S表型性状对体质量影响的效果分析[J].海洋科学,2013,37(3):100-108.Wang C L,Wu D H,Cui Z X,et al.The effects of morphometric traits on body weight in F2Population of Swimming crab Portunus trituberculatus[J].Marine Sciences,2013,37(3):100-108.
• [19]Grenshaw J W,Heffernan P B,Walker R L.Heritability of growth rate in the southern Bay scallop,Argopecten irradians concentricus[J].Journal of Shellfish Research,1991,10:55-63.
• [20]Wada K T.Genetic selection for shell traits in Japanese pear oyster,Pinctada fucata martensi[J].Aquaculture,1986,57:171-176.
• [21]Langdon C J,Jacobson D P,Evans F,et al..The molluscan broodstock program improving Pacific oyster broodstock through genetic selection[J].Journal of Shellfish Research,2000,19(1):616.
• [22]Davis C.Estimation of narrow-sense heritability for larval and juvenile growth traits in selected and unselected sub-lines of eastern oysters,Crassostrea virginica[J].Journal of Shellfish Research,2000,19(1):613.
• [23]Aulstad D G,Gjedrem T,Skjervold.Genetic and environmental sources of variation in length and weight of rainbow trout(S.gairdneri)[J].Fish Res Board Can,1972,29:237-341.
• [24]Fishback A G,Danzmann R G,Ferguson M M,et al.Estimates of genetic parameters and genotype by environment interactions for growth traits of rainbow trout(Oncorhynchus mykiss)as inferred using molecular pedigrees[J].Aquaculture,2002,206:137-150.
• [25]Gall G A E.Genetics of reproduction in domesticated rainbow trout[J].J Fish Sci,1975,40:19-28.
• [26]Tave D,Smitherman R O.Predicted response to selection for early growth in Tilapia nilotica[J].Trans Am Fish Soc,1980,109:439-455.
• [27]Quinton C D,McMillan I,Glebe B D.Development of an Atlantic salmon(Salmo salar)genetic improvement program:Genetic parameter of harvest body weight and carcass quality traits estimated with animal models[J].Aquaculture,2005,30:211-217.
• [28]Gunnes K,Gjedrem T.Selection experiments with salmonⅣGrowth of Atlantic salmon during two years in the sea[J].Aquaculture,1978,5:19-23.
• [29]王照旗,韩学凯,白志毅,等.三角帆蚌紫色选育系1龄阶段内壳色及生长性状的遗传参数估计[J].水产学报,2014,38(5):644-650.Wang Z Q,Han X K,Bai Z Y,et al.Estimates of genetic parameters for inner shell color and growth straits during one year old stage in the purple strain of Hyriopsis cumingii using microsatellite based parentage assignment[J].Journal of Fisheries of China,2014,38(5):644-650.
• [30]王俊杰,丁君,常亚青,等.虾夷扇贝家系早中期生长性状比较与遗传参数估计[J].水产学报,2014,38(9):1318-1325.Wang J J,Ding J,Chang Y Q,et al.The growth traits comparison and genetic parameter evaluation of Patinopecten yessoensis in early and mid-stage[J].Journal of Fisheries of China,2014,38(9):1318-1325.
• [31]黎火金,刘宝锁,罗会,等.合浦珠母贝幼贝生长性状的遗传参数估计[J].中国水产科学,2013,20(6):1182-1187.Li H J,Liu B S,Luo H,et al.Estimates of genetic parameters for growth traits in juvenile pearl oyster Pinctada fucata[J].Journal of Fishery Sciences of China,2013,20(6):1182-1187.
• [32]蒋湘,刘建勇,赖志服.九孔鲍(Haliotis diversicolor supertexta)耐低盐与生长性状的遗传参数评估[J].海洋与湖沼,2014,45(3):542-547.Jiang X,Liu J Y,Lai Z F.Estimation of genetic parameter for low salinity tolerance and growth of Haliotis Diversicolor Supertexta[J].Oceanologia Et Limnologia Sinica,2014,45(3):542-547.
• [33]栾生,孙慧玲,孔杰.剌参耳状幼体体长遗传力的估计[J].中国水产科学,2006,13(3):378-383.Luan S,Sun H L,Kong J.Heritability of auricularia larval body length for sea cucumber Apostichopus jeponicus Selenka[J].Journal of Fishery Sciences of China,2006,13(3):378-383.
• [34]徐如卫,钱昭英,刘小林,等.凡纳滨对虾生长性状遗传参数的估计[J].水产学报,2013,37(5):672-678.Xu R W,Qian Z Y,Liu X L,et al.Genetic parameter estimation for growth traits of Pacific white shrimp(Litopenaeus vannamei)[J].Journal of Fisheries of China,2013,37(5):672-678.
• [35]田燚,孔杰,栾生,等.中国对虾生长性状遗传参数的估计[J].海洋水产研究,2008,29(3):1-6.Tian Y,Kong J,Luan S,et al.Estimation of genetic parameters for growth traits of Chinese shrimp Fenneropenaeus chinensis[J].Marine Fisheries Research,2008,29(3):1-6.
• [36]黄付友,何玉英,李健,等.“黄海1号”中国对虾体长遗传力的估计[J].中国海洋大学学报(自然科学版),2008,38(2):269-274.Huang F Y,He Y Y,Li J,et al.Estimates for the Heritability of Body Length in Shrimp Fenneropenaeus chinensis Named“Huanghai No.1”[J].Periodical of Ocean University of China,2008,38(2):269-274.
• [37]田永胜,陈红林,齐文山,等.牙鲆选育F3代家系的建立及遗传效应分析[J].海洋学报,2015,37(4):40-51.Tian Y S,Chen S L,Qi W S,et al.Establishment and estimated genetic effects of Paralichthys olivaceus F3families[J].Acta Oceanologica Sinica,2015,37(4):40-51.
• [38]刘小林.常亚青,相建海,等.虾夷马粪海胆早期生长发育的遗传力估计[J].中国水产科学,2003,10(3):206-211.Liu X L,Chang Y Q,Xiang J H,et al.Heritability of juvenile growth for the sea urchins Strongylocentrotus intermedius[J].Journal of Fishery Sciences of China,2003,10(3):206-211.
• [39]高保全,刘萍,李健,等.三疣梭子蟹体重遗传力的估计[J].海洋与湖沼,2010,41(3):1-5.Gao B Q,Liu P,Li J,et al.Heritability of body weight in Portunus trituberculatus[J].Oceanologia Et Limnologia Sinica,2010,41(3):1-5.
• [40]盛志廉,陈瑶生.数量遗传学[M].北京:科学出版社,2001:60-74.Shen Z L,Chen Y S.Quantitative Genetics[M].Beinjing:Science Express,2001:60-74.
• [41]Benzie J A H,Kenway M,Trott L.Estimates for the heritability of size in juvenile Penaeus monodon prawns from half-sib matings[J].Aquaculture,1997,152:49-53.
• [42]Argue B J,Arce S M,Lotz J M,et al.Selective breeding of Pacific white shrimp(Litopenaeus vannamei)for growth and resistance to Taura Syndrome Virus[J].Aquaculture,2002,204:447-460.

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