李萌,刘如玲,陈进进,彭秋瑜,刘健,佘宗莲

• 1:中国海洋大学海洋环境与生态教育部重点实验室
• 2:青岛海泊河污水处理厂

摘要(Abstract)：

本研究采用复合序批式生物膜反应器(HSBBR)处理高盐废水，考察了进水pH对反应器性能和微生物群落结构的影响。进水pH值由7.8降至6.0和5.0以及由5.0恢复到7.8,对污染物去除效能和稳定性影响较小，COD、NH~+_4-N和总无机氮的平均去除率保持在96%、99%和91%以上。进水pH改变对NO~-_2-N积累产生明显影响，pH为7.8条件下周期内NO~-_2-N最大积累量为7.07～8.15 mg·L~(-1),明显高于pH值为6.0和5.0时的值(4.81和2.23 mg·L~(-1))。进水pH由5.0提升至7.8悬浮污泥和生物膜的微生物多样性均降低。进水pH为5.0时，反应器内相对丰度前三位的菌属为反硝化菌Defluviicoccus(14.7%～27.3%)和Candidatus＿Competibacter(22.7%～26.0%)及聚磷菌Candidatus＿Accumulibacter(11.8%～17.8%);pH提升至7.8时，丰度为前三位的菌属是Candidatus＿Accumulibacter(7.6%～40.5%)、Candidatus＿Competibacter(21.3%～26.2%)和unidentified＿Spirochaetaceae(2.5%～8.7%)。进水pH从5.0升高至7.8,氨氧化菌属Nitrosomonas的相对丰度由3.3%～3.8%下降到1.2%～1.3%,反硝化菌属总丰度从43.5%～52.6%下降到24.9%～33.1%;表明低pH条件下较高丰度氨氧化菌和反硝化菌的共同作用为同步短程硝化反硝化脱氮提供了保障。

关键词(KeyWords)： 高盐;HSBBR;pH;同步短程硝化反硝化;微生物群落

Abstract:

Keywords:

基金项目(Foundation): 中央高校基本科研业务费专项项目(201964003)资助~~

作者(Author): 李萌,刘如玲,陈进进,彭秋瑜,刘健,佘宗莲

DOI: 10.16441/j.cnki.hdxb.20220134

参考文献(References)：

• [1] Bassin J P,Kleerebezem R,Muyzer G,et al.Effect of different salt adaptation strategies on the microbial diversity,activity,and settling of nitrifying sludge in sequencing batch reactors[J].Applied Microbiology and Biotechnology,2012,93(3):1281-1294.
• [2] Castillo-Carvajal L C,Sanz-Martín J L,Barragán-Huerta B E.Biodegradation of organic pollutants in saline wastewater by halophilic microorganisms:A review[J].Environmental Science and Pollution Research,2014,21(16):9578-9588.
• [3] Huang W Y,She Z L,Gao M C,et al.Effect of anaerobic/aerobic duration on nitrogen removal and microbial community in a simultaneous partial nitrification and denitrification system under low salinity[J].Science of the Total Environment,2019,651:859-870.
• [4] She Z L,Zhao L T,Zhang X L,et al.Partial nitrification and denitrification in a sequencing batch reactor treating high-salinity wastewater[J].Chemical Engineering Journal,2016,288:207-215.
• [5] Wang Z,Luo G,Li J,et al.Response of performance and ammonia oxidizing bacteria community to high salinity stress in membrane bioreactor with elevated ammonia loading[J].Bioresource Technology,2016,216:714-721.
• [6] Adham S,Hussain A,Minier-Matar J,et al.Membrane applications and opportunities for water management in the oil & gas industry[J].Desalination,2018,440:2-17.
• [7] Ahmad N N R,Ang W L,Leo C P,et al.Current advances in membrane technologies for saline wastewater treatment:A comprehensive review[J].Desalination,2021,517:115170.
• [8] Zheng S,Sun J,Han H.Effect of dissolved oxygen changes on activated sludge fungal bulking during lab-scale treatment of acidic industrial wastewater[J].Environmental Science & Technology,2011,45(20):8928-8934.
• [9] Jaramillo F,Orchard M,Muňoz C,et al.Advanced strategies to improve nitrification process in sequencing batch reactors - A review[J].Journal of Environmental Management,2018,218:154-164.
• [10] Huesemann M H,Skillman A D,Crecelius E A.The inhibition of marine nitrification by ocean disposal of carbon dioxide[J].Marine Pollution Bulletin,2002,44(2):142-148.
• [11] Yin Z,Santos C E D D,Vilaplana J G,et al.Importance of the combined effects of dissolved oxygen and pH on optimization of nitrogen removal in anammox-enriched granular sludge[J].Process Biochemistry,2016,51(9):1274-1282.
• [12] Jiang Y,Yang K,Shang Y,et al.Response and recovery of aerobic granular sludge to pH shock for simultaneous removal of aniline and nitrogen[J].Chemosphere,2019,221:366-374.
• [13] Zhou H,Wang G C,Wu M H,et al.Phenol removal performance and microbial community shift during pH shock in a moving bed biofilm reactor (MBBR)[J].Journal of Hazardous Materials,2018,351:71-79.
• [14] Cai Y H,Ben T,Zaidi A A,et al.Nitrogen removal augmentation of ship sewage by an innovative aerobic-anaerobic micro-sludge MBR technology[J].Process Biochemistry,2019,82:123-134.
• [15] APHA.Standard Methods for the Examination of Water and Wastewater[M].[s.l.]:American Public Health Association(APHA),2005.
• [16] Yue X,Yu G P,Liu Z H,et al.Fast start-up of the CANON process with a SABF and the effects of pH and temperature on nitrogen removal and microbial activity[J].Bioresource Technology,2018,254:157-165.
• [17] Cai Y,Ben T,Zaidi A A,et al.Effect of pH on pollutants removal of ship sewage treatment in an innovative aerobic-anaerobic micro-sludge MBR system[J].Water,Air,& Soil Pollution,2019,230(7):163.
• [18] Zhu G,Peng Y,Li B,et al.Biological removal of nitrogen from wastewater[M].Reviews of Environmental Contamination and Toxicology,New York:Springer New York,2008:159-195.
• [19] Qian W T,Ma B,Li X Y,et al.Long-term effect of pH on denitrification:High pH benefits achieving partial-denitrification[J].Bioresource Technology,2019,278:444-449.
• [20] He S L,Niu Q G,Li Y Y,et al.Factors associated with the diversification of the microbial communities within different natural and artificial saline environments[J].Ecological Engineering,2015,83:476-484.
• [21] Li T,Guo Z X,She Z L,et al.Comparison of the effects of salinity on microbial community structures and functions in sequencing batch reactors with and without carriers[J].Bioprocess and Biosystems Engineering,2020,43(12):2175-2188.
• [22] He Q,Wang H,Chen L,et al.Robustness of an aerobic granular sludge sequencing batch reactor for low strength and salinity wastewater treatment at ambient to winter temperatures[J].Journal of Hazardous Materials,2020,384:121454.
• [23] Yan P,Wang J,Chen Y P,et al.Investigation of microbial community structure in an advanced activated sludge side-stream reactor process with alkaline treatment[J].International Biodeterioration & Biodegradation,2015,104:356-362.
• [24] Lee S H,Park J H,Kang H J,et al.Distribution and abundance of Spirochaetes in full-scale anaerobic digesters[J].Bioresource Technology,2013,145:25-32.
• [25] Zhang C S,Zhang S Q,Zhang L Q,et al.Effects of constant pH and unsteady pH at different free ammonia concentrations on shortcut nitrification for landfill leachate treatment[J].Applied Microbiology and Biotechnology,2015,99(8):3707-3713.
• [26] Niu T H,Zhou Z,Shen X L,et al.Effects of dissolved oxygen on performance and microbial community structure in a micro-aerobic hydrolysis sludge in situ reduction process[J].Water Research,2016,90:369-377.
• [27] Zhao L M,Zhang C C,Bao M T,et al.Advanced treatment for actual hydrolyzed polyacrylamide-containing wastewater in a biofilm/activated sludge membrane bioreactor system:Biodegradation and interception[J].Biochemical Engineering Journal,2019,141:120-130.
• [28] Wang Q,Ding J,Xie H,et al.Phosphorus removal performance of microbial-enhanced constructed wetlands that treat saline wastewater[J].Journal of Cleaner Production,2021,288:125119.
• [29] Hossain M I,Cheng L,Cord-Ruwisch R.Energy efficient COD and N-removal from high-strength wastewater by a passively aerated GAO dominated biofilm[J].Bioresource Technology,2019,283:148-158.
• [30] 韩亚琳，王福浩，王群，等.HSBBR运行模式对同步短程硝化反硝化脱氮及微生物群落特征的影响[J].环境工程，2021,39(1):51-57.Han Y L,Wang F H,Wang Q,et al.Effect of operational mode on nitrogen removal and microbial community in the process of simultaneous partial nitrification and denitrification[J].Environmental Engineering,2021,39(1):51-57.
• [31] 王旭，王福浩，吴澜，等.超高盐度对耐盐活性污泥脱氮、微生物活性和群落结构的影响[J].中国海洋大学学报(自然科学版),2021,51(S1):50-59.Wang X,Wang F H,Wu L,et al.Effects of ultra-high salinity on denitrification,microbial activity and community structure of salt-tolerant activated sludge[J].Journal of Ocean University of China(Natural Science),2021,51(S1):50-59.
• [32] Guo J B,Cong Q W,Zhang J,et al.Nitrous oxide emission in a laboratory anoxic-oxic process at different influent pHs:Generation pathways and the composition and function of bacterial community[J].Bioresource Technology,2021,328:124844.
• [33] Pronk M,Neu T R,van Loosdrecht M C M,et al.The acid soluble extracellular polymeric substance of aerobic granular sludge dominated by Defluviicoccus sp.[J].Water Research,2017,122:148-158.
• [34] Seviour T W,Lambert L K,Pijuan M,et al.Selectively inducing the synthesis of a key structural exopolysaccharide in aerobic granules by enriching for Candidatus “Competibacter phosphatis”[J].Applied Microbiology and Biotechnology,2011,92(6):1297-1305.

文章评论(Comment)：